Abstract
Protein content and activities of the enzymes glutamine synthetase (EC 6.3.1.2), NADH‐glutamate synthase (EC 1.4.1.14), NADH‐glutamate dehydrogenase (reductive amination (EC 1.4.1.2) and NAD + ‐glutamate dehydrogenase (oxidative deamination) (EC 1.4.1.2) from the plant fraction of root nodules of alfalfa ( Medicago sativa L. cv. Aragon) were determined under water stress. Only NADH‐glutamate synthase activity was inhibited during drought. The results indicate that the glutamine synthetase/NADH‐glutamate synthase cycle was fully operational in alfalfa nodules of control or even mildly stressed plants when N 2 ‐fixation was not inhibited, but that the coupling between glutamine synthetase and NADH‐glutamate synthase was lost as drought progressed. Patterns of glutamine synthetase and NADH‐/NAD + ‐gluta‐mate dehydrogenase activities reflect changes in ammonia content of nodules and/or availability of carbon substrates, and indicate that nodules maintain sufficient enzyme activity for ammonia assimilation throughout water stress.
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Claims 5
Claim · model-written summary
Drought inhibited only NADH-glutamate synthase activity among the tested enzymes.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- droughte0
- Gene
- NADH‐glutamate synthasee0
- Outcome
- NADH‐glutamate synthase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only NADH‐glutamate synthase activity was inhibited during drought.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
The glutamine synthetase/NADH-glutamate synthase cycle remained operational in control and mildly stressed nodules when nitrogen fixation was not inhibited.
- Taxon
- alfalfaMedicago sativae0
- Organ / stage
- nodulese0
- Gene
- glutamine synthetase/NADH‐glutamate synthasee0
- Outcome
- fully operationale0
- Qualifier
- when N 2 ‐fixation was not inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the glutamine synthetase/NADH‐glutamate synthase cycle was fully operational in alfalfa nodules of control or even mildly stressed plants when N 2 ‐fixation was not inhibited”
Claim · model-written summary
Coupling between glutamine synthetase and NADH-glutamate synthase was lost as drought progressed.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- droughte0
- Outcome
- coupling between glutamine synthetase and NADH‐glutamate synthasee0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the coupling between glutamine synthetase and NADH‐glutamate synthase was lost as drought progressed.”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Enzyme activity patterns reflect changes in nodule ammonia content or carbon substrate availability.
- Taxon
- alfalfaMedicago sativat0
- Compound
- ammoniae0
- Outcome
- glutamine synthetase and NADH‐/NAD + ‐gluta‐mate dehydrogenase activitiese0
- Qualifier
- and/or availability of carbon substratese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Patterns of glutamine synthetase and NADH‐/NAD + ‐gluta‐mate dehydrogenase activities reflect changes in ammonia content of nodules and/or availability of carbon substrates”
t0“alfalfa (Medicago sativa)”
Claim · model-written summary
Nodules maintain sufficient enzyme activity for ammonia assimilation throughout water stress.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- nodulese0
- Treatment
- water stresse0
- Outcome
- sufficient enzyme activity for ammonia assimilatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nodules maintain sufficient enzyme activity for ammonia assimilation throughout water stress.”
t0“alfalfa (Medicago sativa)”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.