Abstract
The objective of this research was to develop an efficient protocol for shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet by examining the effects of plant growth regulators, dark incubation period, gelling agents, and silver nitrate. The highest number of shoots per explant (12.3) was regenerated from leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a combination of 1 mgL(-1) of 6-benzyladenine (BA) and 2 mgL(-1) of α-naphthaleneacetic acid (NAA) under light conditions without any initial dark period. Gelrite was the most effective gelling agent for shoot regeneration among those tested, whereas the presence of silver nitrate distinctly inhibited shoot regeneration. Superior plant growth and rooting was observed on a hormone-free MS medium solidified with Gelrite. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The established protocol was applicable to shoot regeneration for four out of six cultivars tested. This research will facilitate the genetic transformation and micropropagation of Chrysanthemum cultivars.
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Claims 6
Claim · model-written summary
The highest number of shoots per explant (12.3) was regenerated from leaf explants on MS medium with 1 mg/L BA and 2 mg/L NAA under light without a dark period.
- Taxon
- Chrysanthemum
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Chrysanthemume0 - Material
- Vivid Scarlete0
- Organ / stage
- leaf explantse1
- Treatment
- combination of 1 mgL(-1) of 6-benzyladenine (BA) and 2 mgL(-1) of α-naphthaleneacetic acid (NAA)e1
- Outcome
- number of shoots per explante1
- Direction
- higheste1
- Value
- 12.3e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e1“The highest number of shoots per explant (12.3) was regenerated from leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a combination of 1 mgL(-1) of 6-benzyladenine (BA) and 2 mgL(-1) of α-naphthaleneacetic acid (NAA) under light conditions without any initial dark period”
Claim · model-written summary
Gelrite was the most effective gelling agent for shoot regeneration among those tested.
- Taxon
- ChrysanthemumChrysanthemume0
- Treatment
- Gelritee2
- Outcome
- shoot regeneratione2
- Direction
- most effectivee2
- Comparator
- among those testede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e2“Gelrite was the most effective gelling agent for shoot regeneration among those tested”
Claim · model-written summary
Silver nitrate inhibited shoot regeneration.
- Taxon
- ChrysanthemumChrysanthemume0
- Treatment
- silver nitratee3
- Outcome
- shoot regeneratione3
- Direction
- inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e3“the presence of silver nitrate distinctly inhibited shoot regeneration”
Claim · model-written summary
Superior plant growth and rooting was observed on hormone-free MS medium solidified with Gelrite.
- Taxon
- ChrysanthemumChrysanthemume0
- Treatment
- hormone-free MS medium solidified with Gelritee4
- Outcome
- plant growth and rootinge4
- Direction
- Superiore4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e4“Superior plant growth and rooting was observed on a hormone-free MS medium solidified with Gelrite”
Claim · model-written summary
Regenerated plants showed no ploidy variation compared with the mother plant grown under greenhouse conditions.
- Taxon
- ChrysanthemumChrysanthemume0
- Outcome
- ploidy variatione5
- Comparator
- the mother plante5
- Setting
- greenhouse conditionse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e5“Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions”
Claim · model-written summary
The established protocol was applicable to shoot regeneration for four out of six cultivars tested.
- Taxon
- ChrysanthemumChrysanthemume0
- Outcome
- shoot regeneratione6
- Value
- four out of six cultivarse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet”
e6“The established protocol was applicable to shoot regeneration for four out of six cultivars tested”
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- no effect
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See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.