Abstract
Cotton fibres are unusually long, single-celled epidermal seed trichomes and a model for plant cell growth, but little is known about the regulation of fibre cell elongation. Here we report that a homeodomain-leucine zipper (HD-ZIP) transcription factor, GhHOX3, controls cotton fibre elongation. GhHOX3 genes are localized to the 12th homoeologous chromosome set of allotetraploid cotton cultivars, associated with quantitative trait loci (QTLs) for fibre length. Silencing of GhHOX3 greatly reduces (>80%) fibre length, whereas its overexpression leads to longer fibre. Combined transcriptomic and biochemical analyses identify target genes of GhHOX3 that also contain the L1-box cis-element, including two cell wall loosening protein genes GhRDL1 and GhEXPA1. GhHOX3 interacts with GhHD1, another homeodomain protein, resulting in enhanced transcriptional activity, and with cotton DELLA, GhSLR1, repressor of the growth hormone gibberellin (GA). GhSLR1 interferes with the GhHOX3-GhHD1 interaction and represses target gene transcription. Our results uncover a novel mechanism whereby a homeodomain protein transduces GA signal to promote fibre cell elongation.
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Claims 8
Claim · model-written summary
GhHOX3 genes occur on the 12th homoeologous chromosome set and associate with fibre length QTLs.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- GhHOX3e0
- Outcome
- quantitative trait loci (QTLs) for fibre lengthe0
- Qualifier
- 12th homoeologous chromosome sete0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhHOX3 genes are localized to the 12th homoeologous chromosome set of allotetraploid cotton cultivars, associated with quantitative trait loci (QTLs) for fibre length”
Claim · model-written summary
Silencing GhHOX3 reduced fibre length by over 80%.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Silencing of GhHOX3e0
- Gene
- GhHOX3e0
- Outcome
- fibre lengthe0
- Direction
- reducese0
- Value
- >80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GhHOX3 greatly reduces (>80%) fibre length”
t0“cotton”
Claim · model-written summary
GhHOX3 overexpression lengthened fibre.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- overexpressione0
- Outcome
- fibree0
- Direction
- longere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its overexpression leads to longer fibre”
t0“cotton”
Claim · model-written summary
GhRDL1 and GhEXPA1 were identified as GhHOX3 targets containing L1-box.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhRDL1 and GhEXPA1e0
- Outcome
- target genes of GhHOX3 that also contain the L1-box cis-elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“target genes of GhHOX3 that also contain the L1-box cis-element, including two cell wall loosening protein genes GhRDL1 and GhEXPA1”
t0“cotton”
Claim · model-written summary
GhHOX3 interacted with GhHD1 and enhanced transcriptional activity.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhHOX3e0
- Outcome
- enhanced transcriptional activitye0
- Qualifier
- interacts with GhHD1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhHOX3 interacts with GhHD1, another homeodomain protein, resulting in enhanced transcriptional activity”
t0“cotton”
Claim · model-written summary
GhHOX3 interacted with GhSLR1, a GA repressor.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhSLR1e0
- Outcome
- repressor of the growth hormone gibberellin (GA)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with cotton DELLA, GhSLR1, repressor of the growth hormone gibberellin (GA)”
Claim · model-written summary
GhSLR1 interfered with the GhHOX3-GhHD1 interaction.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhSLR1e0
- Outcome
- GhHOX3-GhHD1 interactione0
- Qualifier
- interferese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhSLR1 interferes with the GhHOX3-GhHD1 interaction and represses target gene transcription”
t0“cotton”
Claim · model-written summary
GhSLR1 repressed target gene transcription.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhSLR1e0
- Outcome
- target gene transcriptione0
- Qualifier
- repressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhSLR1 interferes with the GhHOX3-GhHD1 interaction and represses target gene transcription”
t0“cotton”
The same questions in other papers
loss of function · quality: grain · Gossypium hirsutum (cotton)Positive and negative results
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- negative
- negative
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See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.