Abstract
Double-stranded cDNA was synthesized from polysomal poly(A)-containing RNA of illuminated barley plants and was inserted into the PstI site of the bacterial plasmid pBR322. Two different strategies were used for the screening of the bacterial colonies. Light-regulated sequences were detected by differential hybridization with cDNA of polyadenylated RNA from dark-grown and illuminated barley plants. For a second screening step partially purified mRNAs encoding the light-harvesting chlorophyll a/b protein were used for the synthesis of another cDNA probe. By using these procedures a cDNA clone was isolated which encodes a constitutive polypeptide of the light-harvesting chlorophyll a/b protein. This cloned cDNA has been used to assess the effect of phytochrome on the steady-state level of mRNA sequences encoding the light-harvesting chlorophyll a/b protein. The mRNA is almost undetectable in dark-grown plants. Following treatment with red light, the concentration of this mRNA sequence increases rapidly during the subsequent dark period. This red light effect can be reversed substantially by irradiation with far-red light. These results indicate that not only the amount of mRNA activity as shown previously, but also the steady-state level of mRNA sequences encoding the light-harvesting chlorophyll a/b is controlled by phytochrome.
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Claims 5
Claim · model-written summary
A cDNA clone encoding a constitutive light harvesting chlorophyll protein polypeptide was isolated.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Gene
- light-harvesting chlorophyll a/b proteine0
- Outcome
- cDNA clonee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a cDNA clone was isolated which encodes a constitutive polypeptide of the light-harvesting chlorophyll a/b protein.”
t0“Barley”
Claim · model-written summary
The light harvesting protein mRNA was almost undetectable in dark grown barley.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- mRNAe0
- Direction
- almost undetectablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNA is almost undetectable in dark-grown plants.”
t0“Barley”
Claim · model-written summary
Red light rapidly increased the light harvesting protein mRNA during the subsequent dark period.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- red lighte0
- Outcome
- concentration of this mRNA sequencee0
- Direction
- increases rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Following treatment with red light, the concentration of this mRNA sequence increases rapidly during the subsequent dark period.”
t0“Barley”
Claim · model-written summary
Far red irradiation substantially reversed the red light effect on the mRNA.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- irradiation with far-red lighte0
- Outcome
- red light effecte0
- Direction
- reversed substantiallye0
- Comparator
- red light effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This red light effect can be reversed substantially by irradiation with far-red light.”
t0“Barley”
Claim · model-written summary
The authors interpret steady state light harvesting mRNA levels as phytochrome controlled.
- Taxon
- barleyHordeum vulgaret0
- Gene
- light-harvesting chlorophyll a/be0
- Outcome
- steady-state level of mRNA sequencese0
- Qualifier
- controlled by phytochromee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the steady-state level of mRNA sequences encoding the light-harvesting chlorophyll a/b is controlled by phytochrome.”
t0“Barley”
The same questions in other papers
light · molecular: expression · Hordeum vulgare (barley)Positive and negative results
19 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
- no effect
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.