Abstract
Addition of 200 mm of a polyol to anthocyanin containing carrot (Daucus carota L.) cells in suspension culture decreased turgor pressure to zero and induced hyperpolarization of the membrane potential and acidification of the medium due to H(+) extrusion. These changes were shown to be slightly affected by vanadate. In parallel, a decrease in intracellular ATP and total adenylate concentrations were observed. However, when the osmoticum was NaCl acidification of the medium occurred in the absence of considerable changes in intracellular ATP concentration. These results are interpreted as indicating that a drop of turgor, by addition of a polyol, triggers a proton extrusion activity which is only slightly inhibited by vanadate but apparently ATP utilizing. The observed decrease in ATP level occurs without a change in respiration rate and is accompanied by a drop in total adenylate pool. However when NaCl is the osmoticum it is assumed that Delta(muH+) is enhanced through a Na(+)/H(+) antiporter. The difference between the two types of osmotica as related to their ability to penetrate through the cellular membrane is discussed.
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Claims 7
Claim · model-written summary
turgor pressure changed after polyol addition.
- Taxon
- carrot
Daucus carotae0 - Treatment
- 200 mm of a polyole0
- Outcome
- turgor pressuree0
- Direction
- decreasede0
- Value
- zeroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 200 mm of a polyol to anthocyanin containing carrot (Daucus carota L.) cells in suspension culture decreased turgor pressure to zero and induced hyperpolarization of the membrane potential and acidification of the medium due to H(+) extrusion.”
Claim · model-written summary
membrane potential changed after polyol addition.
- Taxon
- carrotDaucus carotae0
- Treatment
- 200 mm of a polyole0
- Outcome
- membrane potentiale0
- Direction
- hyperpolarizatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 200 mm of a polyol to anthocyanin containing carrot (Daucus carota L.) cells in suspension culture decreased turgor pressure to zero and induced hyperpolarization of the membrane potential and acidification of the medium due to H(+) extrusion.”
Claim · model-written summary
acidification of the medium changed after polyol addition.
- Taxon
- carrotDaucus carotae0
- Treatment
- 200 mm of a polyole0
- Outcome
- acidification of the mediume0
- Direction
- acidificatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 200 mm of a polyol to anthocyanin containing carrot (Daucus carota L.) cells in suspension culture decreased turgor pressure to zero and induced hyperpolarization of the membrane potential and acidification of the medium due to H(+) extrusion.”
Claim · model-written summary
In parallel, a decrease in intracellular ATP and total adenylate concentrations were observed.
- Taxon
- CarrotDaucus carotat0
- Outcome
- intracellular ATP and total adenylate concentrationse0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In parallel, a decrease in intracellular ATP and total adenylate concentrations were observed.”
t0“Carrot”
Claim · model-written summary
NaCl osmoticum caused medium acidification.
- Taxon
- CarrotDaucus carotat0
- Treatment
- NaCle0
- Outcome
- acidification of the mediume0
- Direction
- acidificatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, when the osmoticum was NaCl acidification of the medium occurred in the absence of considerable changes in intracellular ATP concentration.”
t0“Carrot”
Claim · model-written summary
Intracellular ATP showed no considerable change under NaCl osmoticum.
- Taxon
- CarrotDaucus carotat0
- Treatment
- NaCle0
- Outcome
- intracellular ATP concentratione0
- Direction
- absence of considerable changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, when the osmoticum was NaCl acidification of the medium occurred in the absence of considerable changes in intracellular ATP concentration.”
t0“Carrot”
Claim · model-written summary
Respiration rate did not change during the ATP decrease.
- Taxon
- CarrotDaucus carotat0
- Outcome
- respiration ratee0
- Direction
- without a changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The observed decrease in ATP level occurs without a change in respiration rate and is accompanied by a drop in total adenylate pool.”
t0“Carrot”
The same questions in other papers
sodium chloride · molecular: metabolites · Daucus carota (carrot)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.