Abstract
Cotton is the most important textile crop as a result of its long cellulose-enriched mature fibers. These single-celled hairs initiate at anthesis from the ovule epidermis. To date, genes proven to be critical for fiber development have not been identified. Here, we examined the role of the sucrose synthase gene (Sus) in cotton fiber and seed by transforming cotton with Sus suppression constructs. We focused our analysis on 0 to 3 days after anthesis (DAA) for early fiber development and 25 DAA, when the fiber and seed are maximal in size. Suppression of Sus activity by 70% or more in the ovule epidermis led to a fiberless phenotype. The fiber initials in those ovules were fewer and shrunken or collapsed. The level of Sus suppression correlated strongly with the degree of inhibition of fiber initiation and elongation, probably as a result of the reduction of hexoses. By 25 DAA, a portion of the seeds in the fruit showed Sus suppression only in the seed coat fibers and transfer cells but not in the endosperm and embryo. These transgenic seeds were identical to wild-type seeds except for much reduced fiber growth. However, the remaining seeds in the fruit showed Sus suppression both in the seed coat and in the endosperm and embryo. These seeds were shrunken with loss of the transfer cells and were <5% of wild-type seed weight. These results demonstrate that Sus plays a rate-limiting role in the initiation and elongation of the single-celled fibers. These analyses also show that suppression of Sus only in the maternal seed tissue represses fiber development without affecting embryo development and seed size. Additional suppression in the endosperm and embryo inhibits their own development, which blocks the formation of adjacent seed coat transfer cells and arrests seed development entirely.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
At least 70% Sus activity suppression in ovule epidermis produced fiberless ovules.
- Taxon
- Cotton
Gossypium hirsutumt0 - Organ / stage
- ovule epidermise0
- Treatment
- Suppression of Sus activity by 70% or moree0
- Gene
- Suse0
- Outcome
- fiberless phenotypee0
- Value
- 70% or moree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of Sus activity by 70% or more in the ovule epidermis led to a fiberless phenotype”
t0“Cotton Fiber”
Claim · model-written summary
Fiber initials in suppressed ovules were fewer and shrunken or collapsed.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Suppression of Sus activity by 70% or moree1
- Gene
- Suse1
- Outcome
- fiber initialse0
- Direction
- fewere0
- Qualifier
- shrunken or collapsede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fiber initials in those ovules were fewer and shrunken or collapsed”
e1“Suppression of Sus activity by 70% or more in the ovule epidermis led to a fiberless phenotype”
t0“Cotton Fiber”
Claim · model-written summary
Greater Sus suppression correlated with stronger inhibition of fiber initiation and elongation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- level of Sus suppressione0
- Gene
- Suse0
- Outcome
- inhibition of fiber initiation and elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of Sus suppression correlated strongly with the degree of inhibition of fiber initiation and elongation”
t0“Cotton Fiber”
Claim · model-written summary
Reduced hexoses probably explain inhibition of fiber initiation and elongation.
- Taxon
- CottonGossypium hirsutumt0
- Compound
- hexosese0
- Outcome
- inhibition of fiber initiation and elongatione0
- Qualifier
- probablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of fiber initiation and elongation, probably as a result of the reduction of hexoses”
t0“Cotton Fiber”
Claim · model-written summary
Seeds with Sus suppression confined to seed coat fibers and transfer cells had reduced fiber growth.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Sus suppression only in the seed coat fibers and transfer cellse1
- Gene
- Suse1
- Outcome
- fiber growthe0
- Direction
- reducede0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These transgenic seeds were identical to wild-type seeds except for much reduced fiber growth”
e1“Sus suppression only in the seed coat fibers and transfer cells but not in the endosperm and embryo”
t0“Cotton Fiber”
Claim · model-written summary
Seeds with suppression confined to maternal tissue otherwise matched wild-type seeds.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Sus suppression only in the seed coat fibers and transfer cellse1
- Gene
- Suse1
- Outcome
- transgenic seedse0
- Direction
- identicale0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These transgenic seeds were identical to wild-type seeds except for much reduced fiber growth”
e1“Sus suppression only in the seed coat fibers and transfer cells but not in the endosperm and embryo”
t0“Cotton Fiber”
Claim · model-written summary
Seeds with Sus suppression in both maternal and embryo tissues were shrunken and lost transfer cells.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Sus suppression both in the seed coat and in the endosperm and embryoe1
- Gene
- Suse1
- Outcome
- shrunken with loss of the transfer cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These seeds were shrunken with loss of the transfer cells”
e1“Sus suppression both in the seed coat and in the endosperm and embryo”
t0“Cotton Fiber”
Claim · model-written summary
Seeds with wider Sus suppression weighed under 5% of wild type.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Sus suppression both in the seed coat and in the endosperm and embryoe1
- Gene
- Suse1
- Outcome
- seed weighte0
- Direction
- <e0
- Value
- <5%e0
- Comparator
- wild-type seede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These seeds were shrunken with loss of the transfer cells and were <5% of wild-type seed weight”
e1“Sus suppression both in the seed coat and in the endosperm and embryo”
t0“Cotton Fiber”
Claim · model-written summary
Sus has a limiting role in fiber initiation and elongation.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- Suse0
- Outcome
- initiation and elongation of the single-celled fiberse0
- Qualifier
- rate-limiting rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sus plays a rate-limiting role in the initiation and elongation of the single-celled fibers”
t0“Cotton Fiber”
The same questions in other papers
loss of function · quality: grain · Gossypium hirsutum (cotton)Positive and negative results
29 papers in the same direction as this paper · 11 in the opposite direction
- negative
- negative
- negative
37 papers more in this group
See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.