Abstract
The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene is a central embryonic regulator that serves critical roles both early and late during embryo development. LEC2 is required for the maintenance of suspensor morphology, specification of cotyledon identity, progression through the maturation phase, and suppression of premature germination. We cloned the LEC2 gene on the basis of its chromosomal position and showed that the predicted polypeptide contains a B3 domain, a DNA-binding motif unique to plants that is characteristic of several transcription factors. We showed that LEC2 RNA accumulates primarily during seed development, consistent with our finding that LEC2 shares greatest similarity with the B3 domain transcription factors that act primarily in developing seeds, VIVIPAROUS1/ABA INSENSITIVE3 and FUSCA3. Ectopic, postembryonic expression of LEC2 in transgenic plants induces the formation of somatic embryos and other organ-like structures and often confers embryonic characteristics to seedlings. Together, these results suggest that LEC2 is a transcriptional regulator that establishes a cellular environment sufficient to initiate embryo development.
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Claims 10
Claim · model-written summary
LEC2 is required to maintain suspensor morphology.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- LEC2e0
- Outcome
- maintenance of suspensor morphologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 is required for the maintenance of suspensor morphology”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
LEC2 is required for cotyledon identity.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- specification of cotyledon identitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 is required for the maintenance of suspensor morphology, specification of cotyledon identity”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
LEC2 is required for progression through embryo maturation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- progression through the maturation phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 is required for the maintenance of suspensor morphology, specification of cotyledon identity, progression through the maturation phase”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
LEC2 suppresses premature germination.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- suppression of premature germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 is required for the maintenance of suspensor morphology, specification of cotyledon identity, progression through the maturation phase, and suppression of premature germination”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
Cloned LEC2 encodes a predicted polypeptide containing a B3 domain.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- predicted polypeptide contains a B3 domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We cloned the LEC2 gene on the basis of its chromosomal position and showed that the predicted polypeptide contains a B3 domain”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
LEC2 RNA accumulates primarily during seed development.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- seed developmente0
- Gene
- LEC2e0
- Outcome
- LEC2 RNA accumulatese0
- Direction
- primarilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 RNA accumulates primarily during seed development”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
LEC2 is most similar to VIVIPAROUS1/ABA INSENSITIVE3 and FUSCA3.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- greatest similaritye0
- Comparator
- VIVIPAROUS1/ABA INSENSITIVE3 and FUSCA3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 shares greatest similarity with the B3 domain transcription factors that act primarily in developing seeds, VIVIPAROUS1/ABA INSENSITIVE3 and FUSCA3”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
Ectopic postembryonic LEC2 expression induces somatic embryos and other organ-like structures.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Ectopic, postembryonic expression of LEC2e0
- Gene
- LEC2e0
- Outcome
- somatic embryos and other organ-like structurese0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic, postembryonic expression of LEC2 in transgenic plants induces the formation of somatic embryos and other organ-like structures”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
Ectopic postembryonic LEC2 expression often gives seedlings embryonic characteristics.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Ectopic, postembryonic expression of LEC2e0
- Gene
- LEC2e0
- Outcome
- embryonic characteristics to seedlingse0
- Direction
- conferse0
- Qualifier
- oftene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic, postembryonic expression of LEC2 in transgenic plants induces the formation of somatic embryos and other organ-like structures and often confers embryonic characteristics to seedlings”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
Claim · model-written summary
The authors infer that LEC2 establishes a cellular environment sufficient to initiate embryo development.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- LEC2e0
- Outcome
- initiate embryo developmente0
- Qualifier
- cellular environmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LEC2 is a transcriptional regulator that establishes a cellular environment sufficient to initiate embryo development”
e1“The Arabidopsis LEAFY COTYLEDON2 (LEC2) gene”
The same questions in other papers
transgenic · growth · ArabidopsisPositive and negative results
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- negative
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See all 85 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.