Abstract
In the present study, the effects of epibrassinolide (EBL) on indole-3-acetic acid (IAA), abscisic acid (ABA) and polyamine (PA) tissue concentrations and antioxidant potential of 7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress were investigated. EBL treatment alone or in combination with Cu enhanced free and bound IAA titers when compared with the metal alone. Modest increases in free and bound ABA contents were observed for EBL treatment alone. However, the combination of EBL with Cu caused major increases in both forms of ABA, over Cu alone. Among the PAs analyzed, only putrescine and cadaverine concentrations were enhanced by EBL treatment alone. By contrast, a significant decline in putrescine and spermine contents was found in seedlings treated with EBL plus Cu. EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) and protein contents in comparison with metal and control treatments. A major decrease in malondialdehyde content was also recorded for EBL treatments with or without Cu. An increase in phytochelatin content was also observed in seedlings treated with EBL alone or in combination with Cu. Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings.
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Claims 12
Claim · model-written summary
EBL alone or with Cu enhanced free and bound IAA titers relative to the metal alone.
- Taxon
- Raphanus sativus L.
Raphanus sativuse0 - Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatment alone or in combination with Cue1
- Outcome
- free and bound IAA titerse1
- Direction
- enhancede1
- Comparator
- the metal alonee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e1“EBL treatment alone or in combination with Cu enhanced free and bound IAA titers when compared with the metal alone”
Claim · model-written summary
EBL alone modestly increased free and bound ABA contents.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatment alonee2
- Outcome
- free and bound ABA contentse2
- Direction
- increasese2
- Qualifier
- Modeste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e2“Modest increases in free and bound ABA contents were observed for EBL treatment alone”
Claim · model-written summary
The combination of EBL with Cu caused major increases in both forms of ABA over Cu alone.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- the combination of EBL with Cue3
- Outcome
- both forms of ABAe3
- Direction
- increasese3
- Comparator
- Cu alonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e3“the combination of EBL with Cu caused major increases in both forms of ABA, over Cu alone”
Claim · model-written summary
EBL alone enhanced putrescine and cadaverine concentrations, the only polyamines enhanced.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatment alonee4
- Outcome
- putrescine and cadaverine concentrationse4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e4“only putrescine and cadaverine concentrations were enhanced by EBL treatment alone”
Claim · model-written summary
EBL plus Cu caused a significant decline in putrescine and spermine contents.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL plus Cue5
- Outcome
- putrescine and spermine contentse5
- Direction
- declinee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e5“a significant decline in putrescine and spermine contents was found in seedlings treated with EBL plus Cu”
Claim · model-written summary
EBL alone or with Cu enhanced guaiacol peroxidase, catalase and superoxide dismutase activities relative to metal and control treatments.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatments alone or in combination with Cue6
- Outcome
- guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1)e6
- Direction
- enhancede6
- Comparator
- metal and control treatmentse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e6“EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) and protein contents in comparison with metal and control treatments”
Claim · model-written summary
EBL alone or with Cu enhanced glutathione reductase activity and protein contents relative to metal and control treatments.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatments alone or in combination with Cue6
- Outcome
- glutathione reductase (EC 1.6.4.2) and protein contentse6
- Direction
- enhancede6
- Comparator
- metal and control treatmentse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e6“EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) and protein contents in comparison with metal and control treatments”
Claim · model-written summary
EBL with or without Cu caused a major decrease in malondialdehyde content.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL treatments with or without Cue7
- Outcome
- malondialdehyde contente7
- Direction
- decreasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e7“A major decrease in malondialdehyde content was also recorded for EBL treatments with or without Cu”
Claim · model-written summary
EBL alone or with Cu increased phytochelatin content in seedlings.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL alone or in combination with Cue1
- Outcome
- phytochelatin contente1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e1“An increase in phytochelatin content was also observed in seedlings treated with EBL alone or in combination with Cu”
Claim · model-written summary
EBL application improved radical scavenging activities in Cu-stressed radish seedlings.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL applicatione1
- Outcome
- radical scavenging activitiese1
- Direction
- improvemente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e1“Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings”
Claim · model-written summary
EBL application elevated deoxyribose and reducing powers in Cu-stressed radish seedlings.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL applicatione1
- Outcome
- deoxyribose and reducing powerse1
- Direction
- elevatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e1“Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings”
Claim · model-written summary
EBL application increased contents of ascorbic acid, total phenols and proline in Cu-stressed radish seedlings.
- Taxon
- Raphanus sativus L.Raphanus sativuse0
- Material
- Pusa chetkie0
- Organ / stage
- seedlingse0
- Treatment
- EBL applicatione1
- Outcome
- contents of ascorbic acid, total phenols and prolinee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress”
e1“Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings”
The same questions in other papers
copper atom · physiology: antioxidant enzymes · Raphanus sativus (radish)Positive and negative results
2 in the opposite direction
- negative
- no effect
copper atom · physiology: oxidative · Raphanus sativus (radish)Results agree
1 paper in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
24-epi-brassinolide · molecular: metabolites · Raphanus sativus (radish)Results agree
1 paper in the same direction as this paper
- positive
24-epi-brassinolide · physiology: osmolytes · Raphanus sativus (radish)Results agree
1 paper in the same direction as this paper
- positive
24-epi-brassinolide · physiology: antioxidant enzymes · Raphanus sativus (radish)Results agree
2 papers in the same direction as this paper
- positive
- positive
24-epi-brassinolide · physiology: oxidative · Raphanus sativus (radish)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.