Abstract
The Arabidopsis thaliana genome encodes three RNA-binding proteins (RBPs), UBP1-associated protein 2a (UBA2a), UBA2b, and UBA2c, that contain two RNA-recognition motif (RRM) domains. They play important roles in wounding response and leaf senescence, and are homologs of Vicia faba abscisic-acid-activated protein kinase-interacting protein 1 (VfAKIP1). The potato (Solanum tuberosum) genome encodes at least seven AKIP1-like RBPs. Here, two potato RBPs have been characterized, StUBA2a/b and StUBA2c, that are homologous to VfAKIP1 and Arabidopsis UBA2s. Transient expression of StUBA2s induced a hypersensitive-like cell death phenotype in tobacco leaves, and an RRM-domain deletion assay of StUBA2s revealed that the first RRM domain is crucial for the phenotype. Unlike overexpression of Arabidopsis UBA2s, constitutive expression of StUBA2a/b in Arabidopsis did not cause growth arrest and lethality at the young seedling stage, but induced early leaf senescence. This phenotype was associated with increased expression of defence- and senescence-associated genes, including pathogen-related genes (PR) and a senescence-associated gene (SAG13), and it was aggravated upon flowering and ultimately resulted in a shortened life cycle. Leaf senescence of StUBA2a/b Arabidopsis plants was enhanced under darkness and was accompanied by H2O2 accumulation and altered expression of autophagy-associated genes, which likely cause cellular damage and are proximate causes of the early leaf senescence. Expression of salicylic acid signalling and biosynthetic genes was also upregulated in StUBA2a/b plants. Consistent with the localization of UBA2s-GFPs and VfAKIP1-GFP, soluble-modified GFP-StUBA2s localized in the nucleus within nuclear speckles. StUBA2s potentially can be considered for transgenic approaches to induce potato shoot senescence, which is desirable at harvest.
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Claims 13
Claim · model-written summary
Potato encodes at least seven AKIP1-like RNA-binding proteins.
- Taxon
- potato
Solanum tuberosume0 - Outcome
- AKIP1-like RBPse0
- Value
- at least sevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potato (Solanum tuberosum) genome encodes at least seven AKIP1-like RBPs.”
Claim · model-written summary
StUBA2a/b and StUBA2c are homologous to VfAKIP1 and Arabidopsis UBA2s.
- Taxon
- potatoSolanum tuberosume0
- Gene
- StUBA2a/b and StUBA2ce0
- Outcome
- homologous to VfAKIP1 and Arabidopsis UBA2se0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two potato RBPs have been characterized, StUBA2a/b and StUBA2c, that are homologous to VfAKIP1 and Arabidopsis UBA2s”
Claim · model-written summary
Transient StUBA2 expression induced hypersensitive-like cell death in tobacco leaves.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- leavese0
- Treatment
- Transient expression of StUBA2se0
- Gene
- StUBA2se0
- Outcome
- hypersensitive-like cell death phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of StUBA2s induced a hypersensitive-like cell death phenotype in tobacco leaves”
Claim · model-written summary
The first RRM domain was crucial for the cell death phenotype.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- StUBA2se0
- Outcome
- phenotypee0
- Qualifier
- first RRM domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an RRM-domain deletion assay of StUBA2s revealed that the first RRM domain is crucial for the phenotype”
e1“tobacco leaves”
Claim · model-written summary
Constitutive StUBA2a/b expression did not cause young-seedling growth arrest or lethality in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- young seedling stagee0
- Treatment
- constitutive expression of StUBA2a/be0
- Gene
- StUBA2a/be0
- Outcome
- growth arrest and lethalitye0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of StUBA2a/b in Arabidopsis did not cause growth arrest and lethality at the young seedling stage”
Claim · model-written summary
Constitutive StUBA2a/b expression induced early leaf senescence in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- constitutive expression of StUBA2a/be0
- Gene
- StUBA2a/be0
- Outcome
- early leaf senescencee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of StUBA2a/b in Arabidopsis did not cause growth arrest and lethality at the young seedling stage, but induced early leaf senescence”
Claim · model-written summary
Early senescence was associated with increased expression of defence and senescence genes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- pathogen-related genes (PR) and a senescence-associated gene (SAG13)e0
- Outcome
- expression of defence- and senescence-associated genese0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This phenotype was associated with increased expression of defence- and senescence-associated genes, including pathogen-related genes (PR) and a senescence-associated gene (SAG13)”
t0“Arabidopsis”
Claim · model-written summary
The phenotype worsened upon flowering and shortened the life cycle.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- floweringe0
- Outcome
- shortened life cyclee0
- Direction
- shortenede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was aggravated upon flowering and ultimately resulted in a shortened life cycle”
t0“Arabidopsis”
Claim · model-written summary
Darkness enhanced leaf senescence of StUBA2a/b Arabidopsis plants.
- Taxon
- ArabidopsisArabidopsise0
- Material
- StUBA2a/b Arabidopsis plantse0
- Treatment
- darknesse0
- Outcome
- Leaf senescencee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf senescence of StUBA2a/b Arabidopsis plants was enhanced under darkness”
Claim · model-written summary
Senescence was accompanied by H2O2 accumulation and altered autophagy gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- H2O2e0
- Outcome
- H2O2 accumulation and altered expression of autophagy-associated genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was accompanied by H2O2 accumulation and altered expression of autophagy-associated genes”
t0“Arabidopsis”
Claim · model-written summary
Salicylic acid signalling and biosynthetic genes were upregulated in StUBA2a/b plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- StUBA2a/b plantse0
- Outcome
- Expression of salicylic acid signalling and biosynthetic genese0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of salicylic acid signalling and biosynthetic genes was also upregulated in StUBA2a/b plants”
t0“Arabidopsis”
Claim · model-written summary
GFP-tagged StUBA2 proteins localized to nuclear speckles.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GFP-StUBA2se0
- Outcome
- localized in the nucleus within nuclear specklese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soluble-modified GFP-StUBA2s localized in the nucleus within nuclear speckles”
t0“Arabidopsis”
Claim · model-written summary
Authors propose StUBA2s for inducing potato shoot senescence at harvest.
- Taxon
- potatoSolanum tuberosume0
- Gene
- StUBA2se0
- Outcome
- transgenic approaches to induce potato shoot senescencee0
- Qualifier
- potentiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StUBA2s potentially can be considered for transgenic approaches to induce potato shoot senescence, which is desirable at harvest”
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.