Abstract
Several RNA silencing pathways in plants restrict viral infections and are suppressed by distinct viral proteins. Here we show that the endogenous trans-acting (ta)siRNA pathway, which depends on Dicer-like (DCL) 4 and RNA-dependent RNA polymerase (RDR) 6, is suppressed by infection of Arabidopsis with Cauliflower mosaic virus (CaMV). This effect was associated with overaccumulation of unprocessed, RDR6-dependent precursors of tasiRNAs and is due solely to expression of the CaMV transactivator/viroplasmin (TAV) protein. TAV expression also impaired secondary, but not primary, siRNA production from a silenced transgene and increased accumulation of mRNAs normally silenced by the four known tasiRNA families and RDR6-dependent secondary siRNAs. Moreover, TAV expression upregulated DCL4, DRB4 and AGO7 that mediate tasiRNA biogenesis. Our findings suggest that TAV is a general inhibitor of silencing amplification that impairs DCL4-mediated processing of RDR6-dependent double-stranded RNA to siRNAs. The resulting deficiency in tasiRNAs and other RDR6-/DCL4-dependent siRNAs appears to trigger a feedback mechanism that compensates for the inhibitory effects.
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Claims 9
Claim · model-written summary
CaMV infection suppressed the Arabidopsis tasiRNA pathway.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- infection of Arabidopsis with Cauliflower mosaic virus (CaMV)e0
- Interactor
- Cauliflower mosaic virus (CaMV)
Cauliflower mosaic viruse0pathogen - Outcome
- trans-acting (ta)siRNA pathwaye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“the endogenous trans-acting (ta)siRNA pathway, which depends on Dicer-like (DCL) 4 and RNA-dependent RNA polymerase (RDR) 6, is suppressed by infection of Arabidopsis with Cauliflower mosaic virus (CaMV)”
Claim · model-written summary
Suppression was associated with excess unprocessed tasiRNA precursors.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RDR6e0
- Outcome
- unprocessed, RDR6-dependent precursors of tasiRNAse0
- Direction
- overaccumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“This effect was associated with overaccumulation of unprocessed, RDR6-dependent precursors of tasiRNAs”
Claim · model-written summary
CaMV TAV alone caused tasiRNA pathway suppression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CaMV transactivator/viroplasmin (TAV) proteine0
- Outcome
- is due solely to expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“is due solely to expression of the CaMV transactivator/viroplasmin (TAV) protein”
Claim · model-written summary
TAV expression impaired secondary siRNA production from a silenced transgene.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TAV expressione0
- Outcome
- secondary, but not primary, siRNA productione0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“TAV expression also impaired secondary, but not primary, siRNA production from a silenced transgene”
Claim · model-written summary
TAV expression spared primary siRNA production from the silenced transgene.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TAV expressione0
- Outcome
- primary, siRNA productione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“TAV expression also impaired secondary, but not primary, siRNA production from a silenced transgene”
Claim · model-written summary
TAV expression increased mRNAs normally silenced by tasiRNAs and secondary siRNAs.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TAV expressione0
- Gene
- RDR6e0
- Outcome
- accumulation of mRNAse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“TAV expression also impaired secondary, but not primary, siRNA production from a silenced transgene and increased accumulation of mRNAs normally silenced by the four known tasiRNA families and RDR6-dependent secondary siRNAs”
Claim · model-written summary
TAV expression upregulated DCL4, DRB4 and AGO7.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- TAV expressione0
- Gene
- DCL4, DRB4 and AGO7e0
- Outcome
- DCL4, DRB4 and AGO7e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“TAV expression upregulated DCL4, DRB4 and AGO7 that mediate tasiRNA biogenesis”
Claim · model-written summary
The authors propose TAV inhibits silencing amplification through impaired DCL4 processing of RDR6 double-stranded RNA.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- DCL4e0
- Outcome
- silencing amplificatione0
- Qualifier
- impairs DCL4-mediated processing of RDR6-dependent double-stranded RNA to siRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“TAV is a general inhibitor of silencing amplification that impairs DCL4-mediated processing of RDR6-dependent double-stranded RNA to siRNAs”
Claim · model-written summary
The authors propose deficient RDR6/DCL4 siRNAs trigger compensatory feedback.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RDR6-/DCL4-dependent siRNAse0
- Outcome
- feedback mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis”
e0“The resulting deficiency in tasiRNAs and other RDR6-/DCL4-dependent siRNAs appears to trigger a feedback mechanism that compensates for the inhibitory effects”
The same questions in other papers
Brassica oleracea var. botrytis · Cauliflower mosaic virus · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
infection · Cauliflower mosaic virus · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.