Paper · 2002 · Journal of the Science of Food and Agriculture

Effects of malting on β‐glucanase and phytase activity in barley grain

Lena Rimsten; Ann‐Katrin Haraldsson; Roger Andersson; et al.

10
claims
22
verbatim quotes
62
citations
1
research areas
—
Not open access

Abstract

Abstract The effects of malting on β‐glucan and phytate were investigated in one naked and one covered barley by a full factorial experiment with three factors (steeping temperature, moisture content and germination temperature) each with two levels. Analysis of total content of β‐glucan in the malted samples showed small changes after steeping at the high temperature (48 °C), while steeping at the lower temperature (15 °C) gave a significantly lower content. This trend was even stronger for β‐glucan unextractable at 38 °C. Analysis of the activity of β‐glucanase for the samples steeped at 15 °C showed a strong increase over the time of germination, while those steeped at 48 °C had a much slower development. The other two factors influenced the outcome to a small extent, mainly because the steeping temperature was the most important factor overall where any changes in β‐glucan and β‐glucanase were observed. When β‐glucan was extracted at 100 °C, a larger yield was obtained, and this was influenced by the steeping temperature in a much stronger way than for β‐glucan extracted at 38 °C. Determination of average molecular weight for β‐glucan extracted at 100 °C gave a lower value for samples steeped at 15 than at 48 °C. The design did not have any large effects on phytate degradation and phytase activity. However, it indicated that selective control of the enzymes might be possible, since phytase activity was barely affected by the parameters studied, while β‐glucanase was heavily affected. © 2002 Society of Chemical Industry

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Claims 10

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Steeping at the lower temperature (15 °C) gave a significantly lower total beta-glucan content than steeping at the high temperature (48 °C).

Treatment
steeping at the lower temperature (15 °C)e0
Outcome
total content of β‐glucane1
Direction
significantly lowere0
Comparator
steeping at the high temperature (48 °C)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e0

“steeping at the lower temperature (15 °C) gave a significantly lower content”

✓ located verbatim in the source · abstract, characters 386–462 · show in text ↑
e1

“Analysis of total content of β‐glucan in the malted samples showed small changes after steeping at the high temperature (48 °C)”

✓ located verbatim in the source · abstract, characters 251–378 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Steeping at 48 °C caused only small changes in total beta-glucan content.

Treatment
steeping at the high temperature (48 °C)e1
Outcome
total content of β‐glucane1
Qualifier
small changese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e1

“Analysis of total content of β‐glucan in the malted samples showed small changes after steeping at the high temperature (48 °C)”

✓ located verbatim in the source · abstract, characters 251–378 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

For samples steeped at 15 °C, beta-glucanase activity showed a strong increase over germination time.

Treatment
samples steeped at 15 °Ce2
Outcome
activity of β‐glucanasee2
Direction
strong increasee2
Qualifier
over the time of germinatione2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e2

“Analysis of the activity of β‐glucanase for the samples steeped at 15 °C showed a strong increase over the time of germination, while those steeped at 48 °C had a much slower development”

✓ located verbatim in the source · abstract, characters 530–716 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

For samples steeped at 48 °C, beta-glucanase activity developed much more slowly during germination.

Treatment
steeped at 48 °Ce2
Outcome
activity of β‐glucanasee2
Direction
much slower developmente2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e2

“Analysis of the activity of β‐glucanase for the samples steeped at 15 °C showed a strong increase over the time of germination, while those steeped at 48 °C had a much slower development”

✓ located verbatim in the source · abstract, characters 530–716 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Beta-glucan extracted at 100 °C gave a larger yield, and this was influenced by steeping temperature much more strongly than beta-glucan extracted at 38 °C.

Treatment
steeping temperaturee4
Outcome
yielde4
Direction
largere4
Qualifier
extracted at 100 °Ce4
Comparator
extracted at 38 °Ce4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e4

“When β‐glucan was extracted at 100 °C, a larger yield was obtained, and this was influenced by the steeping temperature in a much stronger way than for β‐glucan extracted at 38 °C”

✓ located verbatim in the source · abstract, characters 921–1100 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Beta-glucan extracted at 100 °C had a lower average molecular weight in samples steeped at 15 °C than at 48 °C.

Treatment
samples steeped at 15e5
Outcome
average molecular weighte5
Direction
lowere5
Comparator
at 48 °Ce5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e5

“Determination of average molecular weight for β‐glucan extracted at 100 °C gave a lower value for samples steeped at 15 than at 48 °C”

✓ located verbatim in the source · abstract, characters 1102–1235 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Steeping temperature, moisture content and germination temperature did not have any large effects on phytate degradation or phytase activity.

Treatment
steeping temperature, moisture content and germination temperaturee7
Outcome
phytate degradation and phytase activitye6
Direction
did not have any large effectse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e6

“The design did not have any large effects on phytate degradation and phytase activity”

✓ located verbatim in the source · abstract, characters 1237–1322 · show in text ↑
e7

“a full factorial experiment with three factors (steeping temperature, moisture content and germination temperature) each with two levels”

✓ located verbatim in the source · abstract, characters 113–249 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Phytase activity was barely affected by the parameters studied.

Treatment
parameters studiede2
Outcome
phytase activitye2
Direction
barely affectede2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e2

“phytase activity was barely affected by the parameters studied, while β‐glucanase was heavily affected”

✓ located verbatim in the source · abstract, characters 1409–1511 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Beta-glucanase activity was heavily affected by the parameters studied.

Treatment
parameters studiede2
Outcome
β‐glucanasee2
Direction
heavily affectede2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e2

“phytase activity was barely affected by the parameters studied, while β‐glucanase was heavily affected”

✓ located verbatim in the source · abstract, characters 1409–1511 · show in text ↑
Recommendationhypothesisoriginal resultbasis n/a

Claim · model-written summary

Selective control of the enzymes might be possible in malting.

Outcome
selective control of the enzymese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Effects of malting on β‐glucanase and phytase activity in barley grain”

✓ located verbatim in the source · title, characters 0–70 · show in text ↑
e1

“it indicated that selective control of the enzymes might be possible”

✓ located verbatim in the source · abstract, characters 1333–1401 · show in text ↑