Abstract
For a better understanding of shoot branching in rice (Oryza sativa), a rice activation-tagging library was screened for mutations in tiller development. Here, an activation-tagging mutant Ostil1 (Oryza sativa tillering1) was characterized, which showed increased tillers, enlarged tiller angle and semidwarf phenotype. Flanking sequence was obtained by plasmid rescue. RNA-interfering and overexpression transgenic rice plants were produced using Agrobacterium-mediated transformation. The mutant phenotype was cosegregated with the reallocation of Ds element, and the flanking region of the reallocated Ds element was identified as part of the OsNAC2 gene. Northern analysis showed that expression of OsNAC2 was greatly induced in the mutant plants. Transgenic rice overexpressing the OsNAC2 resulted in recapture of the mutant phenotype, while downregulation of OsNAC2 in the Ostil1 mutant through RNA interfering (RNAi) complemented the mutant phenotype, confirming that the Ostil1 was caused by overexpression of OsNAC2. Overexpression of OsNAC2 regulates shoot branching in rice. Overexpression of OsNAC2 contributes tiller bud outgrowth, but does not affect tiller bud initiation. This suggests that OsNAC2 has potential utility for improving plant structure for higher light-use efficiency and higher yield potential in rice.
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Claims 11
Claim · model-written summary
The Ostil1 rice mutant has increased tillers and an enlarged tiller angle.
- Taxon
- rice
Oryza sativat0 - Material
- Ostil1e0
- Outcome
- increased tillers, enlarged tiller anglee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Here, an activation-tagging mutant Ostil1 (Oryza sativa tillering1) was characterized, which showed increased tillers, enlarged tiller angle and semidwarf phenotype.”
Claim · model-written summary
The Ostil1 rice mutant has a semidwarf phenotype.
- Taxon
- riceOryza sativat0
- Material
- Ostil1e0
- Outcome
- semidwarf phenotypee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Here, an activation-tagging mutant Ostil1 (Oryza sativa tillering1) was characterized, which showed increased tillers, enlarged tiller angle and semidwarf phenotype.”
Claim · model-written summary
The rice mutant phenotype cosegregates with Ds element reallocation.
- Taxon
- riceOryza sativat0
- Gene
- Ds elemente0
- Outcome
- mutant phenotypee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“The mutant phenotype was cosegregated with the reallocation of Ds element”
Claim · model-written summary
The region flanking the reallocated Ds element is part of rice OsNAC2.
- Taxon
- riceOryza sativat0
- Gene
- OsNAC2e0
- Outcome
- identified as part of the OsNAC2 genee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“the flanking region of the reallocated Ds element was identified as part of the OsNAC2 gene.”
Claim · model-written summary
OsNAC2 expression is greatly induced in mutant rice plants.
- Taxon
- riceOryza sativat0
- Material
- mutant plantse0
- Gene
- OsNAC2e0
- Outcome
- expression of OsNAC2e0
- Direction
- greatly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Northern analysis showed that expression of OsNAC2 was greatly induced in the mutant plants.”
Claim · model-written summary
Rice OsNAC2 overexpression recaptures the Ostil1 mutant phenotype.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing the OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- recapture of the mutant phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice overexpressing the OsNAC2 resulted in recapture of the mutant phenotype”
Claim · model-written summary
RNA interference downregulation of OsNAC2 in Ostil1 complements the mutant phenotype.
- Taxon
- riceOryza sativat0
- Material
- Ostil1e0
- Treatment
- downregulation of OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- complemented the mutant phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“downregulation of OsNAC2 in the Ostil1 mutant through RNA interfering (RNAi) complemented the mutant phenotype”
Claim · model-written summary
OsNAC2 overexpression causes the Ostil1 rice phenotype.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- Ostil1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“confirming that the Ostil1 was caused by overexpression of OsNAC2.”
Claim · model-written summary
OsNAC2 overexpression promotes rice tiller bud outgrowth.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- tiller bud outgrowthe0
- Direction
- contributese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Overexpression of OsNAC2 contributes tiller bud outgrowth, but does not affect tiller bud initiation.”
Claim · model-written summary
OsNAC2 overexpression does not affect rice tiller bud initiation.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- tiller bud initiatione0
- Direction
- does not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Overexpression of OsNAC2 contributes tiller bud outgrowth, but does not affect tiller bud initiation.”
Claim · model-written summary
OsNAC2 has potential use in improving rice plant structure for greater light-use efficiency and yield potential.
- Taxon
- riceOryza sativae0
- Gene
- OsNAC2e0
- Outcome
- improving plant structuree0
- Qualifier
- potential utilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests that OsNAC2 has potential utility for improving plant structure for higher light-use efficiency and higher yield potential in rice.”