Abstract
Three Agrobacteriumrhizogenes strains (A4, 8196, and ATC39207) and two Agrobacteriumtumefaciens mutant strains (A208 (pTiT37 • 14a/a) and C58 (pGV3297)) were evaluated for their genetic transformation frequency invitro on cuttings of Populusdeltoides × nigra line DN106 and Populusnigra × maximowiczii lines NM1 and NM6. The aim of this work was to increase biomass production by genetically modifying hybrid poplar root systems. Inoculation of the cut end of invitro grown stems with the A. rhizogenes strain ATC39207 gave the highest frequency of typical hairy root formation with line DN106. The other combinations of Agrobacterium strains and poplar lines yielded very low frequencies of hairy root induction or induction of tumours instead of hairy roots. Similar attempts with greenhouse-grown plants gave negative results. The modified root system resulting from the invitro inoculation of line DN106 cuttings yielded an increase in root and aerial biomass production after 175 days of growth invitro. Preliminary data confirm this observation in the greenhouse. Genetic transformation of the tissues was proven by phenotype (hairy root or tumour), hormone autotrophy, and opine production in the resulting tissues. Because of the host range restriction and inefficiency of this approach, it is not practical at this time for use in the transformation of hybrid poplar.
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Claims 6
Claim · model-written summary
Inoculation of the cut end of in vitro stems with Agrobacterium rhizogenes strain ATC39207 gave the highest frequency of typical hairy root formation in line DN106.
- Material
- line DN106e1
- Treatment
- Inoculation of the cut end of invitro grown stemse1
- Interactor
- A. rhizogenes strain ATC39207e1other
- Outcome
- frequency of typical hairy root formatione1
- Direction
- higheste1
- Comparator
- other combinations of Agrobacterium strains and poplar linese2
- Setting
- invitroe1
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e1“Inoculation of the cut end of invitro grown stems with the A. rhizogenes strain ATC39207 gave the highest frequency of typical hairy root formation with line DN106”
e2“The other combinations of Agrobacterium strains and poplar lines yielded very low frequencies of hairy root induction or induction of tumours instead of hairy roots”
Claim · model-written summary
Other combinations of Agrobacterium strains and poplar lines gave very low frequencies of hairy root induction, or tumours instead of hairy roots.
- Treatment
- other combinations of Agrobacterium strains and poplar linese2
- Outcome
- frequencies of hairy root inductione2
- Direction
- very lowe2
- Qualifier
- or induction of tumours instead of hairy rootse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e2“The other combinations of Agrobacterium strains and poplar lines yielded very low frequencies of hairy root induction or induction of tumours instead of hairy roots”
Claim · model-written summary
Similar transformation attempts on greenhouse-grown hybrid poplar plants gave negative results.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e3“Similar attempts with greenhouse-grown plants gave negative results”
Claim · model-written summary
Inoculation of line DN106 cuttings in vitro increased root and aerial biomass production after 175 days of growth.
- Material
- line DN106e4
- Treatment
- invitro inoculatione4
- Outcome
- root and aerial biomass productione4
- Direction
- an increasee4
- Qualifier
- after 175 days of growthe4
- Setting
- invitroe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e4“The modified root system resulting from the invitro inoculation of line DN106 cuttings yielded an increase in root and aerial biomass production after 175 days of growth invitro”
Claim · model-written summary
Genetic transformation of the hybrid poplar tissues was proven by hairy-root or tumour phenotype, hormone autotrophy and opine production.
- Outcome
- Genetic transformation of the tissuese5
- Qualifier
- by phenotype (hairy root or tumour), hormone autotrophy, and opine productione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e5“Genetic transformation of the tissues was proven by phenotype (hairy root or tumour), hormone autotrophy, and opine production in the resulting tissues”
Claim · model-written summary
The authors conclude that this Agrobacterium approach is not practical for transforming hybrid poplar at this time.
- Outcome
- use in the transformation of hybrid poplare6
- Qualifier
- Because of the host range restriction and inefficiency of this approache6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e6“Because of the host range restriction and inefficiency of this approach, it is not practical at this time for use in the transformation of hybrid poplar”
The same questions in other papers
inoculation · yield · Populus (poplar)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.