Abstract
During the spring of 2007, pea plants (Pisum sativum L.) (cvs. Utrillo and Floreta) showing virus-like symptoms were observed in several commercial fields in the southern and eastern regions of Catalonia, Spain. Incidence of symptomatic plants ranged from 5 to 15% and was distributed in both small and large patches. Infected plants exhibited yellow mosaic leaf symptoms that later became translucent. Leaves gradually curled and in some cases developed enations near the veins on the abaxial surface. Plants were "bushy" and had shortened internodes. Infection prior to pod formation resulted in pods that were distorted and stunted (1). The infected leaves and pods were tested by indirect-ELISA with a potyvirus-specific antibody (Agdia, Elkhart, IN) and double-antibody sandwich (DAS)-ELISA with antibodies specific to Pea enation mosaic virus (PEMV), Broad bean wilt virus 1 (BBWV-1), Beet western yellow virus (BWYV), Bean yellow mosaic virus (BYMV), Alfalfa mosaic virus (AMV), and Tomato spotted wilt virus (TSWV) (Loewe Biochemica GmbH, Sauerlach, Germany). PEMV was detected in all 24 symptomatic samples that were collected from 10 locations between March 2007 and March 2008. Thirteen of these samples also tested positive for BWYV, but no differences in symptom expression were observed in plants infected with both viruses or PEMV alone. PEMV was also identified in seven broad bean plants (Vicia faba L.) from three additional locations. These plants expressed interveinal yellow mosaic on leaves and deformed pods. The genomic sequence of PEMV-1 (GenBank Accession No. L04573) was used to design primers to amplify a 451-nt segment of the polymerase gene by reverse transcription (RT)-PCR; PEMV-D (5'-TGACCATGAGTCCACTGAGG-3'), PEMV-R (5'-AGTATCTTCCAACAACCACAT-3'). One ELISA-positive sample was analyzed and the expected size amplicon was generated. Direct sequencing (GenBank Accession No. EU652339) revealed that PEMV-1 and our pea isolate have nucleotide sequence identities of 95%. To our knowledge, this is the first report of PEMV in Spain, which might cause important economical losses since PEMV is an important viral disease of pea and other legumes worldwide. Reference: (1) J. S. Skaf and G. A. Zoeten. No. 372 (No. 257 revised) in: Description of Plant Viruses. AAB, Kew, Surrey, England, 2000.
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Claims 9
Claim · model-written summary
PEMV was detected in all 24 symptomatic pea samples collected from 10 locations.
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Interactor
- PEMVe1pathogen
- Outcome
- detected in all 24 symptomatic samplese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea plants (Pisum sativum L.)”
e1“PEMV was detected in all 24 symptomatic samples that were collected from 10 locations between March 2007 and March 2008”
Claim · model-written summary
PEMV was identified in seven broad bean plants from three additional locations.
- Taxon
- Vicia faba
Vicia fabae2 - Interactor
- PEMVe2pathogen
- Outcome
- identified in seven broad bean plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PEMV was also identified in seven broad bean plants (Vicia faba L.) from three additional locations”
Claim · model-written summary
Thirteen of the PEMV-positive pea samples also tested positive for BWYV.
- Taxon
- PeaLathyrus oleraceust0
- Interactor
- BWYVBeet western yellows viruse3pathogen
- Outcome
- tested positive for BWYVe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e3“Thirteen of these samples also tested positive for BWYV”
Claim · model-written summary
Symptom expression in pea did not differ between plants co-infected with PEMV and BWYV and plants infected with PEMV alone.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- infected with both virusese4
- Interactor
- PEMVe4pathogen
- Second interactor
- BWYVBeet western yellows viruse3pathogen
- Outcome
- symptom expressione4
- Direction
- no differencese4
- Comparator
- PEMV alonee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e3“Thirteen of these samples also tested positive for BWYV”
e4“but no differences in symptom expression were observed in plants infected with both viruses or PEMV alone”
Claim · model-written summary
The PEMV isolate from pea shares 95% nucleotide sequence identity with reference PEMV-1.
- Taxon
- peaLathyrus oleraceuse5
- Material
- pea isolatee5
- Interactor
- PEMV-1
Pea enation mosaic virus 1e5pathogen - Outcome
- nucleotide sequence identitiese5
- Value
- 95%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“revealed that PEMV-1 and our pea isolate have nucleotide sequence identities of 95%”
Claim · model-written summary
This is the first report of PEMV in Spain.
- Taxon
- PeaLathyrus oleraceust0
- Interactor
- PEMVe6pathogen
- Outcome
- first report of PEMV in Spaine6
- Setting
- Spaine6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e6“this is the first report of PEMV in Spain”
Claim · model-written summary
Incidence of symptomatic pea plants ranged from 5 to 15% in commercial fields in Catalonia, Spain.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- incidence of symptomatic plantse7
- Value
- 5 to 15%e7
- Setting
- Catalonia, Spaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e7“Incidence of symptomatic plants ranged from 5 to 15% and was distributed in both small and large patches”
e0“in several commercial fields in the southern and eastern regions of Catalonia, Spain”
Claim · model-written summary
Infected pea plants showed yellow mosaic leaf symptoms that later became translucent.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- yellow mosaic leaf symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e0“Infected plants exhibited yellow mosaic leaf symptoms that later became translucent”
Claim · model-written summary
Infection before pod formation resulted in distorted and stunted pods.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- Infection prior to pod formatione0
- Outcome
- pods that were distorted and stuntede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pea and Broad Bean”
e0“Infection prior to pod formation resulted in pods that were distorted and stunted”
The same questions in other papers
infection · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
2 reporting no difference from an alternative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.