Abstract
We partially purified three Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPa to c) which appeared in the cells undergoing a dedifferentiation process from immature pollen grains to embryogenic cells, caused by glutamine-deficiency in vitro. All the NtEPs had a highly conserved N-terminal amino acid sequence. Using degenerate oligonucleotide probes designed from the amino acid sequences, the cDNA for NtEPc was isolated from a cDNA library of pollen cultured in glutamine-free medium The cDNA sequence showed moderate homology with several type-1 copper-binding glycoproteins and with a kind of early nodulin though its function could not be predicted. Expression analysis revealed that the level of mRNA for NtEPc was high during the dedifferentiation and also in the very early period of pollen embryogenesis but it was low in the developmental process of microspores/pollen in anthers, in the in vitro maturation process and both in the stational and logarithmic growth phases of tobacco BY-2 cells. Furthermore, an acidic medium pH, which promoted the induction of dedifferentiation increased the level of mRNA for NtEPc, whereas the presence of 6-benzylaminopurine, which inhibited it, decreased the level. These results suggest that the expression of NtEPc gene is correlated with the dedifferentiation but not with pollen development or cell division.
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Claims 9
Claim · model-written summary
Three NtEP phosphoproteins appeared during pollen dedifferentiation.
- Taxon
- Nicotiana tabacum L.
Nicotiana tabacume0 - Organ / stage
- immature pollen grainse0
- Gene
- NtEPa to ce0
- Outcome
- appeared in the cells undergoing a dedifferentiation processe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPa to c) which appeared in the cells undergoing a dedifferentiation process from immature pollen grains to embryogenic cells”
Claim · model-written summary
The NtEP proteins had conserved N-terminal sequences.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacume1
- Gene
- NtEPse0
- Outcome
- highly conserved N-terminal amino acid sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the NtEPs had a highly conserved N-terminal amino acid sequence.”
e1“three Nicotiana tabacum L. embryogenic pollen-abundant phosphoproteins (NtEPa to c) which appeared in the cells undergoing a dedifferentiation process from immature pollen grains to embryogenic cells”
Claim · model-written summary
A cDNA for NtEPc was isolated from glutamine-free cultured pollen.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- NtEPce0
- Outcome
- cDNA for NtEPc was isolatede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cDNA for NtEPc was isolated from a cDNA library of pollen cultured in glutamine-free medium”
t0“Immature Tobacco Pollen Grains”
Claim · model-written summary
NtEPc cDNA showed moderate homology to copper-binding glycoproteins and an early nodulin.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- NtEPce1
- Outcome
- moderate homologye0
- Comparator
- several type-1 copper-binding glycoproteins and with a kind of early noduline0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA sequence showed moderate homology with several type-1 copper-binding glycoproteins and with a kind of early nodulin”
t0“Immature Tobacco Pollen Grains”
e1“the cDNA for NtEPc was isolated from a cDNA library of pollen cultured in glutamine-free medium”
Claim · model-written summary
NtEPc mRNA was high during dedifferentiation and early pollen embryogenesis.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- pollen embryogenesise0
- Gene
- NtEPce0
- Outcome
- level of mRNAe0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the level of mRNA for NtEPc was high during the dedifferentiation and also in the very early period of pollen embryogenesis”
t0“Immature Tobacco Pollen Grains”
Claim · model-written summary
NtEPc mRNA was low during pollen development and in vitro maturation and BY-2 growth.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- microspores/pollen in antherse0
- Gene
- NtEPce1
- Outcome
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was low in the developmental process of microspores/pollen in anthers, in the in vitro maturation process and both in the stational and logarithmic growth phases of tobacco BY-2 cells.”
e1“the cDNA for NtEPc was isolated from a cDNA library of pollen cultured in glutamine-free medium”
Claim · model-written summary
Acidic pH increased NtEPc mRNA.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- acidic medium pHe0
- Gene
- NtEPce0
- Outcome
- level of mRNA for NtEPce0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an acidic medium pH, which promoted the induction of dedifferentiation increased the level of mRNA for NtEPc”
t0“Immature Tobacco Pollen Grains”
Claim · model-written summary
6-benzylaminopurine decreased NtEPc mRNA.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- 6-benzylaminopurinee0
- Gene
- NtEPce1
- Outcome
- levele0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of 6-benzylaminopurine, which inhibited it, decreased the level.”
t0“Immature Tobacco Pollen Grains”
e1“the cDNA for NtEPc was isolated from a cDNA library of pollen cultured in glutamine-free medium”
Claim · model-written summary
The authors infer NtEPc expression correlates with dedifferentiation, but not pollen development or cell division.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- NtEPce0
- Outcome
- dedifferentiatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of NtEPc gene is correlated with the dedifferentiation but not with pollen development or cell division.”
t0“Immature Tobacco Pollen Grains”
The same questions in other papers
ph · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- negative
in vitro · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- no effect
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.