Abstract
We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.
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Claims 7
Claim · model-written summary
NbSEC14 silencing compromised PR-4 expression.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- NbSEC14-silencinge0
- Gene
- PR-4e0
- Outcome
- expression of defense-related PR-4e0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSEC14-silencing compromised expression of defense-related PR-4”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbSEC14 silencing compromised accumulation of JA and JA-Ile.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbSEC14-silencinge0
- Outcome
- accumulation of jasmonic acid (JA) and its derivative JA-Ilee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient NbSEC14 expression induced PR-4 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Transient expression of NbSEC14e0
- Gene
- PR-4e0
- Outcome
- PR-4 gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of NbSEC14 induced PR-4 gene expression.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbSEC14 silencing reduced avirulent R. solanacearum elicited diacylglycerol kinase and phospholipase C and D activities.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbSEC14-silencede0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Gene
- diacylglycerol kinase, phospholipase C and De0
- Outcome
- Activities of diacylglycerol kinase, phospholipase C and De0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbSEC14 silencing reduced avirulent R. solanacearum elicited diacylglycerol and phosphatidic acid synthesis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbSEC14-silencede0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Outcome
- synthesis of diacylglycerol and phosphatidic acide0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient NbSEC14 expression enhanced signaling lipid accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transient expression of NbSEC14e0
- Outcome
- Accumulation of signaling lipidse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient NbSEC14 expression enhanced activation of diacylglycerol kinase and phospholipases.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transient expression of NbSEC14e0
- Gene
- diacylglycerol kinase and phospholipasese0
- Outcome
- activation of diacylglycerol kinase and phospholipasese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14.”
t0“Nicotiana benthamiana”
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See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.