Abstract
Treatment of six-day-old barley leaves with white light of high intensity, 250-2000 W/m2, leads to a linear increase in the steady-state concentrations of early light-inducible protein (ELIP) mRNA followed by an accumulation of the protein. Accumulation of ELIP mRNA, under light stress, is highest in the basal third of the leaf and declines to approximately 50% of this level in the apical segment. The amount of the accumulated protein decreases more steeply towards the tip than would be expected from mRNA levels. This finding, as well as the fact that during greening a massive accumulation of the protein starts only at a time when the steady-state concentrations of ELIP mRNA have declined to 10% of the maximal value, indicate post-transcriptional control. Accumulation is presumably achieved by stabilization of the protein. ELIP mRNA and protein levels, induced by a 2-h period of high-light stress, are lowest in the afternoon and highest at midnight and during the morning. The inducibility of ELIP by high light is therefore under diurnal control. An increase of light stress, due to application of the carotenoid-biosynthesis inhibitor norfluorazon, results in a considerable induction of ELIP mRNA and protein. The plant hormone abscisic acid exerts only a small effect on the mRNA level. In all cases studied, the light-induced increase in the amount of ELIP mRNA was accompanied by a corresponding decline in the mRNA levels for the apoprotein of the chlorophyll-a/b-binding protein. Steady-state concentrations of mRNA for the small subunit of ribulose-1,5-bisphosphate carboxylase were hardly affected under all investigated light intensities.
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Claims 12
Claim · model-written summary
High white light increased ELIP mRNA linearly and was followed by protein accumulation.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- white light of high intensity, 250-2000 W/m2e0
- Gene
- early light-inducible protein (ELIP)e0
- Outcome
- steady-state concentrations of early light-inducible protein (ELIP) mRNAe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Treatment of six-day-old barley leaves with white light of high intensity, 250-2000 W/m2, leads to a linear increase in the steady-state concentrations of early light-inducible protein (ELIP) mRNA followed by an accumulation of the protein”
Claim · model-written summary
ELIP mRNA was highest at the leaf base and declined to about half that at the apex.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- leafe0
- Gene
- ELIPe0
- Outcome
- Accumulation of ELIP mRNAe0
- Direction
- declinese0
- Value
- approximately 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of ELIP mRNA, under light stress, is highest in the basal third of the leaf and declines to approximately 50% of this level in the apical segment”
t0“barley”
Claim · model-written summary
ELIP protein declined toward the leaf tip more steeply than mRNA.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- amount of the accumulated proteine0
- Direction
- decreasese0
- Comparator
- mRNA levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amount of the accumulated protein decreases more steeply towards the tip than would be expected from mRNA levels”
t0“barley”
Claim · model-written summary
During greening, strong ELIP protein accumulation began after mRNA fell to ten percent of maximum.
- Taxon
- barleyHordeum vulgaret0
- Gene
- ELIPe0
- Outcome
- steady-state concentrations of ELIP mRNAe0
- Direction
- declinede0
- Value
- 10%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“during greening a massive accumulation of the protein starts only at a time when the steady-state concentrations of ELIP mRNA have declined to 10% of the maximal value”
t0“barley”
Claim · model-written summary
The mismatch between mRNA and protein indicates post-transcriptional control.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- post-transcriptional controle0
- Qualifier
- post-transcriptional controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicate post-transcriptional control”
t0“barley”
Claim · model-written summary
ELIP protein accumulation is presumed to involve protein stabilization.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- Accumulatione0
- Qualifier
- stabilization of the proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation is presumably achieved by stabilization of the protein”
t0“barley”
Claim · model-written summary
Light induced ELIP mRNA and protein were lowest in afternoon and highest at midnight and morning.
- Taxon
- barleyHordeum vulgaret0
- Gene
- ELIPe0
- Outcome
- ELIP mRNA and protein levelse0
- Direction
- loweste0
- Qualifier
- induced by a 2-h period of high-light stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ELIP mRNA and protein levels, induced by a 2-h period of high-light stress, are lowest in the afternoon and highest at midnight and during the morning”
t0“barley”
Claim · model-written summary
High light inducibility of ELIP is under diurnal control.
- Taxon
- barleyHordeum vulgaret0
- Gene
- ELIPe0
- Outcome
- inducibility of ELIPe0
- Qualifier
- diurnal controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inducibility of ELIP by high light is therefore under diurnal control”
t0“barley”
Claim · model-written summary
Norfluorazon strongly induced ELIP mRNA and protein.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- norfluorazone0
- Gene
- ELIPe0
- Outcome
- ELIP mRNA and proteine0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of the carotenoid-biosynthesis inhibitor norfluorazon, results in a considerable induction of ELIP mRNA and protein”
t0“barley”
Claim · model-written summary
Abscisic acid had only a small effect on ELIP mRNA.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- abscisic acide0
- Outcome
- mRNA levele0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plant hormone abscisic acid exerts only a small effect on the mRNA level”
t0“barley”
Claim · model-written summary
Increasing ELIP mRNA accompanied declining chlorophyll binding protein mRNA.
- Taxon
- barleyHordeum vulgaret0
- Gene
- apoprotein of the chlorophyll-a/b-binding proteine0
- Outcome
- mRNA levels for the apoprotein of the chlorophyll-a/b-binding proteine0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the light-induced increase in the amount of ELIP mRNA was accompanied by a corresponding decline in the mRNA levels for the apoprotein of the chlorophyll-a/b-binding protein”
t0“barley”
Claim · model-written summary
mRNA for the small Rubisco subunit was hardly affected across light intensities.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- light intensitiese0
- Gene
- small subunit of ribulose-1,5-bisphosphate carboxylasee0
- Outcome
- Steady-state concentrations of mRNA for the small subunit of ribulose-1,5-bisphosphate carboxylasee0
- Direction
- hardly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Steady-state concentrations of mRNA for the small subunit of ribulose-1,5-bisphosphate carboxylase were hardly affected under all investigated light intensities”
t0“barley”
The same questions in other papers
light · quality: protein · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
light · molecular: expression · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.