Paper · 2014 · Biotechnology in Animal Husbandry

Genetic variability of red clover seedlings in relation to salt stress

V. Mandic; V. Krnjaja; Z. Bijelic; et al.

10
claims
19
verbatim quotes
6
citations
2
research areas
✓
Open access · cc-by

Abstract

Red clover is highly salt-sensitive plant, especially during germination and early seedling growth stages. The aim of this investigation was to estimate the effects of different saline conditions (0, 50, 100, 150, and 200mM NaCl) on germination and early seedling growth in four red clover varieties (Kolubara, K-32, K-17 and K-39). Germination test was conducted in the laboratory conditions using sterile plastic vessels on filter paper moistened with 10ml of the appropriate salt test solutions. It was observed that the germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium. The tested varieties of red clover showed different NaCl tolerance at the seedling stage. Generally, studied red clover varieties are very sensitive to salt, especially K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS. Variety K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest. Testing of varieties of red clover in the early seedling growth at different concentrations of NaCl in the growing medium could be helpful in the identification and selection of varieties for cultivation on saline soils.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 10

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing NaCl significantly decreased germination energy and germination.

Taxon
red cloverTrifolium pratenset0
Treatment
increasing concentrations of NaCle0
Outcome
germination energy (GE), germination (G)e0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium”

✓ located verbatim in the source · abstract, characters 524–841 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Intervention effectobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

Increasing NaCl significantly decreased dead or infected seed percentage and normal seedlings.

Taxon
red cloverTrifolium pratenset0
Treatment
increasing concentrations of NaCle0
Outcome
percentage of dead or infected seeds (DIS), normal seedlings (NS)e0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium”

✓ located verbatim in the source · abstract, characters 566–841 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing NaCl significantly decreased root length, shoot length, and seedling fresh weight.

Taxon
red cloverTrifolium pratenset0
Treatment
increasing concentrations of NaCle0
Outcome
root length (RL), shoot length (ShL), fresh weight (FW)e0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium”

✓ located verbatim in the source · abstract, characters 633–841 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing NaCl significantly decreased seedling dry weight and vigor index.

Taxon
red cloverTrifolium pratenset0
Treatment
increasing concentrations of NaCle0
Outcome
dry weight of seedling (DW) and seedling vigor index (SVI)e0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium”

✓ located verbatim in the source · abstract, characters 693–841 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Red clover varieties differed in seedling-stage NaCl tolerance.

Taxon
red cloverTrifolium pratensee0
Organ / stage
seedling stagee0
Outcome
NaCl tolerancee0
Direction
differente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The tested varieties of red clover showed different NaCl tolerance at the seedling stage”

✓ located verbatim in the source · abstract, characters 843–931 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

K-32 had the lowest germination energy, germination and normal seedling values.

Taxon
red cloverTrifolium pratenset0
Material
K-32e0
Outcome
GE, G, NSe0
Direction
loweste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS”

✓ located verbatim in the source · abstract, characters 1012–1086 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

K-32 had the lowest seedling vigor index.

Taxon
red cloverTrifolium pratenset0
Material
K-32e0
Outcome
SVIe0
Direction
loweste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS”

✓ located verbatim in the source · abstract, characters 1012–1086 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

K-32 had the highest dead or infected seed percentage.

Taxon
red cloverTrifolium pratenset0
Material
K-32e0
Outcome
DISe0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS”

✓ located verbatim in the source · abstract, characters 1012–1086 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

K-17 had the highest germination energy, germination and normal seedlings under salt stress.

Taxon
red cloverTrifolium pratenset0
Material
K-17e0
Outcome
GE, G, NSe0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest”

✓ located verbatim in the source · abstract, characters 1096–1231 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

K-17 had the highest root length and seedling vigor index under salt stress.

Taxon
red cloverTrifolium pratenset0
Material
K-17e0
Outcome
RL and SVIe0
Direction
higheste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest”

✓ located verbatim in the source · abstract, characters 1096–1231 · show in text ↑
t0

“red clover”

✓ located verbatim in the source · title, characters 23–33 · show in text ↑

sodium chloride · growth · Trifolium pratense (red clover)Results agree

6 papers: 0 positive, 6 negative or no effect

5 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.