Abstract
We earlier isolated a cDNA clone (NGR1) encoding a wound-inducible ribonuclease (RNase NW) from leaves of Nicotiana glutinosa [Kariu et al. Biosci. Biotechnol. Biochem., 62, 1144-1151 (1998)]. In this study, two distinct cDNA clones, NGR2 and NGR3, encoding proteins with a ribonuclease-related sequence in the N. glutinosa leaves, were amplified and sequenced. The nucleotide sequences of NGR2 and NGR3 consist of 1244 bp and 1069 bp, and have open reading frames encoding 277 (RNase NGR2) and 236 (RNase NGR3) amino acid residues, respectively. The deduced amino acid sequences of the putative RNases NGR2 and NGR3 showed 33% and 58% amino acid sequence identity, respectively, with that of RNase NW and 32% identity with each other. Sequence comparison showed that NGR2 is similar to RNase RNS2 (61%) from Arabidopsis thaliana, while NGR3 is related to RNase LX (84%) from tomato (Lycopersicon esculentum). RNA gel blot analysis showed that the RNase NGR2 gene is constitutively expressed to measurable levels; it is not increased by either wounding or TMV infection. In contrast, the expression of the NGR3 gene is induced after 48 h upon TMV infection.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Two distinct ribonuclease-related cDNA clones, NGR2 and NGR3, were cloned from Nicotiana glutinosa leaves.
- Taxon
- Nicotiana glutinosa
Nicotiana glutinosat0 - Organ / stage
- leavese1
- Gene
- NGR2 and NGR3e1
- Outcome
- two distinct cDNA clonese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e1“two distinct cDNA clones, NGR2 and NGR3, encoding proteins with a ribonuclease-related sequence in the N. glutinosa leaves”
Claim · model-written summary
The NGR2 nucleotide sequence is 1244 bp.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR2e2
- Outcome
- nucleotide sequencese2
- Value
- 1244 bpe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e2“The nucleotide sequences of NGR2 and NGR3 consist of 1244 bp and 1069 bp”
Claim · model-written summary
The NGR3 nucleotide sequence is 1069 bp.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR3e2
- Outcome
- nucleotide sequencese2
- Value
- 1069 bpe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e2“The nucleotide sequences of NGR2 and NGR3 consist of 1244 bp and 1069 bp”
Claim · model-written summary
Deduced NGR2 protein shares 33% amino acid identity with RNase NW.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR2e3
- Outcome
- amino acid sequence identitye3
- Value
- 33%e3
- Comparator
- RNase NWe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e3“The deduced amino acid sequences of the putative RNases NGR2 and NGR3 showed 33% and 58% amino acid sequence identity, respectively, with that of RNase NW”
Claim · model-written summary
Deduced NGR3 protein shares 58% amino acid identity with RNase NW.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR3e3
- Outcome
- amino acid sequence identitye3
- Value
- 58%e3
- Comparator
- RNase NWe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e3“The deduced amino acid sequences of the putative RNases NGR2 and NGR3 showed 33% and 58% amino acid sequence identity, respectively, with that of RNase NW”
Claim · model-written summary
NGR2 is similar to RNase RNS2 from Arabidopsis thaliana (61%).
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR2e4
- Outcome
- similare4
- Value
- 61%e4
- Comparator
- RNase RNS2e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e4“Sequence comparison showed that NGR2 is similar to RNase RNS2 (61%) from Arabidopsis thaliana”
Claim · model-written summary
NGR3 is related to RNase LX from tomato (84%).
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Gene
- NGR3e5
- Outcome
- relatede5
- Value
- 84%e5
- Comparator
- RNase LXe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e5“NGR3 is related to RNase LX (84%) from tomato (Lycopersicon esculentum)”
Claim · model-written summary
RNase NGR2 gene is constitutively expressed and not increased by wounding or TMV infection.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Treatment
- wounding or TMV infectione6
- Interactor
- TMV
Tobacco mosaic viruse6pathogen - Gene
- RNase NGR2 genee6
- Outcome
- constitutively expressed to measurable levelse6
- Direction
- not increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e6“RNA gel blot analysis showed that the RNase NGR2 gene is constitutively expressed to measurable levels; it is not increased by either wounding or TMV infection”
Claim · model-written summary
NGR3 gene expression is induced after 48 h of TMV infection.
- Taxon
- Nicotiana glutinosaNicotiana glutinosat0
- Treatment
- TMV infectione7
- Interactor
- TMVTobacco mosaic viruse7pathogen
- Gene
- NGR3e7
- Outcome
- expression of the NGR3 genee7
- Direction
- inducede7
- Qualifier
- after 48 he7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana glutinosa”
e7“the expression of the NGR3 gene is induced after 48 h upon TMV infection”
The same questions in other papers
infection · Tobacco mosaic virus · Nicotiana glutinosaPositive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.