Abstract
Tomato yellow leaf curl virus (TYLCV) is a DNA virus belonging to the genus Begomovirus. TYLCV replicates using double-stranded DNA intermediates that can become the target of plant transcriptional gene silencing (TGS). Here, we show that the V2 protein of TYLCV can suppress TGS of a transcriptionally silenced green fluorescent protein (GFP) transgene in Nicotiana benthamiana line 16-TGS. Through bisulfite sequencing and chop-PCR, we demonstrated that the TYLCV V2 can reverse GFP transgene silencing by reducing the methylation levels in the 35S promoter sequence. Both AtSN1 and MEA-ISR loci in Arabidopsis thaliana were previously reported to be strongly methylated, and we show that the methylation status of both loci was significantly reduced in TYLCV V2 transgenic Arabidopsis plants. We conclude that TYLCV can efficiently suppress TGS when it infects plants, and its V2 protein is responsible for the TGS suppression activity.
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Claims 4
Claim · model-written summary
TYLCV V2 suppressed silencing of a GFP transgene in Nicotiana benthamiana line 16-TGS.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Material
- line 16-TGSe0
- Treatment
- V2 protein of TYLCVe0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- green fluorescent protein (GFP)e0
- Outcome
- TGS of a transcriptionally silenced green fluorescent protein (GFP) transgenee0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the V2 protein of TYLCV can suppress TGS of a transcriptionally silenced green fluorescent protein (GFP) transgene in Nicotiana benthamiana line 16-TGS”
Claim · model-written summary
TYLCV V2 reversed GFP transgene silencing by reducing methylation of the 35S promoter.
- Taxon
- plantsViridiplantaet0
- Treatment
- TYLCV V2e0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- GFPe0
- Outcome
- GFP transgene silencinge0
- Direction
- reversee0
- Qualifier
- reducing the methylation levels in the 35S promoter sequencee0
Verbatim
e0“the TYLCV V2 can reverse GFP transgene silencing by reducing the methylation levels in the 35S promoter sequence”
t0“in plants”
Claim · model-written summary
TYLCV V2 transgenic Arabidopsis plants had significantly reduced methylation at AtSN1 and MEA-ISR loci.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Material
- TYLCV V2 transgenic Arabidopsis plantse0
- Treatment
- TYLCV V2 transgenic Arabidopsis plantse0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- AtSN1 and MEA-ISR locie1
- Outcome
- methylation status of both locie0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the methylation status of both loci was significantly reduced in TYLCV V2 transgenic Arabidopsis plants”
e1“Both AtSN1 and MEA-ISR loci in Arabidopsis thaliana were previously reported to be strongly methylated”
t0“in plants”
Claim · model-written summary
The authors concluded that TYLCV suppresses plant TGS during infection through its V2 protein.
- Taxon
- plantsViridiplantaee0
- Interactor
- TYLCVTomato yellow leaf curl viruse0pathogen
- Gene
- V2 proteine0
- Outcome
- TGSe0
- Direction
- suppresse0
Verbatim
e0“TYLCV can efficiently suppress TGS when it infects plants, and its V2 protein is responsible for the TGS suppression activity”
The same questions in other papers
Solanum lycopersicum · Tomato yellow leaf curl virus · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.