Abstract
The objective of the current study was to establish an efficient and reproducible in vitro plant regeneration protocol using cotyledonary explant for Citrullus lanatus cv. Round Dragon. To achieve optimal conditions for adventitious shoot induction, cotyledon explants of 5-day-old seedlings, 7-day-old seedlings and 9-day-old seedlings were tested for regeneration potential on Murashige and Skoog (MS) media supplemented with 2.3 mg L-1 BAP. Results showed that high frequency of in vitro adventitious shoot regeneration was induced from the proximal region of 5-day-old seedlings (93%) with 19.80±0.99 shoots per responding explant after 6 weeks. Adventitious shoots induced from 5-day-old seedlings after 6 weeks were transferred to MS shoot regeneration medium without plant growth regulator for shoot elongation for 4 weeks. The influence of various concentrations of IBA, IAA and NAA on root initiation was examined on half-strength and full-strength of MS rooting medium. The best response for root initiation was obtained from the microshoots grown in full-strength MS rooting medium compared to the half-strength MS rooting medium. Furthermore, IBA was more efficient in promoting root induction than IAA and NAA, resulting in a higher rate of root initiation (100%) at the concentration of 0.1 mg L-1 IBA. Therefore, elongated shoots were rooted in MS medium supplemented with 0.1 mg L-1 IBA for 3 weeks. Rooted plantlets were acclimatized successfully under ex vitro conditions.
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Claims 6
Claim · model-written summary
Proximal cotyledons from five-day seedlings achieved 93% adventitious shoot regeneration after six weeks.
- Taxon
- Citrullus lanatus cv. Round Dragon
Citrullus lanatust0 - Organ / stage
- proximal region of 5-day-old seedlingse0
- Outcome
- in vitro adventitious shoot regeneratione0
- Value
- 93%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high frequency of in vitro adventitious shoot regeneration was induced from the proximal region of 5-day-old seedlings (93%) with 19.80±0.99 shoots per responding explant after 6 weeks.”
t0“Citrullus lanatus cv. Round Dragon”
Claim · model-written summary
Responding cotyledon explants produced 19.80±0.99 shoots after six weeks.
- Taxon
- Citrullus lanatus cv. Round DragonCitrullus lanatust0
- Outcome
- shoots per responding explante0
- Value
- 19.80±0.99 shoots per responding explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with 19.80±0.99 shoots per responding explant after 6 weeks.”
t0“Citrullus lanatus cv. Round Dragon”
Claim · model-written summary
Full-strength MS rooting medium gave a better root initiation response than half-strength medium.
- Taxon
- Citrullus lanatus cv. Round DragonCitrullus lanatust0
- Treatment
- full-strength MS rooting mediume0
- Outcome
- response for root initiatione0
- Direction
- beste0
- Comparator
- half-strength MS rooting mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best response for root initiation was obtained from the microshoots grown in full-strength MS rooting medium compared to the half-strength MS rooting medium.”
t0“Citrullus lanatus cv. Round Dragon”
Claim · model-written summary
IBA promoted root induction more efficiently than IAA and NAA.
- Taxon
- Citrullus lanatus cv. Round DragonCitrullus lanatust0
- Treatment
- IBAe0
- Outcome
- root inductione0
- Direction
- more efficiente0
- Comparator
- IAA and NAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IBA was more efficient in promoting root induction than IAA and NAA”
t0“Citrullus lanatus cv. Round Dragon”
Claim · model-written summary
At 0.1 mg L-1 IBA, root initiation reached 100%.
- Taxon
- Citrullus lanatus cv. Round DragonCitrullus lanatust0
- Treatment
- 0.1 mg L-1 IBAe0
- Outcome
- rate of root initiatione0
- Direction
- highere0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in a higher rate of root initiation (100%) at the concentration of 0.1 mg L-1 IBA.”
t0“Citrullus lanatus cv. Round Dragon”
Claim · model-written summary
Rooted plantlets were successfully acclimatized outside in vitro conditions.
- Taxon
- Citrullus lanatus cv. Round DragonCitrullus lanatust0
- Organ / stage
- Rooted plantletse0
- Outcome
- acclimatized successfully under ex vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted plantlets were acclimatized successfully under ex vitro conditions.”
t0“Citrullus lanatus cv. Round Dragon”
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