Claim · model-written summary
Mutations in ORFs AL1, AL2, BR1 and BL1 made clones incapable of infecting N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae1 - Treatment
- Mutations in ORFs AL1, AL2, BR1 and BL1e1
- Interactor
- potato yellow mosaic geminivirust0pathogen
- Outcome
- infecting N. benthamiana plantse1
Not reportedMaterialGeneCompoundOrgan / stageSecond interactorSecond interactor roleComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mutational analysis of potato yellow mosaic geminivirus”
e1“Mutations in ORFs AL1, AL2, BR1 and BL1 resulted in clones incapable of infecting N. benthamiana plants”
Title
Mutational analysis of potato yellow mosaic geminivirus
Y. K. Sung; R. H. A. Coutts Journal of General Virology 1995. doi:10.1099/0022-1317-76-7-1773
Other claims from this paper (8)
- Intervention effect The AL2 mutation, unlike the AL1 mutation, allowed viral DNA replication in leaf discs.
- Intervention effect Mutations to the AR1 and AL3 ORFs gave infectious clones with a considerable delay in attenuated symptoms compared with wild-type infections.
- Intervention effect Mutating the AL3 ORF dramatically reduced viral DNA replication in whole plants and leaf discs.
- Intervention effect Mutations to the AL4 ORF gave clones as infectious in N. benthamiana as the wild-type clones.
- Intervention effect Mutations to the AL4 ORF gave clones as infectious in potato as the wild-type clones.
- Observation Mixtures of both genomic components were infectious for potato plants after biolistic inoculation.
- Observation The wild-type DNA A component alone was capable of limited independent replication and spread in N. benthamiana.
- Intervention effect Both excised DNA A and B components were required for DNA replication and symptom development in plants inoculated biolistically.