Abstract
Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV). Crude extracts prepared from the resistant leaves were more inhibitory to TNV infection on Phaseolus than were extracts from non-resistant (control) leaves. Subsequently, crude extracts were fractionated into protein and ribonucleic acid (RNA) and inhibitory activity from resistant areas was retained in the RNA preparation. The RNA from resistant and non-resistant leaves was purified and separated into three fractions (about 27 S, 17 S, and 3 S), by gel filtration on Sephadex G-200. The 27-S and 17-S fractions from both treatments inhibited TNV infection by similar amounts. However, the 3-S RNA from resistant leaves reduced lesion number to a greater extent when compared to both the 3-S RNA from control leaves and the 27-S and 17-S RNA from either treatment. The lowest molecular weight RNA isolated from systemic-induced resistant areas gave positive tests with orcinol reagent and had an absorption spectrum characteristic of a nucleic acid. When treated with pancreatic ribonuclease and mixed with TNV, inhibition no longer occurred.
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Claims 7
Claim · model-written summary
Extracts from resistant leaves were more inhibitory to TNV infection on Phaseolus than extracts from non-resistant leaves.
- Taxon
- Phaseolus vulgaris var. Prince
Phaseolus vulgarise1 - Treatment
- localized infection with tobacco mosaic or necrosis virusese1
- Interactor
- TNVunidentified tobacco necrosis viruse2pathogen
- Outcome
- inhibitory to TNV infectione2
- Direction
- more inhibitorye2
- Comparator
- non-resistant (control) leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e2“Crude extracts prepared from the resistant leaves were more inhibitory to TNV infection on Phaseolus than were extracts from non-resistant (control) leaves”
Claim · model-written summary
The 3-S RNA from resistant leaves reduced lesion number more than 3-S RNA from control leaves.
- Taxon
- Phaseolus vulgaris var. PrincePhaseolus vulgarise1
- Treatment
- 3-S RNA from resistant leavese3
- Outcome
- lesion numbere3
- Direction
- reducede3
- Comparator
- 3-S RNA from control leavese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e3“the 3-S RNA from resistant leaves reduced lesion number to a greater extent when compared to both the 3-S RNA from control leaves and the 27-S and 17-S RNA from either treatment”
Claim · model-written summary
The 27-S and 17-S RNA fractions from both resistant and control leaves inhibited TNV infection by similar amounts.
- Taxon
- Phaseolus vulgaris var. PrincePhaseolus vulgarise1
- Treatment
- 27-S and 17-S fractionse4
- Interactor
- TNVunidentified tobacco necrosis viruse4pathogen
- Outcome
- inhibited TNV infectione4
- Direction
- similar amountse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e4“The 27-S and 17-S fractions from both treatments inhibited TNV infection by similar amounts”
Claim · model-written summary
Inhibitory activity from resistant leaves was retained in the RNA preparation after fractionation.
- Taxon
- Phaseolus vulgaris var. PrincePhaseolus vulgarise1
- Compound
- RNAe5
- Outcome
- inhibitory activitye5
- Direction
- retainede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e5“inhibitory activity from resistant areas was retained in the RNA preparation”
Claim · model-written summary
The lowest molecular weight RNA from systemic-induced resistant leaves gave positive orcinol tests and a nucleic-acid absorption spectrum.
- Taxon
- Phaseolus vulgaris var. PrincePhaseolus vulgarise1
- Compound
- RNAe6
- Outcome
- positive tests with orcinol reagente6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e6“The lowest molecular weight RNA isolated from systemic-induced resistant areas gave positive tests with orcinol reagent”
Claim · model-written summary
After pancreatic ribonuclease treatment, the RNA-mediated inhibition of TNV no longer occurred.
- Taxon
- Phaseolus vulgaris var. PrincePhaseolus vulgarise1
- Treatment
- pancreatic ribonucleasee7
- Interactor
- TNVunidentified tobacco necrosis viruse7pathogen
- Outcome
- inhibitione7
- Direction
- no longer occurrede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
e7“When treated with pancreatic ribonuclease and mixed with TNV, inhibition no longer occurred”
Claim · model-written summary
Localized infection with TMV or TNV induced systemic resistance in the leaves of four test plants.
- Taxon
- Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolorPhaseolus vulgaris
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Datura stramonium
Nicotiana glutinosa
Chenopodium amaranticolore1 - Treatment
- localized infection with tobacco mosaic or necrosis virusese1
- Interactor
- TMV
Tobacco mosaic viruse1pathogen - Second interactor
- TNVunidentified tobacco necrosis viruse1pathogen
- Outcome
- Systemic resistancee1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Systemic resistance was induced in the leaves of Datura stramonium L., Nicotiana glutinosa, Phaseolus vulgaris var. Prince, and Chenopodium amaranticolor by localized infection with tobacco mosaic or necrosis viruses (TMV, TNV)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.