Abstract
Salicylic acid (SA) and reactive oxygen species (ROS) are two well-defined inducers of leaf senescence. Here, we identified a novel WRKY transcription factor gene WSR1 (WRKY regulating SA and ROS 1) in Brassica napus (rapeseed) in promoting SA and ROS production, which eventually led to leaf senescence thereafter. Its expression increased in senescing leaves. Ca2+-dependent protein kinase (CPK) 5 and -6 interacted with and phosphorylated BnaWSR1. Overexpression of phosphomimic BnaWSR1 (BnaWSR1ca) in rapeseed protoplasts elicited ROS production and cell death while its ectopic expression in Arabidopsis enhanced SA and ROS levels and, hence, accelerated leaf senescence. Furthermore, BnaWSR1ca activated the expression of Isochorismate Synthase 1 (ICS1), Respiratory Burst Oxidase Homologue (Rboh) D, and Senescence-Associated Gene 14 (SAG14). Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) assays demonstrated that BnaWSR1ca directly bound to promoter regions of ICS1, RbohD, and SAG14. These data have identified a CPK-WSR1 module that integrates SA and ROS to control cell death and leaf senescence.
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Claims 8
Claim · model-written summary
CPK5 and CPK6 interact with and phosphorylate BnaWSR1.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Gene
- BnaWSR1e1
- Outcome
- interacted with and phosphorylated BnaWSR1e1
- Qualifier
- Ca2+-dependent protein kinase (CPK) 5 and -6e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“in Brassica napus (rapeseed)”
e1“Ca2+-dependent protein kinase (CPK) 5 and -6 interacted with and phosphorylated BnaWSR1”
Claim · model-written summary
WSR1 expression increased in senescing leaves.
- Taxon
- Brassica napusBrassica napuse0
- Organ / stage
- senescing leavese2
- Outcome
- expressione2
- Direction
- increasede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Brassica napus (rapeseed)”
e2“Its expression increased in senescing leaves”
Claim · model-written summary
Overexpression of phosphomimic BnaWSR1ca in rapeseed protoplasts elicited ROS production and cell death.
- Taxon
- rapeseede3
- Treatment
- Overexpression of phosphomimic BnaWSR1 (BnaWSR1ca)e3
- Outcome
- ROS production and cell deathe3
- Direction
- elicitede3
Not reportedComparator
Verbatim
e3“Overexpression of phosphomimic BnaWSR1 (BnaWSR1ca) in rapeseed protoplasts elicited ROS production and cell death”
Claim · model-written summary
Ectopic expression of WSR1 in Arabidopsis enhanced SA and ROS levels.
- Taxon
- Arabidopsis
Arabidopsise4 - Treatment
- ectopic expressione4
- Outcome
- SA and ROS levelse4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“while its ectopic expression in Arabidopsis enhanced SA and ROS levels and, hence, accelerated leaf senescence”
Claim · model-written summary
Ectopic expression of WSR1 in Arabidopsis accelerated leaf senescence.
- Taxon
- ArabidopsisArabidopsise4
- Treatment
- ectopic expressione4
- Outcome
- leaf senescencee5
- Direction
- acceleratede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“while its ectopic expression in Arabidopsis enhanced SA and ROS levels and, hence, accelerated leaf senescence”
e5“hence, accelerated leaf senescence”
Claim · model-written summary
BnaWSR1ca activated the expression of ICS1, RbohD and SAG14.
- Taxon
- Brassica napusBrassica napuse0
- Treatment
- BnaWSR1cae6
- Gene
- Isochorismate Synthase 1 (ICS1), Respiratory Burst Oxidase Homologue (Rboh) D, and Senescence-Associated Gene 14 (SAG14)e6
- Outcome
- expressione6
- Direction
- activatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Brassica napus (rapeseed)”
e6“BnaWSR1ca activated the expression of Isochorismate Synthase 1 (ICS1), Respiratory Burst Oxidase Homologue (Rboh) D, and Senescence-Associated Gene 14 (SAG14)”
Claim · model-written summary
BnaWSR1ca directly bound to the promoter regions of ICS1, RbohD and SAG14.
- Taxon
- Brassica napusBrassica napuse0
- Gene
- ICS1, RbohD, and SAG14e7
- Outcome
- directly bound to promoter regionse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Brassica napus (rapeseed)”
e7“BnaWSR1ca directly bound to promoter regions of ICS1, RbohD, and SAG14”
Claim · model-written summary
A CPK-WSR1 module integrates SA and ROS to control cell death and leaf senescence.
- Taxon
- Brassica napusBrassica napuse0
- Gene
- CPK-WSR1e1
- Compound
- SA and ROSe1
- Outcome
- control cell death and leaf senescencee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Brassica napus (rapeseed)”
e1“These data have identified a CPK-WSR1 module that integrates SA and ROS to control cell death and leaf senescence”
The same questions in other papers
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See all 11 papers →overexpression · physiology: oxidative · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 16 in the opposite direction
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- positive
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.