Abstract
Random mutagenesis with the transposon Tn5tac1 in Xanthomonas axonopodis pathovar citri, the causal agent of citrus canker, generated four mutants with altered pathogenicity. These mutants were classified into three groups: (i) the nonpathogenic (NP) mutants XT10 and XT122, which did not induce any visible symptoms in the host; (ii) the WS - mutant XT27, which induced a callus-like lesion but not a watersoaked lesion; and (iii) the CL - mutant XT37, which was unable to induce a callus-like eruption but did induce the formation of a watersoaked lesion around the infection site. The NP mutants failed to grow in planta, whereas the WS - and CL - mutants showed a reduced growth rate relative to that of the wild type. Co-inoculation of leaves with the WS - and CL - mutants did not result in complementation of their respective defects. The extent of extracellular accumulation of polysaccharide, protease, and amylase activities by each of the mutants was similar to that of the wild type. The extracellular activity of polygalacturonate lyase of XT27 was reduced relative to that of the wild type and other mutants. Unlike the wild type and other mutants, XT27 also required glutamic acid for growth in culture. Southern blot hybridization revealed that each of the mutants resulted from transposon insertion at a single site; the insertion sites for XT10 and XT27 were located in the chromosome, whereas those for XT37 and XT122 were located in the indigenous plasmids. These results provide evidence that bacterial genes contribute independently to the pathogenesis of citrus canker.Key words: citrus canker, pathogenicity genes, transposon mutagenesis.
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Claims 12
Claim · model-written summary
XT10 and XT122 caused no visible host symptoms.
- Material
- XT10 and XT122e0
- Interactor
- Xanthomonas axonopodis pv. citri
Xanthomonas citrit0pathogen - Outcome
- did not induce any visible symptoms in the hoste0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nonpathogenic (NP) mutants XT10 and XT122, which did not induce any visible symptoms in the host”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT27 induced a callus-like lesion but no watersoaked lesion.
- Material
- XT27e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- induced a callus-like lesion but not a watersoaked lesione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XT27, which induced a callus-like lesion but not a watersoaked lesion”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT37 induced a watersoaked lesion but no callus-like eruption.
- Material
- XT37e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- was unable to induce a callus-like eruption but did induce the formation of a watersoaked lesione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XT37, which was unable to induce a callus-like eruption but did induce the formation of a watersoaked lesion around the infection site”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
The nonpathogenic mutants failed to grow in citrus tissue.
- Material
- NP mutantse0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- NP mutants failed to grow in plantae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NP mutants failed to grow in planta”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT27 and XT37 grew more slowly in citrus than wild type.
- Material
- WS - and CL - mutantse0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- growth ratee0
- Direction
- reducede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the WS - and CL - mutants showed a reduced growth rate relative to that of the wild type”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
Co-inoculation of leaves with XT27 and XT37 did not complement their defects.
- Treatment
- Co-inoculation of leaves with the WS - and CL - mutantse0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- complementation of their respective defectse0
- Direction
- did not resulte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-inoculation of leaves with the WS - and CL - mutants did not result in complementation of their respective defects”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
Mutants and wild type had similar extracellular polysaccharide, protease and amylase activities.
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- extracellular accumulation of polysaccharide, protease, and amylase activitiese0
- Direction
- similare0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extent of extracellular accumulation of polysaccharide, protease, and amylase activities by each of the mutants was similar to that of the wild type”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT27 had reduced extracellular polygalacturonate lyase activity.
- Material
- XT27e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Gene
- polygalacturonate lyasee0
- Outcome
- extracellular activity of polygalacturonate lyasee0
- Direction
- reducede0
- Comparator
- wild type and other mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extracellular activity of polygalacturonate lyase of XT27 was reduced relative to that of the wild type and other mutants”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT27 required glutamic acid for growth in culture.
- Material
- XT27e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- required glutamic acid for growth in culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XT27 also required glutamic acid for growth in culture”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
Each mutant had one transposon insertion site.
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- transposon insertion at a single sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each of the mutants resulted from transposon insertion at a single site”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT10 and XT27 had chromosomal insertion sites.
- Material
- XT10 and XT27e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- insertion sites for XT10 and XT27 were located in the chromosomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the insertion sites for XT10 and XT27 were located in the chromosome”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
Claim · model-written summary
XT37 and XT122 had insertion sites in indigenous plasmids.
- Material
- XT37 and XT122e0
- Interactor
- Xanthomonas axonopodis pv. citriXanthomonas citrit0pathogen
- Outcome
- XT37 and XT122 were located in the indigenous plasmidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those for XT37 and XT122 were located in the indigenous plasmids”
t0“citrus canker pathogen Xanthomonas axonopodis pv. citri”
The same questions in other papers
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- positive
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1 paper in the same direction as this paper · 2 in the opposite direction
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.