Abstract
The cloning and characterization of genes expressed in plant disease resistance could be an initial step toward understanding the molecular mechanisms of disease resistance. A metallothionein-like gene that is inducible by tobacco mosaic virus and by wounding was cloned in the process of subtractive cloning of disease resistance-response genes in Nicotiana glutinosa. One 530-bp cDNA clone (KC9-10) containing an open reading frame of 81 amino acids was characterized. Genomic Southern blot hybridization with the cDNA probe revealed that tobacco metallothionein-like genes are present in few or in one copy per diploid genome. Northern blot hybridization detected strong induction of a 0.5-kb mRNA by wounding and tobacco mosaic virus infection, but only mild induction was detected when copper was tested as an inducer. Methyl jasmonate, salicylic acid, and ethylene were also tested as possible inducers of this gene, but they had no effect on its expression. The possible role of this gene in wounded and pathogen-stressed plants is discussed.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
A metallothionein-like gene inducible by tobacco mosaic virus and wounding was cloned.
- Taxon
- Nicotiana glutinosa
Nicotiana glutinosae0 - Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Gene
- metallothionein-like genee0
- Outcome
- was clonede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A metallothionein-like gene that is inducible by tobacco mosaic virus and by wounding was cloned in the process of subtractive cloning of disease resistance-response genes in Nicotiana glutinosa.”
Claim · model-written summary
The characterized KC9-10 clone was 530 bp with an 81-amino-acid open reading frame.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Gene
- KC9-10e0
- Outcome
- open reading framee0
- Value
- 81 amino acidse0
- Qualifier
- 530-bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One 530-bp cDNA clone (KC9-10) containing an open reading frame of 81 amino acids was characterized.”
t0“Nicotiana glutinosa L.”
Claim · model-written summary
Tobacco metallothionein-like genes occur in few or one copy per diploid genome.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Gene
- tobacco metallothionein-like genese0
- Outcome
- few or in one copy per diploid genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco metallothionein-like genes are present in few or in one copy per diploid genome”
t0“Nicotiana glutinosa L.”
Claim · model-written summary
Wounding strongly induced a 0.5-kb mRNA.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Treatment
- woundinge0
- Gene
- 0.5-kb mRNAe0
- Outcome
- 0.5-kb mRNAe0
- Direction
- strong inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong induction of a 0.5-kb mRNA by wounding”
t0“Nicotiana glutinosa L.”
Claim · model-written summary
Tobacco mosaic virus infection strongly induced a 0.5-kb mRNA.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Treatment
- tobacco mosaic virus infectione0
- Interactor
- tobacco mosaic virusTobacco mosaic viruse0pathogen
- Outcome
- 0.5-kb mRNAe0
- Direction
- strong inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong induction of a 0.5-kb mRNA by wounding and tobacco mosaic virus infection”
t0“Nicotiana glutinosa L.”
Claim · model-written summary
Copper caused only mild induction of the mRNA.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Treatment
- coppere0
- Outcome
- 0.5-kb mRNAe1
- Direction
- mild inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only mild induction was detected when copper was tested as an inducer”
e1“0.5-kb mRNA by wounding and tobacco mosaic virus infection”
t0“Nicotiana glutinosa L.”
Claim · model-written summary
Methyl jasmonate, salicylic acid and ethylene had no effect on gene expression.
- Taxon
- Nicotiana glutinosa L.Nicotiana glutinosat0
- Treatment
- Methyl jasmonate, salicylic acid, and ethylenee0
- Outcome
- expressione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methyl jasmonate, salicylic acid, and ethylene were also tested as possible inducers of this gene, but they had no effect on its expression.”
t0“Nicotiana glutinosa L.”
The same questions in other papers
ethene · molecular: expression · Nicotiana glutinosaPositive and negative results
1 in the opposite direction
- positive
(-)-methyl jasmonate · molecular: expression · Nicotiana glutinosaPositive and negative results
2 in the opposite direction
- positive
- positive
salicylic acid · molecular: expression · Nicotiana glutinosaPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Nicotiana tabacum · Tobacco mosaic virus · Nicotiana glutinosaPositive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →infection · Tobacco mosaic virus · Nicotiana glutinosaPositive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.