Abstract
In order to study chloroplast biogenesis, we chose natural variegated Epipremnum aureum (golden pothos) and regenerated pale yellow, variegated and green plants from all three types of tissue explants. The percentage of three types of regenerated shoots from three different explants was very close. Regenerated plants have been maintained for a year and show no sign of a colour switch. By comparing their protein profiles, two major differences between pale yellow and green plants were observed at the 15 and 40 to 50 kDa proteins. Moreover, pale yellow plants had unexpected high molecular mass proteins (greater than 60 kDa). Both variegated and green plants had more chlorophyll (Chl) a than Chl b, the ratios were about 1.46 and 1.93, respectively. In contrast, the pale yellow plants not only had less total Chl, but also the reduction of Chl a was much greater than Chl b, resulting in a higher content of Chl b than Chl a. Microscopic analysis revealed that pale yellow plants contained predominantly undeveloped chloroplasts with low Chl contents, even though their mesophyll cells were similar to green and variegated plants. PCR amplification of chloroplast DNA with 14 universal chloroplast primers did not reveal any difference among these regenerated plants.
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Claims 11
Claim · model-written summary
The proportions of three regenerated shoot types were close across three explant types.
- Taxon
- Epipremnum aureum
Epipremnum aureumt0 - Outcome
- percentage of three types of regenerated shootse0
- Direction
- very closee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of three types of regenerated shoots from three different explants was very close.”
t0“Epipremnum aureum”
Claim · model-written summary
Regenerated plants showed no colour switch after one year.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Outcome
- no sign of a colour switche0
- Qualifier
- for a yeare0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants have been maintained for a year and show no sign of a colour switch.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow and green plants differed in their 15 and 40 to 50 kDa protein profiles.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- pale yellowe0
- Outcome
- proteinse0
- Value
- 15 and 40 to 50 kDae0
- Comparator
- green plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two major differences between pale yellow and green plants were observed at the 15 and 40 to 50 kDa proteins.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow plants had unexpected proteins above 60 kDa.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- pale yellow plantse0
- Outcome
- high molecular mass proteinse0
- Value
- greater than 60 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pale yellow plants had unexpected high molecular mass proteins (greater than 60 kDa).”
t0“Epipremnum aureum”
Claim · model-written summary
Variegated plants had more chlorophyll a than b, with a ratio about 1.46.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- variegatede0
- Outcome
- chlorophyll (Chl) a than Chl be0
- Direction
- moree0
- Value
- 1.46e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both variegated and green plants had more chlorophyll (Chl) a than Chl b, the ratios were about 1.46 and 1.93, respectively.”
t0“Epipremnum aureum”
Claim · model-written summary
Green plants had more chlorophyll a than b, with a ratio about 1.93.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- green plantse0
- Outcome
- chlorophyll (Chl) a than Chl be0
- Direction
- moree0
- Value
- 1.93e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both variegated and green plants had more chlorophyll (Chl) a than Chl b, the ratios were about 1.46 and 1.93, respectively.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow plants had less total chlorophyll than the other regenerated plants.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- pale yellow plantse0
- Outcome
- total Chle0
- Direction
- lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pale yellow plants not only had less total Chl, but also the reduction of Chl a was much greater than Chl b, resulting in a higher content of Chl b than Chl a.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow plants lost more chlorophyll a than b and consequently had more b than a.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- pale yellow plantse0
- Outcome
- content of Chl b than Chl ae0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pale yellow plants not only had less total Chl, but also the reduction of Chl a was much greater than Chl b, resulting in a higher content of Chl b than Chl a.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow plants had predominantly undeveloped, low-chlorophyll chloroplasts.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Material
- pale yellow plantse0
- Organ / stage
- mesophyll cellse0
- Outcome
- undeveloped chloroplasts with low Chl contentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pale yellow plants contained predominantly undeveloped chloroplasts with low Chl contents, even though their mesophyll cells were similar to green and variegated plants.”
t0“Epipremnum aureum”
Claim · model-written summary
Pale yellow mesophyll cells resembled those of green and variegated plants.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Outcome
- mesophyll cells were similare0
- Comparator
- green and variegated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their mesophyll cells were similar to green and variegated plants.”
t0“Epipremnum aureum”
Claim · model-written summary
PCR detected no chloroplast DNA difference among regenerated plant types.
- Taxon
- Epipremnum aureumEpipremnum aureumt0
- Outcome
- chloroplast DNAe0
- Direction
- did not reveal any differencee0
- Value
- 14 universal chloroplast primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR amplification of chloroplast DNA with 14 universal chloroplast primers did not reveal any difference among these regenerated plants.”
t0“Epipremnum aureum”