Abstract
A new member of the tobacco (Nicotiana tabacum) AP2/ERF (ethylene response factor) transcription factor family, designated NtERF5, has been isolated by yeast one-hybrid screening. In vitro, recombinant NtERF5 protein weakly binds GCC box cis-elements, which mediate pathogen-regulated transcription of several PR (pathogenesis related) genes. NtERF5 transcription is transiently activated by wounding, by infection with the bacterial pathogen Pseudomonas syringae, as well as by inoculation with Tobacco mosaic virus (TMV). In contrast, NtERF5 transcription is not enhanced after application of salicylic acid, jasmonic acid, or ethylene. Constitutive overexpression of NtERF5 (ERF5-Oex) under control of the 35S promoter results in no visible alterations in plant growth or enhanced resistance to Pseudomonas infection. Furthermore, no constitutive expression of PR genes has been observed. In contrast, ERF5-Oex plants show enhanced resistance to TMV with reference to reduced size of local hypersensitive-response lesions and impaired systemic spread of the virus. Since, in TMV-infected ERF5-Oex plants, the viral RNA accumulates only up to 10 to 30% of the wild-type level, we suggest that NtERF5-regulated gene expression is controlling resistance to viral propagation. Previous research has demonstrated that overexpression of ERF genes enhances resistance to bacterial and fungal pathogens. Here, we provide further evidence that resistance to viral infection can be engineered by overexpression of ERF transcription factors.
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Claims 13
Claim · model-written summary
NtERF5 was isolated as a new tobacco AP2/ERF transcription factor.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- NtERF5e0
- Outcome
- has been isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A new member of the tobacco (Nicotiana tabacum) AP2/ERF (ethylene response factor) transcription factor family, designated NtERF5, has been isolated by yeast one-hybrid screening.”
Claim · model-written summary
Recombinant NtERF5 weakly binds GCC box cis-elements in vitro.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro, recombinant NtERF5 protein weakly binds GCC box cis-elements”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
Wounding transiently activated NtERF5 transcription.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtERF5 transcription is transiently activated by wounding, by infection with the bacterial pathogen Pseudomonas syringae, as well as by inoculation with Tobacco mosaic virus (TMV).”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
Pseudomonas syringae infection transiently activated NtERF5 transcription.
Pseudomonas syringaeNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtERF5 transcription is transiently activated by wounding, by infection with the bacterial pathogen Pseudomonas syringae, as well as by inoculation with Tobacco mosaic virus (TMV).”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
TMV inoculation transiently activated NtERF5 transcription.
Tobacco mosaic virusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtERF5 transcription is transiently activated by wounding, by infection with the bacterial pathogen Pseudomonas syringae, as well as by inoculation with Tobacco mosaic virus (TMV).”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
Salicylic acid, jasmonic acid, or ethylene application did not enhance NtERF5 transcription.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtERF5 transcription is not enhanced after application of salicylic acid, jasmonic acid, or ethylene.”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
NtERF5 overexpression produced no visible growth alterations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive overexpression of NtERF5 (ERF5-Oex) under control of the 35S promoter results in no visible alterations in plant growth”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
NtERF5 overexpression did not enhance resistance to Pseudomonas infection.
PseudomonasNot reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“or enhanced resistance to Pseudomonas infection.”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
ERF5-Oex plants showed no constitutive PR gene expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, no constitutive expression of PR genes has been observed.”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
ERF5-Oex plants had enhanced TMV resistance with reduced local lesions.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ERF5-Oex plants show enhanced resistance to TMV with reference to reduced size of local hypersensitive-response lesions”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
ERF5-Oex plants had impaired systemic TMV spread.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“impaired systemic spread of the virus”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
TMV RNA in infected ERF5-Oex plants accumulated to 10 to 30% of the wild-type level.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in TMV-infected ERF5-Oex plants, the viral RNA accumulates only up to 10 to 30% of the wild-type level”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
Claim · model-written summary
The authors suggest NtERF5-regulated gene expression controls resistance to viral propagation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we suggest that NtERF5-regulated gene expression is controlling resistance to viral propagation.”
t0“the Tobacco Ethylene Response Transcription Factor Family”
e1“designated NtERF5”
e2“Constitutive overexpression of NtERF5 (ERF5-Oex)”
e3“Tobacco mosaic virus (TMV)”
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