Abstract
To test the hypothesis that the carrier-mediated component of the indoleacetic acid (IAA) influx involves an electrogenic proton/IAA anion symport, the effects on the IAA influx of salts expected to depolarize the membrane potential were examined in suspension-cultured soybean (Glycine max [L.] Merr.) root cells. Although KCl does inhibit carrier-mediated uptake, the effect is specific to the anion at low concentrations and not due to more general processes such as changes in ionic or osmotic strength. Other anions such as bromide, iodide, and fluoride inhibit the carrier more strongly. Because potassium iminodiacetate, which is also expected to depolarize the membrane potential, has no inhibitory effect on the IAA influx, there is no evidence for the involvement of the membrane potential in carrier-mediated uptake. It is therefore most likely that in soybean cells, if carrier-mediated uptake occurs via a proton symport, the H(+):IAA- stoichiometry is 1:1. At concentrations greater than 70 millimolar, sorbitol, a nonionic osmoticum, inhibits carrier-mediated IAA uptake. The effects of specific anions and osmotic potential on the uptake carrier necessitates the reevaluation of other auxin transport studies in which KCl was routinely used as an agent with which to depolarize the membrane potential.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
KCl inhibited carrier-mediated IAA uptake through an anion-specific effect at low concentrations.
- Taxon
- soybean
Glycine maxt0 - Treatment
- KCle0
- Outcome
- carrier-mediated uptakee0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although KCl does inhibit carrier-mediated uptake, the effect is specific to the anion at low concentrations and not due to more general processes such as changes in ionic or osmotic strength.”
t0“Soybean Root Cells”
Claim · model-written summary
Bromide, iodide and fluoride inhibited the uptake carrier more strongly.
- Taxon
- soybeanGlycine maxt0
- Treatment
- bromide, iodide, and fluoridee0
- Outcome
- carriere0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other anions such as bromide, iodide, and fluoride inhibit the carrier more strongly.”
t0“Soybean Root Cells”
Claim · model-written summary
Potassium iminodiacetate did not inhibit IAA influx.
- Taxon
- soybeanGlycine maxt0
- Treatment
- potassium iminodiacetatee0
- Outcome
- IAA influxe0
- Direction
- no inhibitory effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potassium iminodiacetate, which is also expected to depolarize the membrane potential, has no inhibitory effect on the IAA influx”
t0“Soybean Root Cells”
Claim · model-written summary
Sorbitol above 70 millimolar inhibited carrier-mediated IAA uptake.
- Taxon
- soybeanGlycine maxt0
- Treatment
- sorbitole0
- Outcome
- carrier-mediated IAA uptakee0
- Direction
- inhibitse0
- Qualifier
- greater than 70 millimolare0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At concentrations greater than 70 millimolar, sorbitol, a nonionic osmoticum, inhibits carrier-mediated IAA uptake.”
t0“Soybean Root Cells”
The same questions in other papers
potassium chloride · nutrient: content · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- no effect
4 papers more in this group
See all 8 papers →glucitol · nutrient: content · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
potassium · molecular: metabolites · Glycine max (soybean)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.