Abstract
The higher plant tumors are convenient models for studying the genetic control mechanism of plant cell division. There are two types of tumors: induced by the pathogenic factor and genetically determined. The development of both tumor types was related to the changes in cytokinin metabolism and/or signal transduction. In this work, the effect of synthetic cytokinins on the in vitro morphogenesis of cotyledon explants and isolated apices of radish seedlings was studied in several inbred radish lines ( Raphanus sativus var. radicula Pers.) that differed in their in vivo tumorigenic properties. It was noted that root formation was stronger affected by kinetin while the treatment with thidiazuron tended to induce active callus formation in cotyledon explants of all inbred lines, except IIa . Growing with benzyladenine produced an intermediate effect as regards all morphogenetic responses. Cytokinin treatment of tumorigenic lines enhanced necrotic development in cotyledon explants. Culturing isolated apices of regenerated plants produced tumors anatomically and morphologically similar to those developing in vivo. Some of the lines nontumorigenic in vivo with enhanced formation of calli on cotyledon explants also developed tumors on apical explants in vitro when treated with cytokinins. These data suggest that different mechanisms for tumor formation operate in various radish lines. The radish lines are classified into three types: (1) necrotic lines with tumor formation putatively related to endogenous cytokinin level, (2) callus-forming lines with cell division enhanced in response to cytokinins, and (3) necrosis-and callus-forming lines with both mechanisms of tumor formation involved.
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Claims 7
Claim · model-written summary
Kinetin had a stronger effect on root formation in radish explants.
- Taxon
- Raphanus sativus var. radicula Pers.
.jpg?width=60)
Raphanus sativus var. sativust0 - Treatment
- kinetine0
- Outcome
- root formatione0
- Direction
- stronger affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“root formation was stronger affected by kinetin”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
Thidiazuron tended to induce active callus formation in cotyledon explants of all lines except IIa.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Organ / stage
- cotyledon explantse0
- Treatment
- thidiazurone0
- Outcome
- callus formatione0
- Direction
- inducee0
- Qualifier
- except IIae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with thidiazuron tended to induce active callus formation in cotyledon explants of all inbred lines, except IIa”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
Benzyladenine produced an intermediate effect on radish morphogenetic responses.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Treatment
- benzyladeninee0
- Outcome
- morphogenetic responsese0
- Direction
- intermediate effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growing with benzyladenine produced an intermediate effect as regards all morphogenetic responses.”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
Cytokinin treatment enhanced necrotic development in cotyledon explants of tumorigenic radish lines.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Organ / stage
- cotyledon explantse0
- Treatment
- Cytokinin treatmente0
- Outcome
- necrotic developmente0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytokinin treatment of tumorigenic lines enhanced necrotic development in cotyledon explants.”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
Cultured isolated apices of regenerated radish plants produced tumors resembling those formed in vivo.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Organ / stage
- isolated apicese0
- Outcome
- tumorse0
- Comparator
- those developing in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culturing isolated apices of regenerated plants produced tumors anatomically and morphologically similar to those developing in vivo.”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
Some lines without in vivo tumors developed tumors on apical explants after cytokinin treatment in vitro.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Organ / stage
- apical explantse0
- Treatment
- cytokininse0
- Outcome
- tumorse0
- Direction
- developede0
- Qualifier
- nontumorigenic in vivoe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of the lines nontumorigenic in vivo with enhanced formation of calli on cotyledon explants also developed tumors on apical explants in vitro when treated with cytokinins.”
t0“Raphanus sativus var. radicula Pers.”
Claim · model-written summary
The radish lines were classified into necrotic, callus-forming, and combined types of tumor formation.
- Taxon
- Raphanus sativus var. radicula Pers.Raphanus sativus var. sativust0
- Outcome
- three typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The radish lines are classified into three types: (1) necrotic lines with tumor formation putatively related to endogenous cytokinin level, (2) callus-forming lines with cell division enhanced in response to cytokinins, and (3) necrosis-and callus-forming lines with both mechanisms of tumor formation involved.”
t0“Raphanus sativus var. radicula Pers.”
The same questions in other papers
class · growth · Raphanus sativus (radish)Positive and negative results
2 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.