Abstract
Before micropropagation techniques can be applied to a particular species, it is crucial to optimize the method and to determine how the micropropagated plants grow in the field. Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud, after a 2 h pulse treatment with 250 μM N6‐benzyladenine. The time to first subculture after the pulse treatment influenced the yield of adventitious shoots. On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole‐3‐butyric acid for 6 h. The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks). The size of the plantlets was of importance for their survival when potted. All plantlets with a distinct stem elongated during the first growth period while some without did not. The relative height growth rate of plantlets was similar to that of seedlings. In the field the increase of height was similar for plantlets and seedlings. Plagiotropy was higher for the plantlets than for the seedlings (35 and 10% respectively). We concluded that most plantlets of P. contorta elongate normally and have a similar gross morphology to seedlings.
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Claims 11
Claim · model-written summary
Adventitious shoots formed on embryos after a benzyladenine pulse.
- Taxon
- Pinus contorta
Pinus contortae0 - Organ / stage
- embryose0
- Treatment
- a 2 h pulse treatment with 250 μM N6‐benzyladeninee0
- Outcome
- Adventitious shoots developede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud, after a 2 h pulse treatment with 250 μM N6‐benzyladenine.”
Claim · model-written summary
Time to first subculture influenced adventitious shoot yield.
- Taxon
- Pinus contortaPinus contortat0
- Treatment
- time to first subculturee0
- Outcome
- yield of adventitious shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The time to first subculture after the pulse treatment influenced the yield of adventitious shoots.”
t0“Pinus contorta”
Claim · model-written summary
On average, 68% of adventitious shoots rooted 12 weeks after auxin treatment.
- Taxon
- Pinus contortaPinus contortat0
- Treatment
- treatment with 1.25 mM indole‐3‐butyric acid for 6 he0
- Outcome
- adventitious shoots had developed rootse0
- Value
- 68%e0
- Qualifier
- 12 weeks aftere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole‐3‐butyric acid for 6 h.”
t0“Pinus contorta”
Claim · model-written summary
Auxin stimulated early rooting within six weeks.
- Taxon
- Pinus contortaPinus contortat0
- Treatment
- auxin treatmente0
- Outcome
- early rootinge0
- Direction
- stimulatede0
- Qualifier
- within 6 weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin treatment stimulated early rooting (i.e. within 6 weeks)”
t0“Pinus contorta”
Claim · model-written summary
Auxin had no effect on rooting after six weeks.
- Taxon
- Pinus contortaPinus contortat0
- Treatment
- auxin treatmente0
- Outcome
- late rootinge0
- Direction
- no effecte0
- Qualifier
- after 6 weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks).”
t0“Pinus contorta”
Claim · model-written summary
Plantlet size mattered for survival after potting.
- Taxon
- Pinus contortaPinus contortat0
- Material
- plantletse0
- Outcome
- survival when pottede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The size of the plantlets was of importance for their survival when potted.”
t0“Pinus contorta”
Claim · model-written summary
All plantlets with a distinct stem elongated in the first growth period, while some without a distinct stem did not.
- Taxon
- Pinus contortaPinus contortat0
- Material
- plantlets with a distinct steme0
- Outcome
- elongated during the first growth periode0
- Comparator
- some withoute0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All plantlets with a distinct stem elongated during the first growth period while some without did not.”
t0“Pinus contorta”
Claim · model-written summary
Plantlets and seedlings had similar relative height growth rates.
- Taxon
- Pinus contortaPinus contortat0
- Material
- plantletse0
- Outcome
- relative height growth ratee0
- Direction
- similare0
- Comparator
- seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative height growth rate of plantlets was similar to that of seedlings.”
t0“Pinus contorta”
Claim · model-written summary
Field height increase was similar in plantlets and seedlings.
- Taxon
- Pinus contortaPinus contortat0
- Material
- plantletse0
- Outcome
- increase of heighte0
- Direction
- similare0
- Comparator
- seedlingse0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the field the increase of height was similar for plantlets and seedlings.”
t0“Pinus contorta”
Claim · model-written summary
Plagiotropy was higher in plantlets (35%) than seedlings (10%).
- Taxon
- Pinus contortaPinus contortat0
- Material
- plantletse0
- Outcome
- Plagiotropye0
- Direction
- highere0
- Value
- 35 and 10%e0
- Comparator
- seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plagiotropy was higher for the plantlets than for the seedlings (35 and 10% respectively).”
t0“Pinus contorta”
Claim · model-written summary
Most plantlets elongated normally and had similar gross morphology to seedlings.
- Taxon
- P. contortaPinus contortae0
- Material
- plantletse0
- Outcome
- elongate normally and have a similar gross morphologye0
- Comparator
- seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most plantlets of P. contorta elongate normally and have a similar gross morphology to seedlings.”
The same questions in other papers
class · growth · Pinus contorta (lodgepole pine)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.