Abstract
The unfolded protein response (UPR) plays important roles in plant virus infection. Our previous study has proved that rice stripe virus (RSV) infection elicits host UPR. However, the mechanism on how the UPR is triggered upon RSV infection remains obscure. Here, we show that the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana. We found that membrane-associated proteins NSvc2 and NSvc4 encoded by RSV are responsible for the activation of the bZIP17/28 branch. Ectopic expression of NSvc2 or NSvc4 in plant leaves induced the proteolytic processing of NbbZIP17/28 and up-regulated the expression of UPR-related genes. Silencing NbbZIP17/28 significantly inhibited RSV infection. We show that RSV can specifically elicit the UPR through the bZIP17/28 branch, thus promoting virus infection of N. benthamiana plants.
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Claims 6
Claim · model-written summary
RSV infection activates the bZIP17/28 branch of the UPR signalling pathway in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- RSV infectione1
- Interactor
- RSVe1pathogen
- Outcome
- the bZIP17/28 branch of the UPR signalling pathwaye1
- Direction
- activatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
Claim · model-written summary
The RSV proteins NSvc2 and NSvc4 are responsible for activation of the bZIP17/28 branch.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- RSVe2pathogen
- Gene
- NSvc2 and NSvc4e2
- Outcome
- activation of the bZIP17/28 branche2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
e2“membrane-associated proteins NSvc2 and NSvc4 encoded by RSV are responsible for the activation of the bZIP17/28 branch”
Claim · model-written summary
Ectopic expression of NSvc2 or NSvc4 induced proteolytic processing of NbbZIP17/28 in Nicotiana benthamiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Ectopic expression of NSvc2 or NSvc4e3
- Gene
- NbbZIP17/28e3
- Outcome
- proteolytic processing of NbbZIP17/28e3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
e3“Ectopic expression of NSvc2 or NSvc4 in plant leaves induced the proteolytic processing of NbbZIP17/28 and up-regulated the expression of UPR-related genes”
Claim · model-written summary
Ectopic expression of NSvc2 or NSvc4 up-regulated the expression of UPR-related genes in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Ectopic expression of NSvc2 or NSvc4e3
- Outcome
- expression of UPR-related genese3
- Direction
- up-regulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
e3“Ectopic expression of NSvc2 or NSvc4 in plant leaves induced the proteolytic processing of NbbZIP17/28 and up-regulated the expression of UPR-related genes”
Claim · model-written summary
Silencing NbbZIP17/28 significantly inhibited RSV infection in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Silencing NbbZIP17/28e4
- Interactor
- RSVe4pathogen
- Gene
- NbbZIP17/28e4
- Outcome
- RSV infectione4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
e4“Silencing NbbZIP17/28 significantly inhibited RSV infection”
Claim · model-written summary
RSV promotes its own infection by specifically eliciting the UPR through the bZIP17/28 branch.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- RSVe5pathogen
- Outcome
- virus infectione5
- Qualifier
- RSV can specifically elicit the UPR through the bZIP17/28 branche5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the bZIP17/28 branch of the UPR signalling pathway is activated upon RSV infection in Nicotiana benthamiana”
e5“We show that RSV can specifically elicit the UPR through the bZIP17/28 branch, thus promoting virus infection of N. benthamiana plants”
The same questions in other papers
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See all 14 papers →infection · quality: protein · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.