Paper · 1997 · The Plant Journal

The maize gene empty pericarp‐2 is required for progression beyond early stages of embryogenesis

Michael J. Scanlon; Alan M. Myers; Richard G. Schneeberger; et al.

7
claims
13
verbatim quotes
18
citations
2
research areas
—
Not open access

Abstract

Summary The defective kernel mutation empty pericarp2‐R ( emp2‐R ) causes retardation and subsequent abortion of maize kernel development. Analyses of genetic aneuploid kernels indicate that the embryo phenotype is not dependent on the endosperm genotype; the mutation conditions embryo defects even in the presence of a normal endosperm. Embryos reach an abnormal coleoptilar stage before aborting and disintegrating. The mutants form primary embryonic organs only; the scutellum and coleoptile develop, but no leaves are formed. Immunohisto‐localization studies utilized KNOX homeodomain proteins as markers of meristem formation and identity. These analyses indicate that the shoot meristem forms in emp2‐R mutant embryos, but does not mature to a tunica‐corpus shape. No evidence of leaf founder cell initialization was revealed in the mutant meristems. These data indicate that the emp2 gene may be required for embryogenic patterning beyond the coleoptilar stage of development.

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Claims 7

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

emp2-R mutation retarded and aborted kernel development.

Taxon
maizeZea mayse0
Gene
empty pericarp2‐Re0
Outcome
retardation and subsequent abortion of maize kernel developmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The defective kernel mutation empty pericarp2‐R ( emp2‐R ) causes retardation and subsequent abortion of maize kernel development.”

✓ located verbatim in the source · abstract, characters 8–138 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Embryo defects persisted despite normal endosperm.

Taxon
maizeZea mayst0
Outcome
embryo defectse0
Comparator
normal endosperme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the mutation conditions embryo defects even in the presence of a normal endosperm.”

✓ located verbatim in the source · abstract, characters 256–338 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Mutant embryos reached an abnormal coleoptilar stage before aborting.

Taxon
maizeZea mayst0
Organ / stage
Embryose0
Outcome
abnormal coleoptilar stage before aborting and disintegratinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Embryos reach an abnormal coleoptilar stage before aborting and disintegrating.”

✓ located verbatim in the source · abstract, characters 339–418 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Mutants formed scutellum and coleoptile but no leaves.

Taxon
maizeZea mayst0
Outcome
scutellum and coleoptile develop, but no leaves are formede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The mutants form primary embryonic organs only; the scutellum and coleoptile develop, but no leaves are formed.”

✓ located verbatim in the source · abstract, characters 419–530 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Shoot meristem formed but failed to mature to a tunica-corpus shape.

Taxon
maizeZea mayst0
Gene
emp2‐Re0
Outcome
shoot meristem forms in emp2‐R mutant embryos, but does not mature to a tunica‐corpus shapee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the shoot meristem forms in emp2‐R mutant embryos, but does not mature to a tunica‐corpus shape.”

✓ located verbatim in the source · abstract, characters 675–771 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

No leaf founder cell initiation was seen in mutant meristems.

Taxon
maizeZea mayst0
Outcome
leaf founder cell initializatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No evidence of leaf founder cell initialization was revealed in the mutant meristems.”

✓ located verbatim in the source · abstract, characters 772–857 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Authors infer emp2 is required for patterning beyond the coleoptilar stage.

Taxon
maizeZea mayst0
Gene
emp2 genee0
Outcome
embryogenic patterning beyond the coleoptilar stage of developmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the emp2 gene may be required for embryogenic patterning beyond the coleoptilar stage of development.”

✓ located verbatim in the source · abstract, characters 883–984 · show in text ↑
t0

“maize gene”

✓ located verbatim in the source · title, characters 4–14 · show in text ↑