Paper · 2010 · Plant Biotechnology

Efficient induction of flower-color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

Yoshihiro Hase; Masachika Okamura; Daigaku Takeshita; et al.

5
claims
10
verbatim quotes
47
citations
3
research areas
—
Not open access

Abstract

We examined the effect of pretreatment on the frequency of flower-color mutants induced by ion beams. We found that petunia seedlings treated with 3% sucrose from 8 days after sowing accumulated significant amount of pigments within 4 days compared to non-treated control seedlings. The petunia seedlings treated with sucrose were exposed to 320-MeV carbon ions. The sucrose treatment did not affect the survival rate and seed fertility of the M1 plants. In the M2 lines obtained by self-pollination of individual M1 plants, chlorophyll mutants were obtained in both treated and non-treated groups with a similar frequency. Flower-color mutants that included magenta, purple, light pink and white were obtained from the original violet color. The frequency of flower-color mutants was significantly higher in the sucrose-treated group than in the non-treated group. These results suggest that sucrose pretreatment specifically increases the frequency of flower-color mutation following ion beam irradiation.

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Claims 5

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Petunia seedlings treated with 3% sucrose accumulated a significant amount of pigments within 4 days compared with non-treated control seedlings.

Taxon
petuniaPetuniae0
Treatment
3% sucrose from 8 days after sowinge0
Outcome
amount of pigmentse0
Direction
accumulatede0
Qualifier
within 4 dayse0
Comparator
non-treated control seedlingse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“petunia seedlings treated with 3% sucrose from 8 days after sowing accumulated significant amount of pigments within 4 days compared to non-treated control seedlings”

✓ located verbatim in the source · abstract, characters 116–281 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Sucrose-treated petunia seedlings had a significantly higher frequency of flower-color mutants than non-treated seedlings after ion beam irradiation.

Taxon
petuniaPetuniat0
Treatment
sucrose-treated groupe1
Outcome
frequency of flower-color mutantse1
Direction
highere1
Comparator
non-treated groupe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

t0

“petunia seedlings treated with high sucrose concentration”

✓ located verbatim in the source · title, characters 71–128 · show in text ↑
e1

“The frequency of flower-color mutants was significantly higher in the sucrose-treated group than in the non-treated group”

✓ located verbatim in the source · abstract, characters 743–864 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Sucrose treatment did not affect the survival rate or seed fertility of the M1 petunia plants.

Taxon
petuniaPetuniat0
Material
M1 plantse2
Treatment
sucrose treatmente2
Outcome
survival rate and seed fertilitye2
Direction
did not affecte2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

t0

“petunia seedlings treated with high sucrose concentration”

✓ located verbatim in the source · title, characters 71–128 · show in text ↑
e2

“The sucrose treatment did not affect the survival rate and seed fertility of the M1 plants”

✓ located verbatim in the source · abstract, characters 363–453 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Chlorophyll mutants arose in sucrose-treated and non-treated petunia groups at a similar frequency.

Taxon
petuniaPetuniat0
Treatment
3% sucrosee0
Outcome
chlorophyll mutantse3
Direction
similare3
Comparator
non-treated groupse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

t0

“petunia seedlings treated with high sucrose concentration”

✓ located verbatim in the source · title, characters 71–128 · show in text ↑
e0

“petunia seedlings treated with 3% sucrose from 8 days after sowing”

✓ located verbatim in the source · abstract, characters 116–182 · show in text ↑
e3

“chlorophyll mutants were obtained in both treated and non-treated groups with a similar frequency”

✓ located verbatim in the source · abstract, characters 525–622 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Flower-color mutants of petunia included magenta, purple, light pink and white, derived from the original violet color.

Taxon
petuniaPetuniat0
Outcome
flower-color mutantse4
Qualifier
magenta, purple, light pink and whitee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

t0

“petunia seedlings treated with high sucrose concentration”

✓ located verbatim in the source · title, characters 71–128 · show in text ↑
e4

“Flower-color mutants that included magenta, purple, light pink and white were obtained from the original violet color”

✓ located verbatim in the source · abstract, characters 624–741 · show in text ↑