Abstract
Auxin and zinc are involved in callus and root formation in rice. However, details of the mechanism underlying this process and functional relation between zinc and auxin are unclear. In this study, proteins induced by auxin and zinc in rice were analyzed by a proteomic approach. Root formation on rice seedlings was promoted by 0.45 microM 2,4-D treatment and was further promoted by addition of 260 microM Zn. Microscopic observation revealed that the number of root primodia formed was significantly increased in 2,4-D- and Zn-treated seedlings than that of the control. A total of seven proteins, as analyzed by 2D-PAGE, were increased, and one protein was decreased by 2,4-D and Zn treatment. Expression of elongation factor-1beta' (EF-1beta') both at transcriptional and translational levels was particular abundant in callus and basal parts of young seedlings, and the accumulation of EF-1beta' was consistent with root formation induced by 2,4-D and Zn. Results indicate that higher level of EF-1beta' expression is necessary for auxin- and zinc-induced root formation in rice.
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Claims 8
Claim · model-written summary
2,4-D promoted rice seedling root formation.
- Taxon
- rice seedlings
Oryza sativae0 - Treatment
- 0.45 microM 2,4-D treatmente0
- Outcome
- Root formatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation on rice seedlings was promoted by 0.45 microM 2,4-D treatment”
Claim · model-written summary
Adding zinc further promoted root formation in 2,4-D-treated rice seedlings.
- Taxon
- rice seedlingsOryza sativae0
- Treatment
- addition of 260 microM Zne0
- Outcome
- Root formatione0
- Direction
- further promotede0
- Comparator
- 0.45 microM 2,4-D treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation on rice seedlings was promoted by 0.45 microM 2,4-D treatment and was further promoted by addition of 260 microM Zn.”
Claim · model-written summary
Combined 2,4-D and zinc treatment increased root primordia number relative to the control.
Verbatim
e0“the number of root primodia formed was significantly increased in 2,4-D- and Zn-treated seedlings than that of the control”
Claim · model-written summary
Seven proteins increased under combined 2,4-D and zinc treatment.
Not reportedTaxonComparator
Verbatim
e0“A total of seven proteins, as analyzed by 2D-PAGE, were increased, and one protein was decreased by 2,4-D and Zn treatment.”
Claim · model-written summary
One protein decreased under combined 2,4-D and zinc treatment.
Not reportedTaxonComparator
Verbatim
e0“A total of seven proteins, as analyzed by 2D-PAGE, were increased, and one protein was decreased by 2,4-D and Zn treatment.”
Claim · model-written summary
EF-1beta expression at transcriptional and translational levels was abundant in rice callus and basal regions of young seedlings.
- Organ / stage
- callus and basal parts of young seedlingse0
- Gene
- elongation factor-1beta' (EF-1beta')e0
- Outcome
- Expressione0
Not reportedTaxon
Verbatim
e0“Expression of elongation factor-1beta' (EF-1beta') both at transcriptional and translational levels was particular abundant in callus and basal parts of young seedlings”
Claim · model-written summary
EF-1beta accumulation was consistent with root formation induced by 2,4-D and zinc.
Not reportedTaxon
Verbatim
e0“the accumulation of EF-1beta' was consistent with root formation induced by 2,4-D and Zn”
Claim · model-written summary
The authors infer that higher EF-1beta expression is necessary for auxin- and zinc-induced rice root formation.
- Taxon
- riceOryza sativae0
- Treatment
- auxin- and zinc-inducede0
- Gene
- EF-1beta'e0
- Outcome
- root formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher level of EF-1beta' expression is necessary for auxin- and zinc-induced root formation in rice”
The same questions in other papers
zinc atom · growth · Oryza sativa (rice)Results differ
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- positive
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See all 33 papers →2,4-D · growth · Oryza sativa (rice)Positive and negative results
101 papers in the same direction as this paper · 5 in the opposite direction
- negative
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See all 107 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.