Abstract
Mutations have been inserted into the virion and complementary sense ORFs encoding proteins with M(r)s in excess of 9 kDa of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV). Wild-type and mutant monomeric clones were tested for their ability to replicate, produce PYMV-specific DNA, spread and cause symptoms in Nicotiana benthamiana plants following biolistic inoculation. Dimeric clones of the DNA A mutants were also investigated by agroinoculation of leaf discs. In contrast to N. benthamiana plants agroinoculated with PYMV DNA A, in which the wild-type DNA A component was capable of limited independent replication and spread, both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated by the biolistic method. Mixtures of both genomic components were also infectious for potato plants following biolistic inoculation. Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants. However, the AL2 mutation, but not the AL1 mutation, allowed viral DNA replication in leaf discs. Mutations to both the AR1 and AL3 ORFs produced clones which were infectious in plants but showed a considerable delay in the production of attenuated symptoms as compared to wild-type infections. Mutating the AL3 ORF dramatically reduced viral DNA replication in both whole plants and leaf discs. Mutations to the AL4 ORF produced clones which were as infectious for both N. benthamiana and potato plants as the wild-type clones. Our results are compared with those from mutagenesis studies on related bipartite geminiviruses.
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Claims 9
Claim · model-written summary
Mutations in ORFs AL1, AL2, BR1 and BL1 made clones incapable of infecting N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae1 - Treatment
- Mutations in ORFs AL1, AL2, BR1 and BL1e1
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- infecting N. benthamiana plantse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
Claim · model-written summary
The AL2 mutation, unlike the AL1 mutation, allowed viral DNA replication in leaf discs.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- the AL2 mutatione2
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- viral DNA replicatione2
- Direction
- allowede2
- Comparator
- the AL1 mutatione2
- Setting
- leaf discse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
e2“However, the AL2 mutation, but not the AL1 mutation, allowed viral DNA replication in leaf discs”
Claim · model-written summary
Mutations to the AR1 and AL3 ORFs gave infectious clones with a considerable delay in attenuated symptoms compared with wild-type infections.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- Mutations to both the AR1 and AL3 ORFse3
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- production of attenuated symptomse3
- Direction
- considerable delaye3
- Comparator
- wild-type infectionse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
e3“Mutations to both the AR1 and AL3 ORFs produced clones which were infectious in plants but showed a considerable delay in the production of attenuated symptoms as compared to wild-type infections”
Claim · model-written summary
Mutating the AL3 ORF dramatically reduced viral DNA replication in whole plants and leaf discs.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- Mutating the AL3 ORFe4
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- viral DNA replicatione4
- Direction
- dramatically reducede4
- Setting
- whole plants and leaf discse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
e4“Mutating the AL3 ORF dramatically reduced viral DNA replication in both whole plants and leaf discs”
Claim · model-written summary
Mutations to the AL4 ORF gave clones as infectious in N. benthamiana as the wild-type clones.
- Taxon
- N. benthamianaNicotiana benthamianae5
- Treatment
- Mutations to the AL4 ORFe5
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- infectiouse5
- Direction
- as infectiouse5
- Comparator
- wild-type clonese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e5“Mutations to the AL4 ORF produced clones which were as infectious for both N. benthamiana and potato plants as the wild-type clones”
Claim · model-written summary
Mutations to the AL4 ORF gave clones as infectious in potato as the wild-type clones.
- Taxon
- potato
Solanum tuberosume5 - Treatment
- Mutations to the AL4 ORFe5
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- infectiouse5
- Direction
- as infectiouse5
- Comparator
- wild-type clonese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e5“Mutations to the AL4 ORF produced clones which were as infectious for both N. benthamiana and potato plants as the wild-type clones”
Claim · model-written summary
Mixtures of both genomic components were infectious for potato plants after biolistic inoculation.
- Taxon
- potatoSolanum tuberosume6
- Treatment
- Mixtures of both genomic componentse6
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- infectiouse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e6“Mixtures of both genomic components were also infectious for potato plants following biolistic inoculation”
Claim · model-written summary
The wild-type DNA A component alone was capable of limited independent replication and spread in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- wild-type DNA A componente7
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- independent replication and spreade7
- Qualifier
- limitede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
e7“in which the wild-type DNA A component was capable of limited independent replication and spread”
Claim · model-written summary
Both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated biolistically.
- Taxon
- plantsViridiplantaee0
- Treatment
- both excised DNA A and B componentse0
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- DNA replication and symptom developmente0
- Direction
- requirede0
Not reportedComparator
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e0“both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated by the biolistic method”
The same questions in other papers
loss of function · organism · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
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10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.