Abstract
The genome of the Mastreviruses encodes a replication-associated protein (RepA) that interacts with members of the plant retinoblastoma-related protein family, which are putative cell cycle regulators. Expression of ZmRb1, a maize retinoblastoma-related gene, and RepA inhibited and stimulated, respectively, cell division in tobacco cell cultures. The effect of RepA was mitigated by over-expression of ZmRb1. RepA increased transformation frequency and callus growth rate of high type II maize germplasm. RepA-containing transgenic maize calli remained embryogenic, were readily regenerable, and produced fertile plants that transmitted transgene expression in a Mendelian fashion. In high type II, transformation frequency increased with the strength of the promoter driving RepA expression. When a construct in which RepA was expressed behind its native LIR promoter was used, primary transformation frequencies did not improve for two elite Pioneer maize inbreds. However, when LIR:RepA-containing transgenic embryos were used in subsequent rounds of transformation, frequencies were higher in the RepA+ embryos. These data demonstrate that RepA can stimulate cell division and callus growth in culture, and improve maize transformation.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Expression of ZmRb1 inhibited cell division in tobacco cell cultures.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- Expression of ZmRb1e1
- Gene
- ZmRb1e1
- Outcome
- cell divisione1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Expression of ZmRb1, a maize retinoblastoma-related gene, and RepA inhibited and stimulated, respectively, cell division in tobacco cell cultures”
Claim · model-written summary
RepA stimulated cell division in tobacco cell cultures.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Expression of ZmRb1, a maize retinoblastoma-related gene, and RepA inhibited and stimulated, respectively, cell division in tobacco cell cultures”
Claim · model-written summary
Over-expression of ZmRb1 mitigated the effect of RepA in maize.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e2“The effect of RepA was mitigated by over-expression of ZmRb1”
Claim · model-written summary
RepA increased transformation frequency and callus growth rate of high type II maize germplasm.
- Material
- high type IIe3
- Treatment
- RepAe3
- Gene
- RepAe3
- Outcome
- transformation frequency and callus growth ratee3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“RepA increased transformation frequency and callus growth rate of high type II maize germplasm”
Claim · model-written summary
RepA-containing transgenic maize calli remained embryogenic and were readily regenerable.
- Organ / stage
- callie4
- Treatment
- RepA-containinge4
- Outcome
- remained embryogenic, were readily regenerablee4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“RepA-containing transgenic maize calli remained embryogenic, were readily regenerable, and produced fertile plants that transmitted transgene expression in a Mendelian fashion”
Claim · model-written summary
RepA-containing transgenic maize plants were fertile and transmitted transgene expression in a Mendelian fashion.
- Treatment
- RepA-containinge4
- Outcome
- produced fertile plants that transmitted transgene expressione4
- Qualifier
- in a Mendelian fashione4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“RepA-containing transgenic maize calli remained embryogenic, were readily regenerable, and produced fertile plants that transmitted transgene expression in a Mendelian fashion”
Claim · model-written summary
In high type II maize, transformation frequency increased with the strength of the promoter driving RepA expression.
- Material
- high type IIe5
- Treatment
- strength of the promoter driving RepA expressione5
- Outcome
- transformation frequencye5
- Direction
- increasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e5“In high type II, transformation frequency increased with the strength of the promoter driving RepA expression”
Claim · model-written summary
RepA expressed behind its native LIR promoter did not improve primary transformation frequencies in two elite Pioneer maize inbreds.
- Material
- two elite Pioneer maize inbredse6
- Treatment
- RepA was expressed behind its native LIR promotere6
- Outcome
- primary transformation frequenciese6
- Direction
- did not improvee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“When a construct in which RepA was expressed behind its native LIR promoter was used, primary transformation frequencies did not improve for two elite Pioneer maize inbreds”
Claim · model-written summary
Transformation frequencies were higher in RepA+ embryos than in the LIR:RepA-containing transgenic embryos used in subsequent rounds of transformation.
- Material
- LIR:RepA-containing transgenic embryose7
- Treatment
- subsequent rounds of transformatione7
- Gene
- RepAe7
- Outcome
- frequenciese7
- Direction
- highere7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e7“However, when LIR:RepA-containing transgenic embryos were used in subsequent rounds of transformation, frequencies were higher in the RepA+ embryos”
The same questions in other papers
transgenic · cytology · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.