Abstract
Two yeast genes, FRE1 and FRE2 (encoding Fe(III) reductases) were placed under the control of the cauliflower mosaic virus 35S promoter and introduced into tobacco (Nicotiana tabacum L.) via Agrobacterium tumefaciens-mediated transformation. Homozygous lines containing FRE1, FRE2, or FRE1 plus FRE2 were generated. Northern-blot analyses revealed mRNA of two different sizes in FRE1 lines, whereas all FRE2 lines had mRNA only of the expected length. Fe(III) reduction, chlorophyll contents, and Fe levels were determined in transgenic and control plants under Fe-sufficient and Fe-deficient conditions. In a normal growth environment, the highest root Fe(III) reduction, 4-fold higher than in controls, occurred in the double transformant (FRE1 + FRE2). Elevated Fe(III) reduction was also observed in all FRE2 and some FRE1 lines. The increased Fe(III) reduction occurred along the entire length of the roots and on shoot sections. FRE2 and double transformants were more tolerant to Fe deficiency in hydroponic culture, as shown by higher chlorophyll and Fe concentrations in younger leaves, whereas FRE1 transformants did not differ from the controls. Overall, the beneficial effects of FRE2 were consistent, suggesting that FRE2 may be used to improve Fe efficiency in crop plants.
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Claims 10
Claim · model-written summary
FRE1 lines had two mRNA sizes.
- Taxon
- tobacco
Nicotiana tabacumt0 - Gene
- FRE1e0
- Outcome
- mRNA of two different sizese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern-blot analyses revealed mRNA of two different sizes in FRE1 lines”
t0“Transgenic Tobacco”
Claim · model-written summary
All FRE2 lines had mRNA of expected length.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- FRE2e0
- Outcome
- mRNA only of the expected lengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all FRE2 lines had mRNA only of the expected length”
t0“Transgenic Tobacco”
Claim · model-written summary
The double transformant had fourfold higher root iron reduction than controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- double transformant (FRE1 + FRE2)e0
- Treatment
- FRE1 + FRE2e0
- Outcome
- root Fe(III) reductione0
- Direction
- highere0
- Value
- 4-folde0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest root Fe(III) reduction, 4-fold higher than in controls, occurred in the double transformant (FRE1 + FRE2)”
t0“Transgenic Tobacco”
Claim · model-written summary
All FRE2 lines had elevated iron reduction.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- FRE2e0
- Outcome
- Fe(III) reductione0
- Direction
- Elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated Fe(III) reduction was also observed in all FRE2 and some FRE1 lines”
t0“Transgenic Tobacco”
Claim · model-written summary
Some FRE1 lines had elevated iron reduction.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- FRE1e0
- Outcome
- Fe(III) reductione0
- Direction
- Elevatede0
- Qualifier
- somee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated Fe(III) reduction was also observed in all FRE2 and some FRE1 lines”
t0“Transgenic Tobacco”
Claim · model-written summary
Increased reduction occurred across roots and shoot sections.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- roots and on shoot sectionse0
- Outcome
- Fe(III) reductione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increased Fe(III) reduction occurred along the entire length of the roots and on shoot sections”
t0“Transgenic Tobacco”
Claim · model-written summary
FRE2 transformants had greater iron deficiency tolerance with higher young leaf chlorophyll and iron.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- FRE2e0
- Organ / stage
- younger leavese0
- Treatment
- Fe deficiencye0
- Outcome
- chlorophyll and Fe concentrationse0
- Direction
- highere0
- Comparator
- controlse0
- Setting
- hydroponic culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FRE2 and double transformants were more tolerant to Fe deficiency in hydroponic culture, as shown by higher chlorophyll and Fe concentrations in younger leaves, whereas FRE1 transformants did not differ from the controls”
t0“Transgenic Tobacco”
Claim · model-written summary
Double transformants had greater iron deficiency tolerance with higher young leaf chlorophyll and iron.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- double transformantse0
- Organ / stage
- younger leavese0
- Treatment
- Fe deficiencye0
- Outcome
- chlorophyll and Fe concentrationse0
- Direction
- highere0
- Comparator
- controlse0
- Setting
- hydroponic culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FRE2 and double transformants were more tolerant to Fe deficiency in hydroponic culture, as shown by higher chlorophyll and Fe concentrations in younger leaves, whereas FRE1 transformants did not differ from the controls”
t0“Transgenic Tobacco”
Claim · model-written summary
FRE1 transformants did not differ from controls under iron deficiency.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- FRE1 transformantse0
- Outcome
- did not differe0
- Direction
- did not differe0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FRE1 transformants did not differ from the controls”
t0“Transgenic Tobacco”
Claim · model-written summary
Authors suggest FRE2 for improving crop iron efficiency.
Verbatim
e0“FRE2 may be used to improve Fe efficiency in crop plants”
The same questions in other papers
iron atom · physiology: pigments · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.