Abstract
In interactions between poleroviruses and their hosts, few cellular proteins have been identified that directly interact with the multifunctional virus P0 protein. To help explore the functions of P0, we identified a Brassica yellows virus genotype A (BrYV-A) P0BrA-interacting protein from Nicotiana benthamiana, Rubisco assembly factor 2 (NbRAF2), which localizes in the nucleus, cell periphery, chloroplasts, and stromules. We found that its C-terminal domain (amino acids 183-211) is required for self-interaction. A split ubiquitin membrane-bound yeast two-hybrid system and co-immunoprecipitation assays showed that NbRAF2 interacted with P0BrA, and co-localized in the nucleus and at the cell periphery. Interestingly, the nuclear pool of NbRAF2 decreased in the presence of P0BrA and during BrYV-A infection, and the P0BrA-mediated reduction of nuclear NbRAF2 required dual localization of NbRAF2 in the chloroplasts and nucleus. Tobacco rattle virus-based virus-induced gene silencing of NbRAF2 promoted BrYV-A infection in N. benthamiana, and the overexpression of nuclear NbRAF2 inhibited BrYV-A accumulation. Potato leafroll virus P0PL also interacted with NbRAF2 and decreased its nuclear accumulation, indicating that NbRAF2 may be a common target of poleroviruses. These results suggest that nuclear NbRAF2 possesses antiviral activity against BrYV-A infection, and that BrYV-A P0BrA interacts with NbRAF2 and alters its localization pattern to facilitate virus infection.
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Claims 10
Claim · model-written summary
NbRAF2 interacted with the Brassica yellows virus P0 protein (P0BrA) in split ubiquitin and co-immunoprecipitation assays.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat1 - Interactor
- Brassica yellows virusBrassica yellows virust0pathogen
- Gene
- NbRAF2e1
- Outcome
- interacted with P0BrAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A split ubiquitin membrane-bound yeast two-hybrid system and co-immunoprecipitation assays showed that NbRAF2 interacted with P0BrA”
t0“Brassica yellows virus P0 protein”
t1“Nicotiana benthamiana”
Claim · model-written summary
The C-terminal domain (amino acids 183-211) of NbRAF2 is required for its self-interaction.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Outcome
- self-interactione1
- Qualifier
- C-terminal domain (amino acids 183-211)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We found that its C-terminal domain (amino acids 183-211) is required for self-interaction”
t1“Nicotiana benthamiana”
Claim · model-written summary
The nuclear pool of NbRAF2 decreased in the presence of P0BrA and during BrYV-A infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- in the presence of P0BrAe1
- Interactor
- BrYV-Ae1pathogen
- Gene
- NbRAF2e1
- Outcome
- nuclear pool of NbRAF2e1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the nuclear pool of NbRAF2 decreased in the presence of P0BrA and during BrYV-A infection”
t1“Nicotiana benthamiana”
Claim · model-written summary
The P0BrA-mediated reduction of nuclear NbRAF2 required dual localization of NbRAF2 in the chloroplasts and nucleus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Gene
- NbRAF2e2
- Outcome
- reduction of nuclear NbRAF2e2
- Qualifier
- dual localization of NbRAF2 in the chloroplasts and nucleuse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“the P0BrA-mediated reduction of nuclear NbRAF2 required dual localization of NbRAF2 in the chloroplasts and nucleus”
t1“Nicotiana benthamiana”
Claim · model-written summary
Tobacco rattle virus-based silencing of NbRAF2 promoted BrYV-A infection in N. benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- Tobacco rattle virus-based virus-induced gene silencing of NbRAF2e3
- Interactor
- BrYV-Ae3pathogen
- Gene
- NbRAF2e3
- Outcome
- BrYV-A infectione3
- Direction
- promotede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Tobacco rattle virus-based virus-induced gene silencing of NbRAF2 promoted BrYV-A infection in N. benthamiana”
t1“Nicotiana benthamiana”
Claim · model-written summary
Overexpression of nuclear NbRAF2 inhibited BrYV-A accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- overexpression of nuclear NbRAF2e4
- Interactor
- BrYV-Ae4pathogen
- Gene
- NbRAF2e4
- Outcome
- BrYV-A accumulatione4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the overexpression of nuclear NbRAF2 inhibited BrYV-A accumulation”
t1“Nicotiana benthamiana”
Claim · model-written summary
Potato leafroll virus P0PL interacted with NbRAF2.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Interactor
- Potato leafroll virus
Potato leafroll viruse5pathogen - Gene
- NbRAF2e5
- Outcome
- interacted with NbRAF2e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Potato leafroll virus P0PL also interacted with NbRAF2 and decreased its nuclear accumulation”
t1“Nicotiana benthamiana”
Claim · model-written summary
Potato leafroll virus P0PL decreased the nuclear accumulation of NbRAF2.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- Potato leafroll virus P0PLe5
- Interactor
- Potato leafroll virusPotato leafroll viruse5pathogen
- Outcome
- nuclear accumulatione5
- Direction
- decreasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Potato leafroll virus P0PL also interacted with NbRAF2 and decreased its nuclear accumulation”
t1“Nicotiana benthamiana”
Claim · model-written summary
Nuclear NbRAF2 possesses antiviral activity against BrYV-A infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Interactor
- BrYV-Ae6pathogen
- Gene
- NbRAF2e6
- Outcome
- antiviral activity against BrYV-A infectione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These results suggest that nuclear NbRAF2 possesses antiviral activity against BrYV-A infection”
t1“Nicotiana benthamiana”
Claim · model-written summary
BrYV-A P0BrA interacts with NbRAF2 and alters its localization pattern to facilitate virus infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Interactor
- BrYV-Ae7pathogen
- Gene
- NbRAF2e7
- Outcome
- virus infectione7
- Qualifier
- alters its localization patterne7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“BrYV-A P0BrA interacts with NbRAF2 and alters its localization pattern to facilitate virus infection”
t1“Nicotiana benthamiana”
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- negative
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10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.