Abstract
In Sinorhizobium meliloti the NolR repressor displays differential negative regulation of nodulation genes and is required for optimal nodulation. Here, we demonstrate that the NolR function is not unique to S. meliloti but is also present in other species of the Rhizobiaceae family. DNA hybridization indicates the presence of nolR homologous sequences in species belonging to the Rhizobium and Sinorhizobium genera while no hybridization signal was detected in species from the Mesorhizobium, Bradyrhizo-bium, Azorhizobium, and Agrobacterium genera. We isolated the nolR gene from the Rhizobium leguminosarum bv. viciae strain TOM and showed that the TOM nolR gene acts similarly to S. meliloti nolR by repressing the expression of both the nodABCIJ and the nodD genes, resulting in decreased Nod factor production. The presence of a functional nolR gene in R. leguminosarum is correlated with an increased rate and extent of nodulation of pea. The conserved primary structure, the location of the DNA-binding domain, and the similar size of NolR proteins, compared with a family of small bacterial regulatory proteins including HlyU, SmtB, and the ArsR-type regulators, revealed that NolR belongs to this family.
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Claims 7
Claim · model-written summary
NolR function occurs in other Rhizobiaceae species.
- Taxon
- RhizobiaceaeRhizobiaceaee0
- Gene
- NolRe0
- Outcome
- NolR functione0
Verbatim
e0“the NolR function is not unique to S. meliloti but is also present in other species of the Rhizobiaceae family”
Claim · model-written summary
nolR homologous sequences were detected in Rhizobium and Sinorhizobium.
- Taxon
- Rhizobium and Sinorhizobium
Rhizobium.JPG?width=60)
Sinorhizobiume0 - Gene
- nolRe0
- Outcome
- presence of nolR homologous sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“presence of nolR homologous sequences in species belonging to the Rhizobium and Sinorhizobium genera”
Claim · model-written summary
No hybridization signal was found in the other listed genera.
- Taxon
- Mesorhizobium, Bradyrhizo-bium, Azorhizobium, and Agrobacterium
Azorhizobium.jpg?width=60)
Agrobacterium
Bradyrhizobium.jpg?width=60)
Mesorhizobiume0 - Outcome
- no hybridization signale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
.jpg?width=500)
Verbatim
e0“no hybridization signal was detected in species from the Mesorhizobium, Bradyrhizo-bium, Azorhizobium, and Agrobacterium genera”
Claim · model-written summary
The TOM nolR gene represses nodABCIJ and nodD expression.
- Taxon
- Rhizobium leguminosarum bv. viciae strain TOMRhizobium leguminosarum bv. viciae TOMe1
- Gene
- TOM nolRe0
- Outcome
- expression of both the nodABCIJ and the nodD genese0
Verbatim
e0“the TOM nolR gene acts similarly to S. meliloti nolR by repressing the expression of both the nodABCIJ and the nodD genes”
e1“Rhizobium leguminosarum bv. viciae strain TOM”
Claim · model-written summary
nolR repression decreased Nod factor production.
- Taxon
- Rhizobium leguminosarum bv. viciae strain TOMRhizobium leguminosarum bv. viciae TOMe1
- Gene
- nolRe1
- Outcome
- Nod factor productione0
- Direction
- decreasede0
Verbatim
e0“resulting in decreased Nod factor production”
e1“the Rhizobium leguminosarum bv. viciae strain TOM and showed that the TOM nolR gene acts similarly to S. meliloti nolR by repressing the expression of both the nodABCIJ and the nodD genes”
Claim · model-written summary
Functional nolR correlates with increased rate and extent of pea nodulation.
- Taxon
- pea
Lathyrus oleraceuse0 - Interactor
- R. leguminosarum
Rhizobium leguminosarume0symbionte1 - Gene
- nolRe0
- Outcome
- rate and extent of nodulatione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of a functional nolR gene in R. leguminosarum is correlated with an increased rate and extent of nodulation of pea”
e1“Rhizobium leguminosarum bv. viciae strain TOM”
Claim · model-written summary
NolR was placed in a family of small bacterial regulatory proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NolR belongs to this family”
t0“Rhizobium”