Abstract
Pattern-triggered immunity (PTI) is broad spectrum and manipulation of PTI is believed to represent an attractive way to engineer plants with broad-spectrum disease resistance. PTI is activated upon perception of microbe-associated molecular patterns (MAMPs) by pattern-recognition receptors (PRRs). We have recently demonstrated that the L-type lectin receptor kinase-VI.2 (LecRK-VI.2) positively regulates Arabidopsis thaliana PTI. Here we show through in vitro pull-down, bimolecular fluorescence complementation and co-immunoprecipitation analyses that LecRK-VI.2 associates with the PRR FLS2. We also demonstrated that LecRK-VI.2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plant Nicotiana benthamiana. Wild tobacco plants ectopically expressing LecRK-VI.2 were indeed more resistant to virulent hemi-biotrophic and necrotrophic bacteria, but not to the fungal pathogen Botrytis cinerea suggesting that, as with Arabidopsis, the LecRK-VI.2 protective effect in N. benthamiana is bacteria specific. Ectopic expression of LecRK-VI.2 in N. benthamiana primed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin. Our findings identified LecRK-VI.2 as a member of the FLS2 receptor complex and suggest that heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria.
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Claims 8
Claim · model-written summary
LecRK-VI.2 associated with FLS2.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- LecRK-VI.2e0
- Outcome
- associates with the PRR FLS2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LecRK-VI.2 associates with the PRR FLS2”
t0“Arabidopsis”
Claim · model-written summary
LecRK-VI.2 remained functional after transfer from Arabidopsis to Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- LecRK-VI.2e0
- Outcome
- remains functionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LecRK-VI.2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plant Nicotiana benthamiana”
Claim · model-written summary
Wild tobacco expressing LecRK-VI.2 was more resistant to virulent bacteria.
- Taxon
- Wild tobacco
_(Solanaceae)_Plant.JPG?width=60)
Nicotiana rusticae0 - Treatment
- ectopically expressing LecRK-VI.2e0
- Outcome
- resistant to virulent hemi-biotrophic and necrotrophic bacteriae0
- Direction
- more resistante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Solanaceae)_Plant.JPG?width=500)
Verbatim
e0“Wild tobacco plants ectopically expressing LecRK-VI.2 were indeed more resistant to virulent hemi-biotrophic and necrotrophic bacteria”
Claim · model-written summary
Wild tobacco expressing LecRK-VI.2 did not gain resistance to Botrytis cinerea.
- Taxon
- Wild tobaccoNicotiana rusticae1
- Treatment
- ectopically expressing LecRK-VI.2e1
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- fungal pathogen Botrytis cinereae0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not to the fungal pathogen Botrytis cinerea”
e1“Wild tobacco plants ectopically expressing LecRK-VI.2”
Claim · model-written summary
Ectopic LecRK-VI.2 expression primed reactive oxygen species production and MAPK activity after flagellin treatment.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Ectopic expression of LecRK-VI.2e0
- Outcome
- reactive oxygen species production, mitogen-activated protein kinase (MAPK) activitye0
- Direction
- primede0
- Qualifier
- upon treatment with the MAMP flagelline0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of LecRK-VI.2 in N. benthamiana primed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin”
Claim · model-written summary
Ectopic LecRK-VI.2 expression primed callose deposition and gene expression after flagellin treatment.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Ectopic expression of LecRK-VI.2e0
- Outcome
- callose deposition and gene expressione0
- Direction
- primede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of LecRK-VI.2 in N. benthamiana primed PTI-mediated reactive oxygen species production, mitogen-activated protein kinase (MAPK) activity, callose deposition and gene expression upon treatment with the MAMP flagellin”
Claim · model-written summary
The authors identify LecRK-VI.2 as a member of the FLS2 receptor complex.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- LecRK-VI.2e0
- Outcome
- member of the FLS2 receptor complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified LecRK-VI.2 as a member of the FLS2 receptor complex”
t0“Arabidopsis”
Claim · model-written summary
The authors suggest expressing PRR-complex components across species to engineer bacterial disease resistance.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Outcome
- heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria”
t0“Nicotiana benthamiana”
The same questions in other papers
overexpression · molecular: expression · Nicotiana benthamianaPositive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.