Abstract
As water and nutrient uptake should be related in the response of plants to salinity, the aim of this paper is to establish whether or not aquaporin functionality is related to H+-ATPase activity in root cells of pepper (Capsicum annuum L.) plants. Thus, H+-ATPase activity was measured in plasma membrane vesicles isolated from roots and aquaporin functionality was measured using a cell pressure probe in intact roots. Salinity was applied as 60 mM NaCl or 60 mM KCl, to determine which ion (Na+, K+ or Cl-) is producing the effects. We also investigated whether the effects of both salts were ameliorated by Ca2+. Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl and an ameliorative effect of Ca2+. However, fusicoccin (an activator of H+-ATPase) did not alter osmotic water permeability of protoplasts isolated from roots. Addition of Hg2+ inhibited both ATPase and aquaporins, but ATPase also contains Hg-binding sites. Therefore, the results indicate that H+-ATPase and aquaporin activities may not be related in pepper plants.
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Claims 7
Claim · model-written summary
NaCl reduced root cell hydraulic conductivity and ATPase activity.
- Taxon
- peppere1
- Treatment
- NaCle0
- Gene
- H+-ATPasee0
- Outcome
- cell hydraulic conductivity, Lpc, and H+-ATPase activitye0
- Direction
- large reductionse0
Not reportedComparator
Verbatim
e0“Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
KCl reduced root cell hydraulic conductivity and ATPase activity.
- Taxon
- peppere1
- Treatment
- KCle0
- Gene
- H+-ATPasee0
- Outcome
- cell hydraulic conductivity, Lpc, and H+-ATPase activitye0
- Direction
- large reductionse0
Not reportedComparator
Verbatim
e0“Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
Calcium ameliorated salt effects on hydraulic conductivity and ATPase activity.
Verbatim
e0“Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl and an ameliorative effect of Ca2+.”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
Fusicoccin did not alter protoplast osmotic water permeability.
- Taxon
- peppere1
- Organ / stage
- rootse0
- Treatment
- fusicoccine0
- Outcome
- osmotic water permeabilitye0
- Direction
- did not altere0
Not reportedComparator
Verbatim
e0“fusicoccin (an activator of H+-ATPase) did not alter osmotic water permeability of protoplasts isolated from roots.”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
Mercury inhibited both ATPase and aquaporins.
Not reportedComparator
Verbatim
e0“Addition of Hg2+ inhibited both ATPase and aquaporins”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
ATPase also contains mercury binding sites.
Verbatim
e0“ATPase also contains Hg-binding sites.”
e1“pepper (Capsicum annuum L.) plants”
Claim · model-written summary
The authors infer ATPase and aquaporin activities may not be related in pepper.
Not reportedDirection
Verbatim
e0“H+-ATPase and aquaporin activities may not be related in pepper plants.”