Paper · 1967 · PLANT PHYSIOLOGY

Isolation of Phytochrome from the Alga Mesotaenium and Liverwort Sphaerocarpos

A. O. Taylor; Bruce A. Bonner

5
claims
8
verbatim quotes
75
citations
1
research areas
✓
Open access

Abstract

Phytochrome has been isolated from the green alga Mesotaenium and the liverwort Sphaerocarpos. The Mesotaenium pigment had absorption peaks at 649 and 710 nm for the P(R) and P(FR) forms, respectively. Corresponding difference spectrum maxima for the Sphaerocarpos pigment were at 655 and 720 nm. While the absorption maxima differ, the reversibility and efficiency with which red and far-red light transform the Mesotaenium pigment are very similar to that reported for phytochrome isolated from etiolated seedlings of higher plants. Methods are described which allow efficient separation of phytochrome from highly pigmented light-grown material.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

The Mesotaenium pigment had absorption peaks at 649 and 710 nm for the Pr and Pfr forms.

Taxon
MesotaeniumMesotaeniume1
Outcome
absorption peakse1
Value
649 and 710 nme1
Qualifier
for the P(R) and P(FR) formse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mesotaenium
Mesotaenium · TaxonPeter de Lange · CC0 · Wikimedia Commons ↗

Verbatim

e1

“The Mesotaenium pigment had absorption peaks at 649 and 710 nm for the P(R) and P(FR) forms, respectively”

✓ located verbatim in the source · abstract, characters 95–200 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The Sphaerocarpos pigment had difference spectrum maxima at 655 and 720 nm.

Taxon
SphaerocarposSphaerocarpose2
Outcome
difference spectrum maximae2
Value
655 and 720 nme2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sphaerocarpos
Sphaerocarpos · Taxon · picture of the genus (one of its species)EncycloPetey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Liverwort Sphaerocarpos”

✓ located verbatim in the source · title, characters 55–78 · show in text ↑
e2

“Corresponding difference spectrum maxima for the Sphaerocarpos pigment were at 655 and 720 nm”

✓ located verbatim in the source · abstract, characters 202–295 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Red and far-red light transformed the Mesotaenium pigment with reversibility and efficiency very similar to phytochrome from etiolated higher-plant seedlings.

Taxon
MesotaeniumMesotaeniume3
Treatment
red and far-red lighte3
Outcome
reversibility and efficiencye3
Direction
very similare3
Comparator
phytochrome isolated from etiolated seedlings of higher plantse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mesotaenium
Mesotaenium · TaxonPeter de Lange · CC0 · Wikimedia Commons ↗

Verbatim

e3

“While the absorption maxima differ, the reversibility and efficiency with which red and far-red light transform the Mesotaenium pigment are very similar to that reported for phytochrome isolated from etiolated seedlings of higher plants”

✓ located verbatim in the source · abstract, characters 297–533 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The absorption maxima of the Sphaerocarpos and Mesotaenium pigments differ.

Taxon
SphaerocarposSphaerocarpost0
Outcome
absorption maximae3
Direction
differe3
Comparator
Mesotaenium pigmente3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sphaerocarpos
Sphaerocarpos · Taxon · picture of the genus (one of its species)EncycloPetey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Liverwort Sphaerocarpos”

✓ located verbatim in the source · title, characters 55–78 · show in text ↑
e3

“While the absorption maxima differ, the reversibility and efficiency with which red and far-red light transform the Mesotaenium pigment are very similar to that reported for phytochrome isolated from etiolated seedlings of higher plants”

✓ located verbatim in the source · abstract, characters 297–533 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The described methods allow efficient separation of phytochrome from highly pigmented light-grown Sphaerocarpos material.

Taxon
SphaerocarposSphaerocarpost0
Outcome
efficient separation of phytochromee4
Qualifier
from highly pigmented light-grown materiale4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sphaerocarpos
Sphaerocarpos · Taxon · picture of the genus (one of its species)EncycloPetey · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Liverwort Sphaerocarpos”

✓ located verbatim in the source · title, characters 55–78 · show in text ↑
e4

“Methods are described which allow efficient separation of phytochrome from highly pigmented light-grown material”

✓ located verbatim in the source · abstract, characters 535–647 · show in text ↑