Abstract
BEL1- and KNOTTED1-type proteins are transcription factors from the three-amino-loop-extension superclass that interact in a tandem complex to regulate the expression of target genes. In potato (Solanum tuberosum), StBEL5 and its Knox protein partner regulate tuberization by targeting genes that control growth. RNA movement assays demonstrated that StBEL5 transcripts move through the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement to stolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts. Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark, promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor, binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motif in the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolon tips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results in the reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5/POTH1 binding specificity to the motifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggest that, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.
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Claims 8
Claim · model-written summary
StBEL5 RNA movement to roots correlated with increased root growth and morphology changes.
- Taxon
- potato (Solanum tuberosum)
Solanum tuberosume1 - Organ / stage
- rootse0
- Gene
- StBEL5e0
- Outcome
- increased growth, changes in morphologye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StBEL5 RNA movement to roots correlated with accumulation of GA2-oxidase1, YUCCA1a and ISOPENTENYL TRANSFERASE transcripts.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Organ / stage
- rootse0
- Gene
- StBEL5e0
- Outcome
- accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcriptse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
Light induced StBEL5 transcription in leaves, independent of photoperiod.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Organ / stage
- leavese0
- Treatment
- lighte0
- Gene
- StBEL5e0
- Outcome
- Transcription of StBEL5e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
Short days enhanced StBEL5 promoter activity in dark grown stolon tips.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Organ / stage
- stolon tipse0
- Treatment
- short dayse0
- Gene
- StBEL5e2
- Outcome
- promoter activitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in stolon tips growing in the dark, promoter activity is enhanced by short days”
e1“potato (Solanum tuberosum)”
e2“Transcription of StBEL5 in leaves is induced by light”
Claim · model-written summary
The StBEL5 and POTH1 heterodimer binds a tandem TTGAC-TTGAC motif needed for transcriptional regulation.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Gene
- StBEL5 and POTH1e0
- Outcome
- binds to a tandem TTGAC-TTGAC motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor, binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription.”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
An inverted tandem motif with TTGAC motifs on opposite strands was found in the StBEL5 promoter.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Gene
- StBEL5e0
- Outcome
- inverted tandem motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The discovery of an inverted tandem motif in the StBEL5 promoter with TTGAC motifs on opposite strands”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
Deleting one TTGAC motif from the StBEL5 promoter reduced GUS activity in new tubers and roots.
- Taxon
- potatoSolanum tuberosume1
- Organ / stage
- new tubers and rootse0
- Treatment
- deletion of one of the TTGAC motifse0
- Gene
- StBEL5e0
- Outcome
- GUS activitye0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of one of the TTGAC motifs from the StBEL5 promoter results in the reduction of GUS activity in new tubers and roots”
e1“Using transgenic potato lines”
Claim · model-written summary
Gel-shift assays showed BEL5/POTH1 binding specificity to StBEL5 and StGA2-oxidase1 promoter motifs.
- Taxon
- potato (Solanum tuberosum)Solanum tuberosume1
- Gene
- BEL5/POTH1e0
- Outcome
- BEL5/POTH1 binding specificitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gel-shift assays demonstrate BEL5/POTH1 binding specificity to the motifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter.”
e1“potato (Solanum tuberosum)”
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