Abstract
Ubiquitin like protein 5 (UBL5) interacts with other proteins to regulate their function but differs from ubiquitin and other UBLs because it does not form covalent conjugates. Ubiquitin and most UBLs mediate the degradation of target proteins through the 26S proteasome but it is not known if UBL5 can also do that. Here we found that the UBL5s of rice and Nicotiana benthamiana interacted with rice stripe virus (RSV) p3 protein. Silencing of NbUBL5s in N. benthamiana facilitated RSV infection, while UBL5 overexpression conferred resistance to RSV in both N. benthamiana and rice. Further analysis showed that NbUBL5.1 impaired the function of p3 as a suppressor of silencing by degrading it through the 26S proteasome. NbUBL5.1 and OsUBL5 interacted with RPN10 and RPN13, the receptors of ubiquitin in the 26S proteasome. Furthermore, silencing of NbRPN10 or NbRPN13 compromised the degradation of p3 mediated by NbUBL5.1. Together, the results suggest that UBL5 mediates the degradation of RSV p3 protein through the 26S proteasome, a previously unreported plant defense strategy against RSV infection.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
UBL5 proteins from rice and Nicotiana benthamiana interacted with RSV p3.
- Taxon
- rice
Oryza sativae0 - Interactor
- rice stripe virusTenuivirus oryzaclavataee0
- Gene
- UBL5se0
- Outcome
- interacted with rice stripe virus (RSV) p3 proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBL5s of rice and Nicotiana benthamiana interacted with rice stripe virus (RSV) p3 protein”
Claim · model-written summary
Silencing NbUBL5s facilitated RSV infection in Nicotiana benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- Silencing of NbUBL5se0
- Interactor
- RSVe0
- Gene
- NbUBL5se0
- Outcome
- RSV infectione0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbUBL5s in N. benthamiana facilitated RSV infection”
Claim · model-written summary
UBL5 overexpression conferred RSV resistance in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- UBL5 overexpressione0
- Interactor
- RSVe0
- Gene
- UBL5e0
- Outcome
- resistance to RSVe0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBL5 overexpression conferred resistance to RSV in both N. benthamiana and rice”
Claim · model-written summary
UBL5 overexpression conferred RSV resistance in rice.
- Taxon
- riceOryza sativae0
- Treatment
- UBL5 overexpressione0
- Interactor
- RSVe0
- Gene
- UBL5e0
- Outcome
- resistance to RSVe0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UBL5 overexpression conferred resistance to RSV in both N. benthamiana and rice”
Claim · model-written summary
NbUBL5.1 impaired the silencing suppressor activity of p3 by proteasomal degradation.
Not reportedTaxonInteractor
Verbatim
e0“NbUBL5.1 impaired the function of p3 as a suppressor of silencing by degrading it through the 26S proteasome”
Claim · model-written summary
NbUBL5.1 and OsUBL5 interacted with proteasome receptors RPN10 and RPN13.
Not reportedTaxon
Verbatim
e0“NbUBL5.1 and OsUBL5 interacted with RPN10 and RPN13, the receptors of ubiquitin in the 26S proteasome”
Claim · model-written summary
Silencing NbRPN10 compromised NbUBL5.1-mediated p3 degradation.
Not reportedTaxonComparator
Verbatim
e0“silencing of NbRPN10 or NbRPN13 compromised the degradation of p3 mediated by NbUBL5.1”
Claim · model-written summary
Silencing NbRPN13 compromised NbUBL5.1-mediated p3 degradation.
- Treatment
- silencing of NbRPN10 or NbRPN13e0
- Gene
- NbRPN13e0
- Outcome
- degradation of p3e0
- Direction
- compromisede0
Not reportedTaxonComparator
Verbatim
e0“silencing of NbRPN10 or NbRPN13 compromised the degradation of p3 mediated by NbUBL5.1”
The same questions in other papers
loss of function · organism · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.