Abstract
Transformed Arabidopsis plants were used to study the effect of the cauliflower mosaic virus (CaMV) inclusion body protein on translation of dicistronic RNA. Reporter plants contain a dicistronic transcription unit with CaMV open reading frame VII (ORF VII) as the first and the [beta]-glucuronidase (GUS) reporter ORF as the second cistron. "Transactivator plants" contain CaMV ORF VI under the control of the strong CaMV 35S promoter. The transactivator plants were difficult to regenerate and showed an abnormal phenotype. Expression of GUS activity in the reporter plants was very low, but high GUS activity could be induced by introduction of gene VI, either by crossing with plants containing gene VI as a transgene or by infection with CaMV. Histological GUS assays showed that transactivation occurred in all types of tissue and at all developmental stages. The practical implications of the induction of GUS expression from the dicistronic unit by virus infection are discussed.
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Claims 7
Claim · model-written summary
Transactivator Arabidopsis plants were difficult to regenerate.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- difficult to regeneratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“The transactivator plants were difficult to regenerate and showed an abnormal phenotype”
Claim · model-written summary
Transactivator Arabidopsis plants showed an abnormal phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- abnormal phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“The transactivator plants were difficult to regenerate and showed an abnormal phenotype”
Claim · model-written summary
Reporter plants had very low GUS activity before gene VI introduction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- very lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“Expression of GUS activity in the reporter plants was very low”
Claim · model-written summary
Crossing with gene VI transgenic plants induced high GUS activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- crossing with plants containing gene VI as a transgenee0
- Gene
- gene VIe0
- Outcome
- GUS activitye0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“high GUS activity could be induced by introduction of gene VI, either by crossing with plants containing gene VI as a transgene or by infection with CaMV”
Claim · model-written summary
CaMV infection induced high GUS activity in reporter plants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- infection with CaMVe0
- Interactor
- CaMV
Cauliflower mosaic viruse1pathogen - Gene
- gene VIe0
- Outcome
- GUS activitye0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“high GUS activity could be induced by introduction of gene VI, either by crossing with plants containing gene VI as a transgene or by infection with CaMV”
e1“cauliflower mosaic virus (CaMV)”
Claim · model-written summary
Transactivation occurred across all tissue types.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- transactivation occurred in all types of tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“transactivation occurred in all types of tissue and at all developmental stages”
Claim · model-written summary
Transactivation occurred at all developmental stages.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- transactivation occurred in all types of tissue and at all developmental stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Plants”
e0“transactivation occurred in all types of tissue and at all developmental stages”
The same questions in other papers
Brassica oleracea var. botrytis · Cauliflower mosaic virus · ArabidopsisPositive and negative results
2 in the opposite direction
- no effect
- negative
infection · Cauliflower mosaic virus · ArabidopsisPositive and negative results
3 in the opposite direction
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.