Abstract
Abstract The microbiological method for the determination of folate in plant foods uses the growth response of folate-dependent Lactobacillus rhamnosus in extracts that have been enzymatically treated to release the bound vitamin. However, the use of cryoprotected cultures is hampered by low recovery of the microorganism after extended frozen storage times. In this study, growth of L. rhamnosus was enhanced using a microaerophilic enrichment procedure and optimal pH conditions and enzyme reaction times were determined for the release of bound folate in spinach. Optimum pH values for the release of bound folate in spinach samples treated with α-amylase or protease were 3.0 and 4.0, respectively. Although treatment with α-amylase had no significant (P > 0.05) effect on measured folate, addition of protease at pH 4.0 significantly (P ≤ 0.05) increased the release of folate at an optimum incubation time of 8 h. Therefore, a dual-enzyme treatment (protease and conjugase) is sufficient to determine folate content in spinach.
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Claims 5
Claim · model-written summary
Optimal pH for alpha-amylase release of bound spinach folate was 3.0.
- Taxon
- spinach
Spinacia oleraceae0 - Treatment
- α-amylasee0
- Outcome
- release of bound folatee0
- Value
- 3.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum pH values for the release of bound folate in spinach samples treated with α-amylase or protease were 3.0 and 4.0, respectively.”
Claim · model-written summary
Optimal pH for protease release of bound spinach folate was 4.0.
- Taxon
- spinachSpinacia oleraceae0
- Treatment
- proteasee0
- Outcome
- release of bound folatee0
- Value
- 4.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum pH values for the release of bound folate in spinach samples treated with α-amylase or protease were 3.0 and 4.0, respectively.”
Claim · model-written summary
Alpha-amylase had no significant effect on measured folate.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- α-amylasee0
- Outcome
- measured folatee0
- Direction
- no significante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with α-amylase had no significant (P > 0.05) effect on measured folate”
t0“spinach”
Claim · model-written summary
Protease at pH 4 significantly increased folate release at eight hours.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- protease at pH 4.0e0
- Outcome
- release of folatee0
- Direction
- increasede0
- Value
- 8 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“addition of protease at pH 4.0 significantly (P ≤ 0.05) increased the release of folate at an optimum incubation time of 8 h.”
t0“spinach”
Claim · model-written summary
Protease and conjugase treatment was judged sufficient for spinach folate determination.
- Taxon
- spinachSpinacia oleraceae0
- Outcome
- a dual-enzyme treatment (protease and conjugase) is sufficient to determine folate contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a dual-enzyme treatment (protease and conjugase) is sufficient to determine folate content in spinach.”
The same questions in other papers
ph · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.