Abstract
The ubiquitin-26S proteasome system (UPS) is a conserved protein degradation process involved in plant growth and immunity. However, whether some UPS E3 ligases directly target plant viruses in the endoplasmic reticulum (ER) remains less understood. Here, we identify an E3 ubiquitin ligase Hmg-CoA reductase degradation 1 of Nicotiana benthamiana (NbHRD1) interacting with the triple gene block (TGB) movement proteins of beet necrotic yellow vein virus (BNYVV) in the ER. The TGB proteins are ubiquitinated by NbHRD1 and then degraded by the UPS. Consequently, overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana. Moreover, NbHRD1a mainly impairs BNYVV cell-to-cell movement, rather than virus replication. Interestingly, NbHRD1 also targets the TGB proteins of potato virus X for ubiquitination and virus inhibition. Collectively, our results demonstrate that NbHRD1 is an important antiviral component targeting plant viruses with TGB movement proteins.
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Claims 6
Claim · model-written summary
NbHRD1 of Nicotiana benthamiana interacts with the TGB movement proteins of BNYVV in the endoplasmic reticulum.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Interactor
- beet necrotic yellow vein virus (BNYVV)
Beet necrotic yellow vein viruse1pathogen - Gene
- NbHRD1e1
- Outcome
- interacting with the triple gene block (TGB) movement proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we identify an E3 ubiquitin ligase Hmg-CoA reductase degradation 1 of Nicotiana benthamiana (NbHRD1) interacting with the triple gene block (TGB) movement proteins of beet necrotic yellow vein virus (BNYVV) in the ER”
Claim · model-written summary
The TGB proteins of BNYVV are ubiquitinated by NbHRD1 in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Gene
- NbHRD1e1
- Outcome
- TGB proteins are ubiquitinatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The TGB proteins are ubiquitinated by NbHRD1 and then degraded by the UPS”
e2“overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana”
Claim · model-written summary
Overexpression of NbHRD1a significantly inhibited BNYVV infection in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- overexpression of NbHRD1ae1
- Interactor
- BNYVVe1pathogen
- Outcome
- BNYVV infectione1
- Direction
- inhibitse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana”
Claim · model-written summary
Silencing of NbHRD1 promoted BNYVV infection in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- silencing of NbHRD1e1
- Interactor
- BNYVVe1pathogen
- Outcome
- BNYVV infectione1
- Direction
- promotese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana”
Claim · model-written summary
NbHRD1a mainly impairs BNYVV cell-to-cell movement rather than virus replication in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Treatment
- NbHRD1ae1
- Interactor
- BNYVVe1pathogen
- Outcome
- BNYVV cell-to-cell movemente1
- Direction
- impairse1
- Comparator
- virus replicatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“NbHRD1a mainly impairs BNYVV cell-to-cell movement, rather than virus replication”
e2“overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana”
Claim · model-written summary
NbHRD1 targets the TGB proteins of potato virus X for ubiquitination and inhibits virus infection.
- Taxon
- N. benthamianaNicotiana benthamianae2
- Interactor
- potato virus XPotato virus Xe1pathogen
- Gene
- NbHRD1e1
- Outcome
- virus inhibitione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“NbHRD1 also targets the TGB proteins of potato virus X for ubiquitination and virus inhibition”
e2“overexpression of NbHRD1a significantly inhibits BNYVV infection, whereas silencing of NbHRD1 promotes BNYVV infection in N. benthamiana”
The same questions in other papers
loss of function · organism · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 7 in the opposite direction
- positive
- negative
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.