Paper · 1987 · Journal of Food Science

Nitrate and Nitrite Methods of Analysis and Levels in Raw Carrots, Processed Carrots and in Selected Vegetables and Grain Products

BARBARA E. SCHUSTER; KEN LEE

12
claims
16
verbatim quotes
53
citations
2
research areas
—
Not open access

Abstract

ABSTRACT Methods for quantitative estimation of nitrate and nitrite were compared. Levels of these ions were measured in vegetables and grain products and effects of processing on nitrate and nitrite levels in carrots were measured. These data allow more accurate estimation of ingestion levels and suggest means to reduce exposure to these ions. High performance liquid chromatography (HPLC) had better precision and recoveries than either a classical Cd‐Griess method nitrate or a Griess method for nitrite. Nitrate concentration by HPLC varied greatly within and between vegetables, ranging from 1 μmol/100g in mushrooms to 5000 μmol/100g in celery and averaging 9.7 ± 4.4 μmol/100g in grains. Nitrate levels in vegetables sold as “organic” were not different (p<0.05) from conventional vegetables. No nitrite was detected in either vegetables or grains. Nitrate was unevenly distributed in carrots with the core having the most. Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels and no nitrite developed. Fifty‐seven percent of nitrate was leached into cooking liquid when frozen carrots were boiled. Thirty‐two percent of nitrate was lost during canning and 47% of the remainder was in the liquid. No nitrite developed during 10 wk of canned storage.

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Claims 12

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

HPLC had better precision and recovery for nitrate and nitrite than the comparison methods.

Taxon
CarrotsDaucus carotat0
Outcome
precision and recoveriese0
Direction
bettere0
Comparator
a classical Cd‐Griess method nitrate or a Griess method for nitritee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“High performance liquid chromatography (HPLC) had better precision and recoveries than either a classical Cd‐Griess method nitrate or a Griess method for nitrite”

✓ located verbatim in the source · abstract, characters 347–508 · show in text ↑
t0

“Raw Carrots, Processed Carrots”

✓ located verbatim in the source · title, characters 54–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Nitrate concentration varied greatly among vegetables, from mushrooms to celery.

Taxon
vegetablese0
Outcome
Nitrate concentratione0
Direction
varied greatlye0
Value
1 μmol/100ge0
Qualifier
5000 μmol/100ge0

Verbatim

e0

“Nitrate concentration by HPLC varied greatly within and between vegetables, ranging from 1 μmol/100g in mushrooms to 5000 μmol/100g in celery”

✓ located verbatim in the source · abstract, characters 510–651 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Grains averaged 9.7 ± 4.4 μmol/100g nitrate.

Taxon
grainse0
Outcome
averaginge0
Value
9.7 ± 4.4 μmol/100ge0

Verbatim

e0

“averaging 9.7 ± 4.4 μmol/100g in grains”

✓ located verbatim in the source · abstract, characters 656–695 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Organic vegetables did not differ in nitrate from conventional vegetables.

Taxon
vegetablese0
Material
vegetables sold as “organic”e0
Outcome
Nitrate levelse0
Direction
not differente0
Comparator
conventional vegetablese0

Verbatim

e0

“Nitrate levels in vegetables sold as “organic” were not different (p<0.05) from conventional vegetables”

✓ located verbatim in the source · abstract, characters 697–800 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No nitrite was detected in vegetables or grains.

Taxon
vegetables or grainse0
Outcome
No nitrite was detectede0

Verbatim

e0

“No nitrite was detected in either vegetables or grains”

✓ located verbatim in the source · abstract, characters 802–856 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Carrot core contained the most nitrate.

Taxon
carrotsDaucus carotae0
Organ / stage
coree0
Outcome
Nitratee0
Direction
moste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Nitrate was unevenly distributed in carrots with the core having the most”

✓ located verbatim in the source · abstract, characters 858–931 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Frozen storage for 10 weeks did not alter carrot nitrate levels.

Taxon
carrotsDaucus carotae0
Treatment
Storage of carrots at ‐18°C for 10 wke0
Outcome
nitrate levelse0
Direction
did not altere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels”

✓ located verbatim in the source · abstract, characters 933–999 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nitrite did not develop during 10 weeks of frozen carrot storage.

Taxon
carrotsDaucus carotae0
Treatment
Storage of carrots at ‐18°C for 10 wke0
Outcome
no nitrite developede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Storage of carrots at ‐18°C for 10 wk did not alter nitrate levels and no nitrite developed”

✓ located verbatim in the source · abstract, characters 933–1024 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Boiling frozen carrots leached 57% of nitrate into cooking liquid.

Taxon
carrotsDaucus carotae0
Treatment
boilede0
Outcome
nitrate was leached into cooking liquide0
Direction
leachede0
Value
Fifty‐seven percente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Fifty‐seven percent of nitrate was leached into cooking liquid when frozen carrots were boiled”

✓ located verbatim in the source · abstract, characters 1026–1120 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Canning removed 32% of carrot nitrate.

Taxon
CarrotsDaucus carotat0
Treatment
canninge0
Outcome
nitratee0
Direction
loste0
Value
Thirty‐two percente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Thirty‐two percent of nitrate was lost during canning”

✓ located verbatim in the source · abstract, characters 1122–1175 · show in text ↑
t0

“Raw Carrots, Processed Carrots”

✓ located verbatim in the source · title, characters 54–84 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

After canning, 47% of remaining nitrate was in the liquid.

Taxon
CarrotsDaucus carotat0
Outcome
the remainder was in the liquide0
Value
47%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“47% of the remainder was in the liquid”

✓ located verbatim in the source · abstract, characters 1180–1218 · show in text ↑
t0

“Raw Carrots, Processed Carrots”

✓ located verbatim in the source · title, characters 54–84 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Nitrite did not develop during 10 weeks of canned storage.

Taxon
CarrotsDaucus carotat0
Outcome
No nitrite developede0
Qualifier
10 wk of canned storagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No nitrite developed during 10 wk of canned storage”

✓ located verbatim in the source · abstract, characters 1220–1271 · show in text ↑
t0

“Raw Carrots, Processed Carrots”

✓ located verbatim in the source · title, characters 54–84 · show in text ↑

storage · molecular: metabolites · Daucus carota (carrot)Positive and negative results

12 papers: 5 positive, 7 negative or no effect

6 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

storage · nutrient: content · Daucus carota (carrot)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.