Abstract
ABSTRACT Methods for quantitative estimation of nitrate and nitrite were compared. Levels of these ions were measured in vegetables and grain products and effects of processing on nitrate and nitrite levels in carrots were measured. These data allow more accurate estimation of ingestion levels and suggest means to reduce exposure to these ions. High performance liquid chromatography (HPLC) had better precision and recoveries than either a classical Cd‐Griess method nitrate or a Griess method for nitrite. Nitrate concentration by HPLC varied greatly within and between vegetables, ranging from 1 μmol/100g in mushrooms to 5000 μmol/100g in celery and averaging 9.7 ± 4.4 μmol/100g in grains. Nitrate levels in vegetables sold as “organic” were not different (p<0.05) from conventional vegetables. No nitrite was detected in either vegetables or grains. Nitrate was unevenly distributed in carrots with the core having the most. Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels and no nitrite developed. Fifty‐seven percent of nitrate was leached into cooking liquid when frozen carrots were boiled. Thirty‐two percent of nitrate was lost during canning and 47% of the remainder was in the liquid. No nitrite developed during 10 wk of canned storage.
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Claims 12
Claim · model-written summary
HPLC had better precision and recovery for nitrate and nitrite than the comparison methods.
- Taxon
- Carrots
Daucus carotat0 - Outcome
- precision and recoveriese0
- Direction
- bettere0
- Comparator
- a classical Cd‐Griess method nitrate or a Griess method for nitritee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“High performance liquid chromatography (HPLC) had better precision and recoveries than either a classical Cd‐Griess method nitrate or a Griess method for nitrite”
t0“Raw Carrots, Processed Carrots”
Claim · model-written summary
Nitrate concentration varied greatly among vegetables, from mushrooms to celery.
Verbatim
e0“Nitrate concentration by HPLC varied greatly within and between vegetables, ranging from 1 μmol/100g in mushrooms to 5000 μmol/100g in celery”
Claim · model-written summary
Grains averaged 9.7 ± 4.4 μmol/100g nitrate.
Verbatim
e0“averaging 9.7 ± 4.4 μmol/100g in grains”
Claim · model-written summary
Organic vegetables did not differ in nitrate from conventional vegetables.
Verbatim
e0“Nitrate levels in vegetables sold as “organic” were not different (p<0.05) from conventional vegetables”
Claim · model-written summary
No nitrite was detected in vegetables or grains.
Verbatim
e0“No nitrite was detected in either vegetables or grains”
Claim · model-written summary
Carrot core contained the most nitrate.
- Taxon
- carrots
Daucus carotae0 - Organ / stage
- coree0
- Outcome
- Nitratee0
- Direction
- moste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Nitrate was unevenly distributed in carrots with the core having the most”
Claim · model-written summary
Frozen storage for 10 weeks did not alter carrot nitrate levels.
- Taxon
- carrots
Daucus carotae0 - Treatment
- Storage of carrots at ‐18°C for 10 wke0
- Outcome
- nitrate levelse0
- Direction
- did not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels”
Claim · model-written summary
Nitrite did not develop during 10 weeks of frozen carrot storage.
- Taxon
- carrots
Daucus carotae0 - Treatment
- Storage of carrots at ‐18°C for 10 wke0
- Outcome
- no nitrite developede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels and no nitrite developed”
Claim · model-written summary
Boiling frozen carrots leached 57% of nitrate into cooking liquid.
- Taxon
- carrots
Daucus carotae0 - Treatment
- boilede0
- Outcome
- nitrate was leached into cooking liquide0
- Direction
- leachede0
- Value
- Fifty‐seven percente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Fifty‐seven percent of nitrate was leached into cooking liquid when frozen carrots were boiled”
Claim · model-written summary
Canning removed 32% of carrot nitrate.
- Taxon
- Carrots
Daucus carotat0 - Treatment
- canninge0
- Outcome
- nitratee0
- Direction
- loste0
- Value
- Thirty‐two percente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Thirty‐two percent of nitrate was lost during canning”
t0“Raw Carrots, Processed Carrots”
Claim · model-written summary
After canning, 47% of remaining nitrate was in the liquid.
- Taxon
- Carrots
Daucus carotat0 - Outcome
- the remainder was in the liquide0
- Value
- 47%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“47% of the remainder was in the liquid”
t0“Raw Carrots, Processed Carrots”
Claim · model-written summary
Nitrite did not develop during 10 weeks of canned storage.
- Taxon
- Carrots
Daucus carotat0 - Outcome
- No nitrite developede0
- Qualifier
- 10 wk of canned storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“No nitrite developed during 10 wk of canned storage”
t0“Raw Carrots, Processed Carrots”
The same questions in other papers
storage · molecular: metabolites · Daucus carota (carrot)Positive and negative results
6 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →storage · nutrient: content · Daucus carota (carrot)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.