Abstract
Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach. The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves. However, biochemical analyses revealed that the transgenic lines displayed a considerable shift in metabolism, being characterized by decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H. The plants showed little change in photosynthesis with the exception of a minor decrease in maximum photosynthetic efficiency (F (v)/F (m)), and a small decrease in growth compared to the wild type. These results reveal that even small changes in cytosolic NADP-dependent isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acids. These data are discussed within the context of current models for the role of the various isoforms of isocitrate dehydrogenase within plant amino acid metabolism.
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Claims 6
Claim · model-written summary
Transgenic tomato leaves had mildly reduced SlICDH1 expression and enzyme activity.
- Taxon
- tomato
Solanum lycopersicumt0 - Organ / stage
- leavese0
- Treatment
- transformantse0
- Gene
- SlICDH1t0
- Outcome
- expression and activity of the target enzymee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves.”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
Claim · model-written summary
Transgenic lines had lower TCA cycle intermediates, total amino acids and photosynthetic pigments.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transgenic linest1
- Outcome
- TCA cycle intermediates, total amino acids, photosynthetic pigmentse0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
t1“the transgenic lines displayed a considerable shift in metabolism”
Claim · model-written summary
Transgenic lines had reduced starch and NAD(P)H.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transgenic linest1
- Outcome
- starch and NAD(P)He0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
t1“the transgenic lines displayed a considerable shift in metabolism”
Claim · model-written summary
Maximum photosynthetic efficiency decreased slightly.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transgenic linest1
- Outcome
- maximum photosynthetic efficiencye0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a minor decrease in maximum photosynthetic efficiency (F (v)/F (m))”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
t1“the transgenic lines displayed a considerable shift in metabolism”
Claim · model-written summary
Growth decreased slightly relative to wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transgenic linest1
- Outcome
- growthe0
- Direction
- decreasee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a small decrease in growth compared to the wild type”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
t1“the transgenic lines displayed a considerable shift in metabolism”
Claim · model-written summary
Small changes in enzyme activity altered enzymes of nitrate assimilation and amino acid synthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- cytosolic NADP-dependent isocitrate dehydrogenasee0
- Outcome
- activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“even small changes in cytosolic NADP-dependent isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acids”
t0“Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach.”
The same questions in other papers
transgenic · physiology: photosynthesis · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.